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博碩士論文 etd-0710102-132118 詳細資訊
Title page for etd-0710102-132118
論文名稱
Title
綠島產叢狀穗花珊瑚與多刺棘穗軟珊瑚化學成分及癌細胞毒殺活性之研究
Chemical Constituents and Cytotoxicity of Formosan Soft Corals Lemnalia Cervicorni and Dendronephthya spinifera
系所名稱
Department
畢業學年期
Year, semester
語文別
Language
學位類別
Degree
頁數
Number of pages
68
研究生
Author
指導教授
Advisor
召集委員
Convenor
口試委員
Advisory Committee
口試日期
Date of Exam
2002-05-29
繳交日期
Date of Submission
2002-07-10
關鍵字
Keywords
綠島產多刺棘穗軟珊瑚、化學成分及、綠島產叢狀穗花珊瑚
Chemical Constituents and Cytotoxicity, Formosan soft coral Dendronephthya spinifera, Formosan soft coral Lemnalia cervicorni
統計
Statistics
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The thesis/dissertation has been browsed 5704 times, has been downloaded 2419 times.
中文摘要
綠島產叢狀穗花珊瑚(Lemnalia cervicorni May)之二氯甲烷層粗萃物,經層析分離後得到4個sesquiterpenoids化合物,分別為GN40-29 (1)、GN40-79 (3)、GN40-169 (4)、GN40-173 (5)。而GN40-29a (2)是GN40-29 (1)乙醯化反應得到的產物。其中GN40-79 (3)、GN40-169 (4)、GN40-173 (5)為ylangene骨架的新化合物。
綠島產多刺棘穗軟珊瑚(Dendronephthya spinifera Holm) 之二氯甲烷層粗萃物,經層析分離後得到1個cadinene骨架的sesquiterpene化合物GN43-4 (6),為一新化合物。


Abstract
Chromatographic separation of methylene chloride extracts of Formosan soft coral Lemnalia cervicorni May (collected at Green Island off Taiwan) led to the isolation of four ylangene sesquiterpenoids, GN40-29 (1), GN40-79 (3), GN40-169 (4), and GN40-173 (5). Acetylation of GN40-29 (1) gave a derivative GN40-29a (2). Compounds 3-5 were new compounds. Compound 1 showed cytotoxicity against HT-29 cell, while compound 5 was cytotoxic to P-388 cells.
The methylene chloride extracts of Formosan soft coral Dendronephthya spinifera Holm (collected at Green Island off Taiwan) were found to contain a new cadinene sesquiterpene, GN43-4 (6).


目次 Table of Contents
目錄

壹、 緒論
一、 前言
二、 Lemnalia屬軟珊瑚之天然物化學研究回顧
三、Dendronephthya屬軟珊瑚之天然物化學研究回顧
貳、叢狀穗花珊瑚化學成分之研究
一、材料之採集與鑑定
二、Lemnalia屬軟珊瑚之分類地位
三、叢狀穗花珊瑚化學成分之萃取與分離流程
四、叢狀穗花珊瑚化學成分之分離純化
(1) GN40-29 (1)之分離純化
(2) GN40-29a (2)之乙醯化
(3) GN40-79 (3)之分離純化
(4) GN40-169 (4)之分離純化
(5) GN40-173 (5)之分離純化
五、叢狀穗花珊瑚化學成分之結構解析
(1) GN40-29 (1)之結構解析
(2) GN40-29a (2)之結構解析
(3) GN40-79 (3)之結構解析
(4) GN40-169 (4)之結構解析
(5) GN40-173 (5)之結構解析
參、多刺棘穗軟珊瑚化學成分之研究
一、材料之採集與鑑定
二、Dendronephthya屬軟珊瑚之分類地位
三、多刺棘穗軟珊瑚化學成分之萃取與分離流程
四、多刺棘穗軟珊瑚化學成分之分離純化
(1) GN43-4 (6)之分離純化
五、多刺棘穗軟珊瑚化學成分之結構解析
(1) GN43-4 (6)之結構解析
肆、叢狀穗花珊瑚之生物活性
伍、結論
陸、參考文獻
七、附錄
一、儀器及材料
二、衍生物之製備
三、癌細胞毒殺活性測試之分析方法
四、MTT分析原理
參考文獻 References
參考文獻
1. 戴昌鳳,1989,台灣的珊瑚。台灣省政府教育廳。
2. 方力行,1989,珊瑚學。教育部聯合出版委員會。
3. Paul J. Scheupr, “Bioorganic Marine Chemistry”, 1987, Springer-
Verlag Berlin.
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5. Burce F. Bowden, John C. Coll and Sarah Jane Mitchell, “Studies of Australia soft corals. XⅥ two new sesquiterpenes from Lemnalia humesi”, Aust. J. Chem., 1980, 33, 681-684.
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7. Richard R., Willian Fenical, Bruce Tagle, John Clardy, “Neolemnaneand eremophilane sesquiterpenoids from the pacific soft coral Lemnalia africana”, Tetrahedron, 1981, 37, 2569-2573.
8. Yasuji Yamada, Kazuo Lguchi, Hiroyuki Kikuchi, Yasumasa Tsukitani, Steven A. Drexler, Jon Clardy, “Lemnalol, a new sesquiterpenoid from the soft coral Lemnalia tenuis Verseveldt”, Tetra. Lett., 1982, 23, 1063-1066.
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12. Jaroslaw Jurek and Paul J. Scheuer, “Sesquiterpenoids and norsesquiterpenoids from the soft coral Lemnalia africana”, J. Nat. Prod., 1993, 56, 508-513.
13. Min Zhang, Kanghou Long, Houming Wu, Kan Ma, “A novel diterpene glycoside from the soft coral of Lemnalia bournei”, J. Nat. Prod., 1994, 57, 155-160.
14. Min Zhang and Zhishu Hung, “Two new diterpene glycosides fron the soft coral Lemnalia bournei”, J. Nat. Prod., 1998, 61, 1300-1301.
15. R. B. Bates and V. P. Thalacker, “Nuclear magnetic resonance spectral parameters in bicyclo[3, 1, 1,]heptanes. α-Pinene, myrtenal, and verbenone”, J. Org. Chem., 1968, 33, 1730-1732.
16. Yasuhiko Tomono, Hiroshi Hirota, Yukimitsu Imahara, and Nobuhiro Fusetani, “Isogosterones A-D, antifouling 13, 17-secosteroids from an octocoral Dendronephthya sp.”, J. Org. Chem., 1999, 64, 2272-2275.
17. Yasuhiko Tomono, Hiroshi Hirota, Yukimitsu Imahara, and Nobuhiro Fusetani, “Four new steroids from two octocorals”, J. Nat. Prod., 1999, 62, 1538-1541.
18. Kazuko Yoshikawa, Satoe Kanekuni, Mika Hanahusa, Shigenobu Arihara, Tomihisa Ohta, “Polyhydroxylated sterols from the octocoral Dendronephthya gigantean”, J. Nat. Prod., 2000, 63, 670-672.
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