污染整治一直是環保上重要的課題，先前所使用的整治方法固然效果卓越，但是有著整治成本高與對環境造成二次傷害的風險。因此近年來生態學家大力提倡生物復育法，利用環境中的微生物來分解污染物。本實驗基於此目的，擬建立一套16S rDNA-DGGE 分析系統，用來分析石油污染現地土壤微生物菌相，並評估此系統在監測污染整治上的可行性。本實驗評估了三種DNA 萃取方法：KIT、Bead-beatig、Freezw-thaw 的萃取效率及
其菌相分析。結果顯示以KIT 法萃取效果最佳，且菌相最完整。本研究亦發現兩次不同時間但地點相同採樣的土壤微生物菌相確實有改變，顯示此污染場址的土壤現地微生物能接受外來的stress並自我調節。此現象顯示以生物復育法降解污染物確有其可行性，但仍需進行更進一步的探討

In this study, we had established a 16S rDNA-DGGE analys is system to detect the microbial community in petroleum polluted soil and assess the feasibility of using this system to monitor the bioremediation process. Three genomic DNA extracted methods, the KIT, the Bead-beating system, and the Freeze-thaw method were used
to evaluate the DNA extraction efficiency and purity. These DNA samples were further tested by DGGE to analysis the microbial community in soil samples. The results showed that KIT method performed advantageous not only in the DNA extraction efficiency and purity, but also expressed the richest bacterial community in its
PCR products. From the DGGE analysis, our data indicated that composition of bacterial community were different in the soil samples
that were taken from the same site but at different time. This indicated that the species and number of microorganisms in a polluted soil were under a dynamic transition. The combination of DGGE and 16S rDNA gene sequence analysis system were also proven useful in identifying the predominant microbes in a soil sample and monitoring its bacterial community.

中文摘要……………………………………………………..……..Ⅲ
英文摘要……………………………………………………..……..Ⅳ
第一章前言……………………………………………………..…..1
第二章材料方法…………………………………….…………….11
第三章結果……………………………………………………..…25
第四章討論………………………………………………………..31
第五章結論與建議………………………………………………..41
參考文獻…………………………………………………………....43
圖表…. ……………………………………………………………..50

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