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博碩士論文 etd-0712105-160403 詳細資訊
Title page for etd-0712105-160403
論文名稱
Title
海水中添加溴或氯離子的臭氧水對海水魚類致病菌殺菌效果之研究
Bacteriocidal Effects of Ozonated Seawater Added with Bromide or Chloride on Marine Fish Pathogens
系所名稱
Department
畢業學年期
Year, semester
語文別
Language
學位類別
Degree
頁數
Number of pages
72
研究生
Author
指導教授
Advisor
召集委員
Convenor
口試委員
Advisory Committee
口試日期
Date of Exam
2005-06-30
繳交日期
Date of Submission
2005-07-12
關鍵字
Keywords
臭氧、海水、致病菌、氯離子、溴離子、殺菌
chloride, bacteriocidal, ozone, bromide, seawater, pathogens
統計
Statistics
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中文摘要
臭氧為一具有強大氧化力的物質,具有殺菌、除臭、脫色等數種功用,運用在水族繁養殖業上可以提昇幼體孵化率、穩定水質以提昇養殖效益。本研究主要在探討海水中的氯離子(Cl-)與溴離子(Br-)對臭氧溶入量之影響,以靛藍法(indigo colorimetric method)檢測臭氧溶入水體後產生之總殘餘氧化物濃度(total residual oxidants, TROs)之變化,並選用三種常見之海洋致病菌,探討可否藉由添加Cl-或Br-提昇臭氧殺菌之功效。結果顯示,臭氧在逆滲透(RO)處理水中溶入量低,約為2.3 mg/L,在停止通入臭氧後,TROs濃度即迅速降至0.5 mg/L。臭氧通入海水的過程中,TROs會迅速上升,最高可達8.5 mg/L,然後再下降達到穩定值,濃度約為6 mg/L。Cl-添加之RO水中後,TROs濃度變動範圍在2-3.5 mg /L之間,且停止通入臭氧後,TROs亦會迅速降低,此即顯示海水中之Cl-無法有效提昇臭氧在水中的溶入量。在RO水中添加Br-後不但可以有效提昇臭氧在水中之溶入量,並可在停止通入臭氧後,維持水中TROs之濃度,且其濃度會隨Br-添加量的增加而提昇,此即顯示海水中之Br-可有效地提昇臭氧在海水中的溶入量。在海水中添加Br-與純海水相較之下,添加Br-可以有效提昇海水中TROs濃度的生成,並可維持停止臭氧通入後的濃度,不會下降。在殺菌效果方面,海水中添加Br-與純海水相較,對各菌株達到完全抑菌所需之TROs濃度均能夠有效降低或相似。因此,使用臭氧作為殺菌工具時,在海水飼育水中致病菌量大量繁生時,即可藉由添加少量之Br-,迅速提昇TROs濃度,以達到快速抑菌的功效,避免養殖生物受到感染,以減少損失。
Abstract
Ozone is a powerful oxidant which can be use for bacterial inactivation, deodorizing, and bleaching. In aquatic farms, it can improve larvae hatching and keep water quality under control. The primary object of this research is to investigate the influence of ozone solubility by the concentrations chloride(Cl-)and bromide(Br-)in seawater. The indigo colorimetric method was used to detect the variation of total residual oxidants(TROs)after ozonation. The bacteriocidal effects of ozone with added Cl- or Br- were tested on three common marine fish pathogens. The results showed that the ozone solubility in reverse osmosis(RO)treated water was about 2.3 mg/L, and rapidly decreased to 0.5 mg/L when ozonation stopped. The process of ozonation in seawater from start to equilibrium, the TROs concentration could reach 8.5 mg/L and then decreased slowly to a stable concentration of about 6 mg/L. When Cl- was added in RO water, the TROs concentration varied from 2 to 3.5 mg/L, and rapidly disappeared after stop ozonation. It was concluded that Cl- could not effectively increase ozone solubility in seawater. In contrast, adding Br- in RO water not only efficiently increased the solubility of ozone but also maintained the TROs concentrations after stop ozonation, and the TROs concentrations were proportional to the Br- concentrations. The same trends were also observed in seawater added with Br-. In the bacteriocidal effect of ozone, compare with pure seawater, adding of Br- in seawater could effectively reduce the TROs concentrations needed to completely inhibit each pathogen. Such practice may be extended to aquatic farms to eliminate or reduce the bacterial pathogens in seawater.
目次 Table of Contents
中文摘要………………………………………………………………….I
英文摘要……………………………………………………………….III
目錄……………………………………………………………………….V
表目錄…………………………………………………………………VII
圖目錄……………………………………………………………………IX

前言………………………………………………………………………1
材料與方法………………………………………………………………8
1、 實驗設備……………………………………………………8
2、 水中總殘餘氧化物(TROs)濃度檢測方法………………11
3、 實驗方法……………………………………………………13
實驗一:RO水通入臭氧與停止後之TROs濃度的變化……13
實驗二:海水中通入臭氧與停止後之TROs濃度的變化…14
實驗三:RO水中添加Cl-再通入臭氧與停止後對TROs濃度
的影響……………………………………………14
實驗四:RO水中添加不同量Br-再通入臭氧與停止後對
TROs濃度的影響…………………………………15
實驗五:海水中添加Br-對提昇TROs濃度之效果………15
實驗六:臭氧對三種常見海洋致病菌的殺菌效果………16
實驗七:海水添加Br-對提昇臭氧殺菌效果之可行性…17
4、 實驗結果分析方法…………………………………………17
結果……………………………………………………………………18
實驗一:RO水通入臭氧與停止後之TROs濃度的變化…………18
實驗二:海水中通入臭氧與停止後之TROs濃度的變化…………18
實驗三:RO水中添加Cl-再通入臭氧與停止後對TROs濃度的影
響…………………………………………………………19
實驗四:RO水中添加不同量Br-再通入臭氧與停止後對TROs濃度
的影響……………………………………………………20
實驗五:海水中添加Br-對提昇TROs濃度之效果………………21
實驗六:臭氧對三種常見海洋致病菌的殺菌效果………………21
實驗七:海水添加Br-對提昇臭氧殺菌效果之可行性…………22
討論……………………………………………………………………24
1、 臭氧於RO水中的反應………………………………………24
2、 RO水中添加Cl-對臭氧溶入後,對所產生之TROs濃度的影
響……………………………………………………………25
3、 RO水中添加Br-對臭氧溶入後,對所產生之TROs濃度的影
響……………………………………………………………27
4、 臭氧於海水中的溶入情形…………………………………28
5、 臭氧對各種海洋致病菌的殺菌效果………………………29
6、 添加Br-對各種海洋致病菌殺菌效果之影響……………32
7、 海水中添加Br-對TROs生成量之影響……………………33
8、 以臭氧殺菌時添加Br-的應用方法………………………34
9、 各種消毒法使用效益之比較………………………………35
10、 TROs濃度對養殖生物的毒害與解決方法…………………36
結論……………………………………………………………………38
參考文獻………………………………………………………………39
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