本研究探討Sprague-Dawley (SD)大鼠體內各時期蟲體對albendazole感受性差異。在用藥時間點部分，四組大鼠分別於感染後零週、一週、二週、三週餵食10mg/kg的albendazole，結果顯示感染後零週及一週施藥能壓抑廣東住血線蟲感染，與未施藥對照組比較後發現這兩組除了在平均回收蟲數明顯減少外，在相對心肺濕重部分也有顯著差異。於感染後六週施以不同劑量albendazole實驗中，在100mg/kg組中，用藥後兩周糞便中第一期幼蟲回收數目明顯減少，並持續至十一週。至於10mg/kg組及50mg/kg組部份，用藥後四週，糞便中的第一期幼蟲數目開始回升。此外，也藉由石蠟切片觀察受感染大鼠肺葉中肉芽腫的形成，發現第一期幼蟲的數目多寡與肉芽腫纖維化出現有密切關係。
在以ELISA偵測大鼠血液中抗各期蟲體的特異性抗體力價實驗中發現血清中第一期幼蟲與雌蟲特異性IgE在感染後第一週及第五週呈現高峰，血液中嗜伊紅性白血球也同樣出現這種現象，這顯示IgE力價和嗜伊紅性白血球數目的升高可能與抗體依賴性之細胞毒殺反應(antibody-dependent cell mediated cytotoxicity,ADCC)有關。

This study evaluates the efficiency of albendazole against different stages of Angiostrongylus cantonensis in Sprague-Dawley rats. Using a single oral dose of albendazole of 10 mg/kg at 0 or 1 or 2 or 3 weeks post-infection (PI) showed that the drug in 0 or 1 weeks PI was more effective in the larval infection. Compared to those in non-treated control, the rats treated 0 or 1 weeks PI showed significant reductions not only in the relative wet weight of heart and lungs (g/100 g body weight), but also in the mean number of adults recovered. With regard to single oral doses of 10 or 50 or 100 mg/kg at weekly intervals from 6 weeks PI, the rats treated 100 mg/kg in 2 weeks post-treatment (PT) showed significant reduction in the L1 in its feces and the amounts were lastingly few. In the rats treated 10 or 50 mg/kg, larval output reappeared from 4 weeks PT. The histomorphology of granuloma formation was also investigated in the lungs of SD rats infected with A. cantonensis　by paraffin section. It was observed that granulomatous fibros was strongly associated with the number of L1 in the lungs.
IgG and IgE antibodies specific for antigens from L1, L5, male worms or female adults of A. cantonensis were respectively measured by an enzyme-linked immunosorbent assay (ELISA). The specific IgE response to antigens from L1 or female adults of A. cantonensis in the serum reached the maximum at 1 and 5 weeks PI. Similarly, the eosinophil response also revealed remarkable peaks at 1 and 5 weeks PI. These results might prove that the increases of IgE titer and eosinophil are related to antibody-dependent cell mediated cytotoxicity (ADCC).

摘要.....................................................Ⅰ
英文摘要.................................................Ⅱ
目錄.....................................................Ⅳ
圖表目錄.................................................Ⅴ
前言......................................................1
材料與方法................................................6
結果.....................................................13
討論.....................................................22
參考文獻.................................................27
附表.....................................................33
附圖.....................................................40

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