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博碩士論文 etd-0715105-172635 詳細資訊
Title page for etd-0715105-172635
論文名稱
Title
INF1 蛋白在引發菸草過敏反應時之陽性POD 表現的影響
Effect of INF1 Protein on Cationic Peroxidase Genes in Tobacco Leaves during Hypersensitive Response
系所名稱
Department
畢業學年期
Year, semester
語文別
Language
學位類別
Degree
頁數
Number of pages
41
研究生
Author
指導教授
Advisor
召集委員
Convenor
口試委員
Advisory Committee
口試日期
Date of Exam
2005-05-26
繳交日期
Date of Submission
2005-07-15
關鍵字
Keywords
INF1、過氧化氫、peroxidase、過敏反應、煙草、木質素、laccase
hypersensitive response, lignin, hydrogen, INF1, tobacco
統計
Statistics
本論文已被瀏覽 5681 次,被下載 2319
The thesis/dissertation has been browsed 5681 times, has been downloaded 2319 times.
中文摘要
以INF1蛋白處理完全展開的煙草葉片,12小時內即可看見HR損傷面積。利用IEF可看見陽性POD的增加,尤其是pI 8.7 POD。在木質素的測定上可看出POD活性增加和木質素的合成有關。而在陽性和陰性的POD transcripts增加和POD的活性增加相關。過氧化氫的減少應為被引發的POD所消耗。在我們的研究中,推測laccase作用在HR的早期,而當時間的延長,POD和laccase 則共同作用在木質素合成上。
Abstract
In our investigation, fully expanded tobacco leaves were used to study the hypersensitive response caused by the inoculation of INF1 purified from E. coli strains DH5α
目次 Table of Contents
Abstract in Chinese ----------------------------- I Abstract in English ------------------------------II
Table of contents --------------------------------III
List of figures -----------------------------------IV Introduction ------------------------------------- 1 Materials and Methods ---------------------------- 5
Results -------------------------------------------12 Discussion --------------------------------------- 17 References --------------------------------------- 20 Figures -------------------------------------------25
參考文獻 References
S. Baccouch, A. Chaoui, E.E. Ferjani, Nickel-induced oxidative damage and antioxidant responses in Zea mays shoots, Plant Physiol. Biochem. 36 (1998) 689-694. M.R, Bajar AM, Kolattukudy PE (1993) Induction of a tomato anionic peroxidase gene (tap1) by wounding in transgenic tobacco and activation of tap1/GUS and tap2/GUS chimeric gene fusions in transgenic tobacco by wounding and pathogen attack, Plant Mol Biol 21: 341–354 A.R. Barceló, G.J. Aznar-Asensio, Coniferyl alcohol oxidase activity of a cell-wall-located class III peroxidase, Aust. J. Plant Physiol. 26 (1999) 411-419. C.S. Bestwick, I.R. Brown, J.W. Mansfield, Localized changes in peroxidase activity accompany hydrogen peroxide generation during the development of a nonhost hypersensitive reaction in lettuce, Plant Physiol. 118 (1998) 1067-1078. K.A. Blee, J.W. Choi, A.P. O’Connell, W. Schuch, N.G. Lewis, G.P. Bolwell, A lignin-specific peroxidase in tobacco whose antisense suppression leads to vascular tissue modification, Phytochemistry 64 (2003) 163-176 W. Boerjan, J. Ralph, M. Baucher, Lignin biosynthesis, Annu. Rev. Plant Biol. 54 (2003) 519-546. D.J. Bradley, P. Kjellbom, C.J. Lamb, Elicitor- and wound-induced oxidative cross-linking of a proline-rich plant cell wall protein: A novel, rapid defense response, Cell 70 (1992) 21-30. R.J. Bruce, C.A. West, Elicitation of lignin biosynthesis and isoperoxidase activity by pectic fragments in suspension cultures of castor bean, Plant Physiol. 91 (1989) 889-897. A.C. Cazalé, M.A. Rouet-Mayer, H. Barbier-Brygoo, Y. Mathieu, L. Christiane, Oxidative burst and hypoosmotic stress in tobacco cell suspensions, Plant Physiol. 116 (1998) 659-669. C.H. Chen, H.J. Lin, M.J. Ger, D. Chow, T.Y. Feng, cDNA cloning and characterization of a plant protein that may be associated with the harpinpss-mediated hypersensitive response, Plant Mol. Biol. 43 (2000) 429-438. J.H. Christensen, G. Bauw, K.G. Welinder, M. van Montagu,W. Boerjan, Purification and characterization of peroxidases correlated with lignification in Poplar xylem, Plant Physiol.118 (1998) 125-135. M.D. Curtis, J.P. Nourse, J.M. Manners, Nucleotide sequence of a cationic peroxidase gene form the tropical forage legume Stylosanthes humilis, Plant Physiol. 108
20
(1995) 1303-1304. Dawson JH (1988) Probing structure-function relations in hemecontaining oxygenases and peroxidases, Science 240: 433–439 S. Dorey, M. Kopp, P. Geoffroy, B. Fritig, S. Kauffmann, Hydrogen peroxide from the oxidative burst is neither necessary nor sufficient for hypersensitive cell death induction, phenylalanine ammonia lyase stimulation, salicylic acid accumulation, or scopoletin consumption in cultured tobacco cells treated with elicitin, Plant Physiol. 121 (1999) 163-171. C.H. Foyer, H. Lopez-Delgado, J.F. Dat, I.M. Scott, Hydrogen peroxide- and glutathione-associated mechanisms of acclimatory stress tolerance and signaling, Physiologia Plantarum 100 (1997) 241–254. S.M. Gallego, M.P. Benavides, M.I. Tomaro, Effect of heavy metal ion excess on sunflower leaces: evidence for involvement of oxidative stress, Plant Sci. 121 (1996) 151-159. B. Gavnholta, K. Larsenb, Molecular biology of plant laccases in relation to lignin formation, Physiologia Plantarum 116 (2002) 273–280. M.J. Ger, C.H. Chen, S.Y. Hwang, H.E. Huang, A.R. Podile, B.V. Dayakar, T.Y. Feng, Constitutive expression of hrap gene in transgenic tobacco plant enhances resistance against virulent bacterial pathogens by induction of a hypersensitive response, Mol. Plant-Miocrobe Inter. 15 (2002) 764-773. Y. Ichinose, S. Andi, R. Doi, R. Tanaka, F. Taguchi, M. Sasabe,K. Toyoda, T. Shiraishi, T.Yamada, Generation of hydrogen peroxide is not required for harpin-induced apoptotic cell death in tobacco BY-2 cell suspension culture, Plant Physiol. Biochem. 39 (2001) 771–776. S. Kamon, P. van West, V.G.A.A. Vleeshouwers, K.E. de Groot, F. Govers, A gene encoding a protein elicitor of Phytophthora infestans is down-regulated during infection of potato, Mol. Plant-Microb. Inter. 10 (1997) 13-20. S. Kamon, H. Lindqvist, F. Govers, A novel class of elicitin-like genes from Phytophthora infestans, Mol. Plant-Microb. Inter. 10 (1997) 1028-1030. S. Kamon, P. van West, V.G.A.A. Vleeshouwers, K.E.de Groot, F. Govers, Resistance of Nicotiana benthamiana to Phytophthora infestans is mediated by the recognition of the elicitor protein INF1, Plant Cell 10 (1998) 1413-1425. A. Kärkönen, S. Koutaniemi, M. Mustonen, K. Syrjänen, G. Brunow, I. Kilpeläinen, T.H. Teeri, L.K. Simola, Lignification related enzymes in Picea abies suspension cultures, Physiol. Plant. 114 (20002) 343-353.
21
A. Kawaoka, E. Matsunaga, S. Endo, S. Kondo, K. Yoshida, A. Shinmyo, H. Ebinuma, Ectopic expression of a horseradish peroxidase enhances growth rate and increase oxidative stress resistance in Hybrid Aspen, Plant Physiol. 132 (2003) 1177-1185. A. Levine, R. Tenhaken, R. Dixon, C. Lamb, H2O2 from oxidative burst orchestrates the plant hypersensitive disease resistance response, Cell 79 (1994) 583-593. N.G. Lewis, L.B. Davin, S. Sarkanen, The nature and function of lignins, in: Barton DHR, Nakasaki K, Methcohn O (Eds.), Comprehensive Natural Products Chemistry, Vol. 3, Elsevier Science, New York, 1999, pp. 617–745. L.M. Lagrimini, S. Rothstein, Tissue specificity of tobacco peroxidase isozymes and their induction by wounding and tobacco mosaic virus infection, Plant Physiol. 84 (1987) 438-442. L.M. Lagrimini, W. Burkhart, M. Moyer, S. Rothstein, Molecular cloning of complementary DNA encoding the lignin-forming peroxidase from tobacco: Molecular analysis and tissue-specific expression, Proc. Natl. Acad. Sci. USA 84 (1987) 7542-7546. L.M. Lagrimini, Wound-induced deposition of polyphenols in transgenic plants overepressing peroxidase, Plant Physiol. 96 (1991) 577-583. L.M. Lagrimini, V. Gingas, F. Finger, S. Rothstein, T.T.Y. Liu, Characteriztion of antisense transformed plants deficient in the tobacco anionic peroxidase, Plant Physiol. 114 (1997) 1187-1196. Z.H Liu, Ger MJ (1997) Changes of enzyme activity during pollen germination in maize, and possible evidence of lignin synthesis, Aust J Plant Physiol 24: 329–335 M. Mäder, Compartmentation of peroxidase isoenzymes in plant cells, in: C. Penel, T. Gaspar, H. Greppin (Eds), Plant Peroxidases 1980-1990, University of Geneva, Switzerland, 1992, pp. 37-46. A.M. Mayer, R. C. Staples, Laccase: new functions for an old enzyme, Phytochemistry 60 (2002) 551-565. P.D. Olson, J.E. Varner, Hydrogen peroxide, Plant J. 4 (1993) 887-892. F. Pomar, N. Caballero, M.A. Pedreño, A.R. Barceló, H2O2 generation during the auto-oxidation of coniferyl alcohol drives the oxidase activity of a highly conserved class III peroxidase involved in lignin biosynthesis, FEBS Lett. 519 (2002) 198-202. M. Quiroga, C. Guerrero, M.A. Botella, A. Barceló, I. Amaya, M.I. Medina, F.J. Alonso, S.M. Forchetti, H. Tigier, V. Valpuesta, A tomato peroxidase involved in
22
the synthesis of lignin and suberin, Plant Physiol. 122 (2000) 1119-1127. P. Ranocha, G. McDougall, S. Hawkins, R. Sterjiades, G. Borderies, D. Stewart, M. Cabanes-Macheteau, A.M. Boudet, D. Goffner, Biochemical characterization, molecular cloning and expression of laccases—a divergent gene family—in poplar, Eur. J. Biochem. 259 (1999) 485–495. P. Ranocha, M. Chabannes, S. Chamayou, S. Danoun, A. Jauneau, A-M. Boudet, D. Goffner, Laccase down-regulation causes alterations in phenolic metabolism and cell wall structure in poplar, Plant Physiol. 129 (2002) 145-155. K.D. Richards, E.J. Schott, Y.K. Sharma, K.R. Davis, R.C. Gardner, Aluminum induces oxidatives stress genes in Arabidopsis thaliana, Plant Physiol. 116 (1998) 409-418. A. Richardson, G.J. McDougall, A laccase-type polyphenol oxidase from lignifying xylem of tobacco, Phytochemistry 44 (1997) 229-235. M. Sasabe, K. Takeuchi, S. Kamoun, Y. Ichinose, F. Govers, K. Toyoda, T. Shiraishi, T.Yamada, Independent pathways leading to apoptotic cell death, oxidative burst and defense gene expression in response to elicitin in tobacco cell suspensions culture, Eur. J. Biochem. 267 (2000) 5005–5013. R. Sterjiades, J.F.D. Dean, K.E.L. Eriksson, Laccase from sycamore maple (Acer pseudoplatanus) polymerizes monolignols, Plant Physiol. 99 (1992) 1162-1168. R. Stejiades, J.F.D. Dean, G. Gamble, D.S. Himmelsbach, K.E.L. Eriksson, Extracellular laccase and peroxidase from sycamore maple (Acer pseudoplatanus) cell suspension cultures, Planta 190 (1993) 75-87. F. Taguchi, R. Shimizu, R. Nakajima, K. Toyoda,T. Shiraishi,Y. Ichinose, Differential effects of flagellins from Pseudomonas syringae pv. tabaci, tomato and glycinea on plant defense response, Plant Physiol. Biochem. 41 (2003) 165–174. R. Tenhaken, A. Levine, L.F. Brisson, R.A. Dixon, C. Lamb, Function of the oxidative burst in hypersensitive disease resistance, Proc. Natl. Acad. Sci. USA 92 (1995) 4158-4163. W.C. Wang, Z.H. Liu, Harpinpss-induced peroxidase and lignin accumulation in tobacco during the hypersensitive response, Aust. J. Plant Physiol. 26 (1999) 265-272. R. Whetten, R. Sederoff, Lignin biosynthesis, Plant cell 7 (1995) 1001-1013. T. Yahraus, S. Chandra, L. Legendre, P.S. Low, Evidence for a mechanically induced oxidative burst, Plant Physiol. 109 (1995) 1259-1266. X.S. Ye, S.Q. Pan, J. Kuc, Activity, isozyme pattern, and cellular localization of 23
peroxidase as related to systemic resistance of tobacco to blue mold (Peronospora tabacina) and to tobacco mosaic virus, Phytopathology 80 (1990) 1295-1299.
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