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博碩士論文 etd-0716104-150251 詳細資訊
Title page for etd-0716104-150251
論文名稱
Title
中華黏盲鰻Eptatretus chinensis (Myxinidae, Myxiniformes)分類與生殖生物特徵之研究
Taxonomiy and Reproduction Biology of Eptatretus chinensis (Myxinidae, Myxiniformes)
系所名稱
Department
畢業學年期
Year, semester
語文別
Language
學位類別
Degree
頁數
Number of pages
110
研究生
Author
指導教授
Advisor
召集委員
Convenor
口試委員
Advisory Committee
口試日期
Date of Exam
2004-06-17
繳交日期
Date of Submission
2004-07-16
關鍵字
Keywords
中華黏盲鰻
Myxiniformes, Eptatretus chinensis, Myxinidae
統計
Statistics
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中文摘要
中華黏盲鰻 (Eptatretus chinensis )之形態變異與系統分類在材料缺乏的情況下,而了解甚少,本研究是利用最近獲得該種之額外新鮮標本,對其(1)形態變異加以調查,(2)利用mtDNA 16S rRNA序列資料探究牠與其他3屬15種種盲鰻間之親緣關係,(3)對其漁業生物相關做基礎的研究,其中包括 (雌雄比、成熟體長、抱卵情況,以解月間之變化及產卵期之估測。這些資料可做E. chinensis漁業情況監測之用。根據形態上之黏液孔的數量與分佈、腹大動脈分支的位置之特徵,本種與沈氏副盲鰻 (Paramyxine sheni) 最為相似。mtDNA 16S rRNA 片段之比較結果支持中華黏盲鰻之親緣關係與西太平洋台灣海域之種類沈氏副盲鰻, Quadratus yangi, Q. taiwanae, Q. nelsoni較近,而與西太平洋台灣海域布氏黏盲鰻 (E. burgeri)、大洋洲之E. cirrhatus及東太平洋之E. stouti關係較遠。該種之漁業生物參數,在不同樣品體長-體重迴歸關係分析結果顯示:雌、雄之體長-體重的相對成長速率關係顯著 (P<0.001) ,但雌性盲鰻斜率稍較雄性盲鰻大一些 (雄性於體長與體重的關係並非一定為正相關)。性比方面測驗顯示雌魚數量顯著多於雄魚。在各卵徑區間內卵數百分比分佈方面,根據大型卵(最大直徑大於30.0 mm)卵數的月別百分率分佈及卵袋出現的情況來看,中華黏盲鰻可能一年中有多次產卵活動, 5-6月可能為產卵之高峰期。測量329尾盲鰻中,體長232mm之雌魚開始出現2mm之魚卵,由數據顯示同一月份中卵徑大小有相當大的變異。雌魚平均卵數目可達25個,卵數愈多平均卵徑愈低。排卵後有卵袋 (白色透明卵袋)的情況,且觀察褐色疤痕及黃色圓點,307-461 mm體長之個體有卵袋 (5-20 mm) 的出現。此外,腸繫膜還有1-4 mm之褐色疤痕在腸繫膜上。若由切片之觀察所得之結果,褐色疤痕外膜內層有皺摺的現象,皺摺構造間有顆粒物質,推測褐色疤痕為萎縮之卵袋。黃色圓點在組織形態上與褐色疤痕不同。
Abstract
The purposes of this study are to elucidate the taxonomy and reproductive biology of Eptatretus chinensis. It includes three parts: (1) Description of external and internal taxonomic morphological characters. (2) Erection of hypothesis for the phylogenetic position of E. cheninsis using mtDNA 16S rRNA gene sequences. (3) Data on reproductive biology (male/female ratio, body length, monthly changes of eggs and spawn stage). Morphologically characters E. cheninsis and Paramyxine sheni are must similar to each other (number and distribution of slime pore in the brachial region and site of bifurcation of the ventral aorta). Sequences of the 16S rRNA indicate that
E. cheninsis is more closely related to P. sheni, Quadratus nelsoni, Q. taiwanae, and Q. yangi than to its Eptatretus congeners including the sympatric E. burgeri, the eastern Pacific Ocean E. stoutii and the Oceania E. cirrhatus. Additionally, the sequence is most similar to that of P. sheni. 329 specimens were sampled during January to December, 2003. Relationship between body length and body weight is significant (P<0.001). There were more females than males in the samples. According to the occurrences of large eggs (i.e., diameter > 30mm) and egg sacs in the sampling period, it is suggested that E. chinensis spawned through out the year with a possible spawning peak in May to June. Small eggs (< 2 mm) were found in specimen as small as 232 mm. Diameters of eggs carried by a female was variable. The average numbers of large eggs was 28.5, and there was a negative relationship between egg number and egg diameter. Egg sacs (5-20mm), brown scars (1-4mm) and yellow spots (<1mm) were found on the mesenteric. Egg sacs were found in 307-461 mm females. The histological sections of the brown scar (20mm) and large egg sac (10mm), exhibited folding of membranous structures and the particles among the sac structures were similar. Therefore, it is suggested that the brown scar is a sympatric egg sac. The yellow spots and brown scars differed in their histological characters.
目次 Table of Contents
目 錄
章次 頁碼
中文摘要……………………………………………………………… i
英文摘要……………………………………………………………… iii
目錄…………………………………………………………………… v
表目錄………………………………………………………………… vi
圖目錄………………………………………………………………… vii
表附錄………………………………………………………………… ix
圖附錄………………………………………………………………… x
壹、前言……………………………………………………………… 1
貳、研究材料與方法………………………………………………… 6
(一)標本採樣…………………………………………………..… 6
(二)粒線體去氧核糖核酸序列分析…………………………..… 6
(三)序列數據之分析…………………………………………….. 9
(四)形質測量及分析…………………………………………..…10
參、結果………………………………………………………………11
(一)形態學方面之研究………………………………………….11
(二)分子生物學方面之研究…………………………………….11
(三)漁業生物相關之生殖特徵………………………………….13
肆、討論………………………………………………………………15
(一)形態學方面之研究………………………………………….15
(二)分子生物學方面之研究…………………………………….16
(三)漁業生物相關之生殖特徵………………………………….16
參考文獻………………………………………………………………19
個人履歷………………………………………………………………98
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