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博碩士論文 etd-0717103-192917 詳細資訊
Title page for etd-0717103-192917
論文名稱
Title
靜脈注射脂多醣引發大鼠胃發炎之組織病理研究
Histopathological Study on Inflammatory Stomach after Intravenous Injection of Lipopolysaccharide in Rats
系所名稱
Department
畢業學年期
Year, semester
語文別
Language
學位類別
Degree
頁數
Number of pages
62
研究生
Author
指導教授
Advisor
召集委員
Convenor
口試委員
Advisory Committee
口試日期
Date of Exam
2003-06-26
繳交日期
Date of Submission
2003-07-17
關鍵字
Keywords
發炎反應、大鼠、脂多醣、胃
Lipopo, Stomach, Inflammatory, ysaccharide, LPS
統計
Statistics
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中文摘要
引起炎症反應的因子有很多,例如:物質P、組織胺、辣椒素和內毒素(LPS)等。LPS 是來自於格蘭氏陰性細菌的成份,它可以刺激免疫細胞釋出發炎前驅物質細胞介素而引起組織的急性炎症反應或者全身性敗血症。現在的研究是去探討和研究在大鼠予以靜脈注射高劑量的內毒素後,其胃的分泌部和非分泌部的廣大的血漿外洩及微小血管滲漏的位置。使用印地安墨汁和Evans blue 二種追蹤染料,作為給予內毒素後計量廣大的血漿外洩血管的追蹤染劑。在銀染後的整裝標本胃的分泌部和非分泌中,可觀察到血管內的內皮細胞的內皮隙,Alcian blue和 PAS染色的組織切片則用來展現胃分泌部組織其黏膜細胞表面黏液的出現。
研究結果證明,大鼠注射LPS引起胃的分泌部和非分泌部血漿外洩比控制組範圍較廣大。在注射LPS 過了30分鐘內有血漿外漏,尤其是在開始的前5分鐘。在注射LPS 5、30分鐘胃的分泌部中可以發現其微血管後小靜脈和收集小靜脈有多量的內皮隙形成。胃的分泌部組織切片顯示滲漏血管出現在漿膜層和肌層的外面。注射LPS亦能造成胃腺細胞釋放黏液。結論是內毒素所引起的血漿外洩與內皮隙的形成是相關的,並且與表層黏液從黏液細胞釋放有關。
Abstract
There are a lot of inflammatory agents, such as substance P, histamine, capsaicin, and lipopolysaccharide(LPS). LPS is the component of all gram-negative bacterial cell wall that can stimulate the immune cells to release pro-inflammatory cytokines that can induce systemic acute inflammation and sepsis. The present study sought to investigate the location of leaky microvessels and magnitude of plasma leakage in the rat stomach secretory and non-secretory portions after an intravenous injection of high dose of LPS(15 mg/kg). India ink(1 ml/kg) and Evans blue(30 mg/ml)were used as tracer dyes to measure the magnitude of plasma leakage after LPS application. In the whole mounts of the stomach secretory and non-secretory portions with silver staining, the boundaries of endothelial cells and gaps between endothelial cells for plasma leakage in the blood vessels of microcirculation were made visible. Tissue sections were stained with Alcian blue and periodic acid-Schiff reagent to reveal the mucosubstance present in the mucous cells of the stomach secretory tissue.
The result of study demonstrated that the magnitude of LPS-induced plasma leakage in the rat stomach secretory and non-secretory portions was larger than that of the vehicle control. Extensive plasma extravasation was found from 5 min to 30 min after LPS injection. Numerous endothelial gaps formed in both postcapillary venules and collecting venules in the rat stomach secretory portion at 5 min and 30 min after LPS, but the number of gaps declined strikingly 60 min after LPS. Endothelial gaps were rare in the stomach of vehicle control. Histological sections of the stomach secretory portion showed that the leaky vessels were present in the serosa and muscularis externa. Administration of LPS also resulted in release of mucus in gastric gland cells. It is concluded that endotoxin-induced increase in plasma leakage correlated with the formation of endothelial gaps, and associated with depletion of mucosubstance from the mucous cells.
目次 Table of Contents
目錄(Index)
【Abstract】 1
【中文摘要】 3
【緒言】 4
一、炎症反應(Inflammation) 4
二、內毒素 (Endotoxin) 6
三、胃的解剖學及組織病理學研究 7
【研究目的】 10
【材料與方法】 11
一、實驗材料 11
實驗動物 11
藥品及溶液配製 11
二、動物實驗與標本處理過程 12
1. 股靜脈注射內毒素誘發腸胃道炎症反應 12
2. 動物組織灌流處理 14
3. 銀染色法 15
4. 胃整體封埋(whole mount)標本製作 16
5. 胃組織切片製作 16
6. Alcian blue-PAS染色 17
三、血漿滲漏程度之研究 18
1. 印地安染劑標識之滲漏血管形態計量分析 18
2. Evans blue之化學定量分析 18
3. 銀染標示發炎血管的內皮隙數目之分析 20
四、統計分析 20
【結果】 21
一、內毒素誘發胃血漿外洩作用:以印地安墨汁為染劑 21
1. 胃整裝標本的微循環的顯微觀察 21
2. 標示印地安墨汁的血管形態計量分析 22
二、內毒素誘發胃血漿外洩作用:以萃取Evans blue做化學定量分析 25
三、以銀染色標示發炎小靜脈的內皮隙 26
1. 銀染色整裝標本微循環之顯微觀察 26
2. 銀染標示血管內皮隙數目的統計分析 27
四、胃分泌部組織切片之顯微觀察 28
1. 以Alcain blue-PAS染色之切片觀察 28
2. Toluidine blue 染色 29
【討論】 30
一、高劑量靜脈注射LPS引起胃分泌部及非分泌部之血漿滲漏時程變化 30
二、高劑量靜脈注射LPS引起小靜脈內皮組織的內皮隙數目之變化與血漿外洩之關係 32
三、病理組織變化 33
【結論】 35
【參考文獻】 36
【圖】 41
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