DC2蛋白是先前本實驗室誘導老鼠腦幹死亡過程中發現的新未知基因之表現蛋白質。
經由生物資訊軟體分析發現，人及老鼠DC2蛋白均149個胺基酸，分子量約16.8 kDa。人及老鼠DC2蛋白的胺基酸序列只相差一個胺基酸，均為穿膜蛋白，穿膜三次。顯示二者高度相似。在各正常組織，DC2蛋白均有不同程度的蛋白質表現，其中人的肝臟DC2蛋白表現相對於其他器官較多，排名第四名，而老鼠肝臟排名較後面，為第23名。Shibatani et al (Shibatani et al，2005) 經質譜分析(mass spectrometry)，認為DC2蛋白為寡醣轉移複合體(oligosaccharyltransferase complex; OST complex) 的次單位，參與醣化反應 (Glycosylation)。
將會誘導肝臟纖維化的硫代乙醯胺(Thioacetamide；TAA)加入老鼠飲用水，使老鼠肝臟纖維化。分析TAA誘導老鼠肝臟纖維化過程中，老鼠肝臟DC2蛋白之mRNA及protein表現情況，結果發現老鼠肝臟DC2 蛋白之mRNA expression ：（1）在無TAA作用下是有表現；（2）在TAA作用下老鼠肝臟DC2 protein之mRNA expression有逐漸增加的趨勢 ；而蛋白質的表現則（1）在無TAA作用下是有DC2蛋白表現；（2）在TAA作用下老鼠肝臟DC2 protein表現有增加的現象。

Gene of DC2 protein, a novel unknown gene, was identified previously in our laboratory while studying the death progression in the rat brain stem.
According to the search results of bioinformatics database, both human DC2 and house mouse DC2 are 149 amino acids long and 16.8 kDa. The entire sequence of human DC2 differs from house mouse DC2 by only a single amino acid substitution. The bioinformatics revealed that human DC2 and house mouse DC2 had three predicted transmembrane regions. These results suggest human DC2 and house mouse are highly homologous.
DC2 protein expresses differentially between organs. Human liver is the top fourth DC2-expressed organ, while house mouse liver is ranked 23rd DC2-expressed organ. Shibatani et al (Shibatani et al，2005) proposed DC2 protein as a potential subunit of mammalian Oligosaccharyltransferase (OST) after mass spectrometry analysis and suggested DC2 might involve in glycosylation.
House mouse liver fibrosis was induced by giving 300mg/L thioacetamide (TAA) in the drinking water for different periods of time, and then gene expression of house mouse DC2 of liver was analyzed. mRNA expression was found in normal house mouse liver and mRNA expression increased gradually after TAA administration. DC2 protein also found in normal house mouse liver and DC2 protein of house mouse liver increased after TAA administration.

中文摘要------------------------------------------------------------------------------------ 1
英文摘要------------------------------------------------------------------------------------ 3
壹、 緒論
1. 肝纖維化簡介--------------------------------------------------------------- 4
2. 硫代乙醯胺TAA (thioacetamide)使肝纖維化之機制-------------- 7
3. 什麼是DC2 蛋白--------------------------------------------------------- 9
4. 動物模式與醫學研究----------------------------------------------------- 10
5. 差異表現基因(Differentially expressed gene)--------------------- 12
6. 生物資訊 (Bioinformatics)---------------------------------------------- 13
貳、 實驗目的----------------------------------------------------------------------------- 15
參、 實驗材料與方法
1. 生物資訊分析-------------------------------------------------------------- 16
2. 檢體：肝纖維化之誘發-------------------------------------------------- 17
3. RT-PCR--------------------------------------------------------------------- 17
4. Western blotting----------------------------------------------------------- 20
肆、 結果與討論
1. 生物資訊分析結果-------------------------------------------------------- 24
2. DC2在TAA 作用下之mRNA的表現-------------------------------- 29
3. DC2在TAA 作用下之Western blotting的結果------------------- 34
伍、 結論------------------------------------------------------------------------------ 37
陸、 附圖------------------------------------------------------------------------------ 39
柒、 參考資料----------------------------------------------------------------------- 64

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