本研究探討以不同比例亞麻仁油和玉米油取代魚油對海鱺成長、肝臟中脂肪酸去飽和酵素及延長酵素基因表現量、組織中脂肪酸組成及免疫反應的影響。實驗飼料為等氮及等能量，酪蛋白和去油脂魚粉為蛋白源，油脂含量為15%，餵飼初重25g的海鱺八週後，全魚油飼料組的魚體增重率顯著高於其他取代飼料組〈取代量33-100%〉，餵食全植物油飼料者增重率最低，且肝體比和肥滿度皆最小，魚體脂肪含量也明顯低於其他各組。肝臟中脂肪酸去飽和酵素及延長酵素mRNA表現量並沒有隨著植物油取代量增加而有顯著改變。隨著植物油取代量增加，魚體組織中HUFA量有減少的趨勢，而PUFA量則隨著明顯增加。海鱺頭腎白血球呼吸猝發活性以餵食全魚油組最高，血清中溶菌酶酵素活性以餵食全植物油組較低；超氧歧化酵素活性以餵食全亞麻仁油組最高，而血清中天門冬胺酸轉胺酵素、丙胺酸轉胺酵素和替代路徑補體活性在各組間並沒有顯著差異。綜合以上結果，海鱺對飼料中魚油的需求相當高，且由PUFA合成HUFA的能力並不強，飼料中以亞麻仁油或玉米油或兩者取代魚油且取代率介於33-100%均造成海鱺成長低下，免疫反應也降低。

The effects of partial or total replacement of fish oil with linseed oil or corn oil or both in diets of cobia were valuated. Basal diet was isonitrogenous and isoenergetic and contained 15% crude lipid. Results of the 8-wk feeding trial show that fish fed diet containing only fish oil grew significant better than fish fed other replacement diets (replacement level 33-100%). Fish fed diet containing only plant oil grew the least and had the lowest liver weight, condition factor and body lipid concentration. Oil replacement did not significantly change liver mRNA gene expression of fatty acid desaturase and elongase. As levels of replacement increased, tissue PUFA increased while HUFA decreased. Fish fed all fish oil diet had the highest respiratory burst activities of head kidney phagocytes. Serum of the fish fed the all vegetable oil diets had the lowest lysozyme activities. Fish fed all linseed oil diet had the highest SOD activities. Serum alternative complement pathway activity, aspartate transaminase and alanine transaminase activity did not vary among treatments. The results show that cobia juveniles had relatively high need for fish oil in their diets, and the ability to synthesized HUFA from PUFA was limited. Partial or total replacement (33-100%) of fish oil with linseed oil or corn oil or both were detrimental to fish growth and immune responses.

中文摘要……………………………………………………………..………………Ⅰ
英文摘要…………………………………………………………………………..…Ⅱ
目錄………………………………………………………………………..…………Ⅲ
表目錄…………………………………………………………………………..……Ⅳ
圖目錄…………………………………………………………………………..……Ⅵ
文獻回顧………………………………………………………………………..……..1
前言…………………………………………………………………………………..12
材料與方法…………………………………………………………………………..14
結果…………………………………………………………………………………..36
討論…………………………………………………………………………………..51
參考文獻……………………………………………………………………………..56

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