Ketamine是一個常用於小兒麻醉的麻醉劑，更是時下青少年常見的濫用藥物，在動物模式ketamine證實會造成發育中幼鼠的腦神經細胞產生凋亡及日後行為異常。DHA屬於多元不飽和脂肪酸的一種，之前的文獻顯示DHA可以促進神經細胞的發育及抗凋亡的作用。本篇研究欲探討DHA是否具有抑制ketamine引起的神經膠瘤U87細胞死亡的作用。細胞存活分析發現ketamine具有劑量效應，濃度越高細胞存活率越低；DHA在濃度50μM以下（含）可以增加細胞存活率，50μM以上則會造成細胞死亡；DHA對中低劑量的ketamine具有保護效果，高劑量則沒有保護效果。而DHA產生保護效果時，可以觀察到p-AKT和p-ERK1/2表現量增加。Ketamine會造成p53、NFκB和BAX表現量增加，而經DHA治療後bcl-2呈現上升的情況。

Ketamine is an anesthetic commonly used in pediatric anesthetic, but it is also a drug that young people today commonly abuse. In the animal model ketamine is shown to cause apoptosis and abnormal behavior in the brain cells of developing rats in the future. DHA belongs to one of the polyunsaturated fatty acids. Previous literature shows that DHA may promote the development of and resistance to apoptosis of nerve cells. This research essay investigates the effects of DHA has a role in U87 glial tumor cell death induced inhibition of ketamine. Cell survival analysis showed that ketamine's effect is dose-dependent. The higher the concentration, the lower the cell viability. DHA in concentration 50μM (inclusive) can increase cell survival, 50μM above will cause cell death; DHA has a protective effect to low doses of ketamine, no protective effect to high doses. And when the DHA produces a protective effect, it can be observed that p-AKT and p-ERK1/2 exhibit increased performance capacity. Ketamine can cause an increase in the amount of p53 , NFκB and BAX performance, and after DHA treatment bcl-2 are showing a upward trend.

目錄
論文審定書-----------------------------------------i

誌謝--------------------------------------------------ii

中文摘要 -------------------------------------------iii

英文摘要--------------------------------------------iv

第一章、前言----------------------------------------1-7

第二章、材料與方法-------------------------------8-16

第三章、結果----------------------------------------17-20

第四章、討論----------------------------------------21-23

參考文獻----------------------------------------------24-30

圖表----------------------------------------------------31-40

附錄----------------------------------------------------41-48

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