摘要
本論文研究分佈在台灣西南海域之台灣特有種且為黏盲鰻亞科原始種之陳氏副盲鰻(Paramyxine cheni)之生殖生物學。從2002年12月到2003年11月期間，在台灣西南海域以蝦籠進行採樣，共捕獲337尾。所獲標本皆測量體長、體重，並以目測生殖腺方式進行性別鑑定，再計數卵數、卵徑大小及卵在腸繫膜上分佈之情形。本研究由生殖腺指數(GSI)發現陳氏副盲鰻全年都會產卵，但其生殖高峰是在四月至五月，六月開始有體長僅10到13公分之補充群的加入。陳氏副盲鰻雌、雄及未分化的個體比例為0.51：0.20:0.29。盲鰻的肝指數(HSI)為其生殖能量蓄積的指標，結果顯示其肝指數與生殖腺指數有正相關性。而肝臟粗脂肪含量在雌雄個體上有顯著的差異，雌個體比雄個體高。在月別上則是三月、六月與十一月有顯著的差異，十一月明顯的比其他兩個月份高。以冷凍切片觀察其肝臟中脂肪細胞分佈的情形，亦發現雌的P.cheni的肝脂肪細胞在生產前時會較緊密，而生殖季後則較為疏散。雌雄個體的肝臟外部形態，其色澤亦有明顯的差異，雌個體的色澤較為深色，雄個體較偏向於粉紅色。研究中測得P.cheni的最大體長為55.9公分，最小體長則有9.5公分。雌雌性P.cheni在18.5公分時，其生殖腺開始發育。以卵兩端出現鉤狀物做為最成熟度的判斷發現，最小的最成熟個體出現在40公分。透過卵細胞的透明化觀察其最成熟度，發現卵細胞的最成熟時期分佈在三、四月。卵徑的大小與其產卵後所遺留下來的痕跡(卵袋)大小亦有相似的趨勢。本研究中發現盲鰻幼期性別未分化時，由於生殖腺尚未發育，可由肝臟的顏色來判別雌雄個體。

Abstract
The Taiwanese hagfish Paramyxine cheni (Myxidae) is believed as a primitive species in the Eptatretinae and it distributes on the west south coast of Taiwan. Hagfishes are noteworthy to study from an evolutionary standpoint because they represent the oldest extant clade among the craniates. A better understanding of their reproductive states may thus reveal information for fisheries management. This research presents data on size, weight, GSI (Gonadosomatic Index), HSI ( Hepatosomatic index) and reproductive states for specimens collected at a study site (22°22’43”N, 120°14’92”E) near Tong-kung, Southern Taiwan. Specimens were collected once a months from December 2002 through November 2003 for body weight, body length, gonad weight, liver weight and liver lipid content. GSI, show that the P.cheni had a seasonal reproduction period in April and May. HSI data also show a positive correlation with GSI. The sex ratio among the female, male and sex undeterminated individuals was 0.51：0.20：0.29. The maximum body length was 55.9cm, and smallest body length was 9.5cm. I define the full maturity as appearance of the mushroom-like structure at the tips of the egg. P.cheni matured around 40cm. Gonad developed at 18.5cm body length, when minute eggs observable by naked eye are present. Lipids distribution in liver was significantly different in female before and after the reproduction period; lipid cells were more condense in the former period. Color of liver difference between the male and female P.cheni; male’s liver was more reddish. Therefore, before sex differentiation, I can identify the sex of an individual by the color of its liver.

目錄
章次 頁碼
壹、 緒言 1
貳、 材料與方法 3
參、 結果 9
肆、 討論 16
伍、 參考文獻 20
陸、 表 23
柒、 圖 29

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