DNA分子經由水力拉伸（Hydrodynamic Stretching）而產生形變的過程已經被廣泛研究。當DNA分子拉伸時，微體因不同流量、進口壓力及黏度下主流速度梯度分布，及流道壁邊的親水性及疏水性質造成的流體於壁邊產生滑動現象，導致不同的壁邊剪力流速度梯度分部，等相關實驗量測研究相當缺乏。故本實驗重要的目的即是利用微機電製程製造出適合DNA拉伸的微流系統及流動模式以共焦雷射掃描顯微鏡（Confocal Laser Scanning Microscope）作量測，再配合螢光微觀可視化的量測技術，計算拉伸的DNA分子於流道中的分部，對全域流動梯度加以作映像分析，將可以得到非常適合微流系統中流道尺寸及流體驅動力的重要設計資訊。
本實驗包括，使用微機電製程製造十字型的PDMS/Glass微流系統，藉由CLSM與DNA分子螢光可視化的實驗技術量測十字微流道延展流動之DNA分子於流道中拉伸之分布，及量測其拉伸長度、遷移速度以及應力鬆弛係數，希望藉由此對微流道中DNA分子動態及形變的演進及其性質有概廓的了解。

DNA molecules stretching and deformation in hydrodynamic flow field has been investigated for many years with various experimental and theoretical methods. This research was performed experimentally in hybridized microchannels made of poly(dimethylsiloxane)(PDMS) and glass. We will directly measure the hydrodynamic stretching DNA molecules in elongation flow by CLSM (Confocal Laser Scanning Microscope). The deformation of YOYO-labeled lambda phage DNA molecules in flows was visualized with CLSM. The relation between DNA contour length and relaxation time in different models of stretching flows was also be presented. Furthermore, the distribution of stretching DNA molecules in microchannels was derived to analyze and compared with the DNA migration velocity and stress relaxation modulus.

第一章 緒論
1-1　前言
1-2　背景與目的
1-3　文獻回顧

第二章 理論分析
2-1　DNA分子之基本性質
2-2　DNA分子之形變及水力拉伸性質
2-3　DNA分子於十字停滯流中之形變拉伸

第三章 實驗系統與設備
3-1 共焦雷射掃瞄顯微鏡量測系統
3-2　實驗製程設備

第四章 實驗量測方法及步驟
4-1　十字微流道製程
4-2　十字微流道封裝測試及環路架設.
4-3 共焦雷射掃描顯微鏡量測系統建立
4-4 DNA分子觀測及量測
4-5　微螢光質點追蹤
4-6　螢光可視化
4-7　影像分析

第五章 誤差分析.
第六章 結果與討論
6-1　DNA分子於十字流道中整體拉伸分布分析
6-2　DNA分子於轉角區及出口區拉伸分布分析
6-3　DNA分子拉伸演化追蹤分析
6-4　十字流道中DNA分子遷移速度與應力鬆弛分析

第七章 結論與建議
7-1　結論
7-2　建議及改進

參考文獻
附錄A 誤差分析

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