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博碩士論文 etd-0722104-062044 詳細資訊
Title page for etd-0722104-062044
論文名稱
Title
超快雷射與雷射掃描顯微術之應用:高頻光致電流及雙光子紫外螢光成像
The Applications of Ultrafast laser in Laser Scanning Microscopy:RFOBIC and Two Photon UV Fluorescence Microscopy
系所名稱
Department
畢業學年期
Year, semester
語文別
Language
學位類別
Degree
頁數
Number of pages
45
研究生
Author
指導教授
Advisor
召集委員
Convenor
口試委員
Advisory Committee
口試日期
Date of Exam
2004-07-07
繳交日期
Date of Submission
2004-07-22
關鍵字
Keywords
光致電流、氨基酸、高峰值功率、極寬頻寬、超快雷射、雙光子紫外螢光
high energy pulse, broad bandwidth, OBIC, two photon uv fluorescence, amino acid, ultrafast laser
統計
Statistics
本論文已被瀏覽 5676 次,被下載 5113
The thesis/dissertation has been browsed 5676 times, has been downloaded 5113 times.
中文摘要
在本研究中,充分利用超快雷射之極短脈衝和極高之瞬間功率分別應用在高頻光致電流及雙光子螢光上,由於超快雷射之超短脈衝和極寬的頻寬等特性,可產生大於1.8THz之頻寬,故可用來檢測高速元件;而利用腔內倍頻之光參數振盪器極高之瞬間功率及UVB短波長來取得紫外螢光訊號,並期待UVB的激發卻不受UVB破壞之影響。
Abstract
In this study, the characteristic properties of the ultrafast laser exhibit sufficiently in the application of RFOBIC and two-photon UV fluorescence. This laser can be used to measure photonic components with fast responding speed due to the ultrashort pulse and broad bandwidth which is RF bandwidths of greater than 1.8THz.
we have demonstrated the use of a frequency-doubled femtosecond optical parametric oscillator in generating two-photon excitation that is equivalent to ultraviolet(UV) light with wavelength less than 300 nm. This capability allows observation of some amino acids and enables excitation that is only possible with wavelength in UVB range(290 nm-320 nm)
目次 Table of Contents
中文/英文摘要
目 錄
表目錄
圖目錄

第一章 簡介

第二章 高頻光致電流顯微術
第一節 前言
第二節 光致電流與高頻光致電流
2.1 光致電流產生
2.2 高頻RF量測原理
2.3 載子產生
第三節 樣品介紹
3.1 光電二極體(photodiode)種類
3.2 二極體的響應速度
3.3 樣品photodiode
第四節 實驗架設與方法
4.1雷射光源
4.2共焦顯微系統
4.3成像系統及頻譜偵測
4.3.1 低噪音前置放大器(Low-noise preamplifier)
4.3.2 高頻半導體檢波整流器(RF diode detector)
4.3.3 頻譜分析儀
第五節 實驗結果與討論
5.1 光致電流(DCOBIC)
5.2 高頻光致電流(RFOBIC)
5.2.1 改變外加偏壓與RF影像之關係
5.2.2 改變外加偏壓與RF頻譜之關係
5.2.3 雷射光束聚焦在二極體不同位置上的頻譜之關係
5.3 其他PIN二極體實驗結果
5.3.1 二極體外型相似
5.3.2 不同之二極體結構
5.3.3 小面積之二極體
第六節 高頻光致電流顯微術結論

第三章 雙光子紫外螢光顯微術
第一節 前言
第二節 樣品介紹
2.1 氨基酸
2.2 樣品
第三節 實驗架設與方法
3.1雷射光源
3.2共焦掃描顯微系統
3.3 成像及光譜偵測
3.4 光參數振盪器
第四節 實驗結果與討論
4.1 氮化鎵之結果
4.2 氨基酸之紫外螢光
4.2.1 氨基酸之光譜
4.2.2 氨基酸之影像
第五節 雙光子紫外螢光顯微術之結論

第四章 總結與未來展望
參考文獻 References
[1.1]W. Denk, J. H. Stricker and W. W. Webb, ” Two-photon laser scanning fluorescence microscopy,” Science, Vol.248, pp. 73-76 (1990).
[1.2]J. Mertz, C. Xu and W. W. Webb, "Single-molecule detection by two-photon excited fluorescence", Opt. Lett., Vol.20, pp. 2532-2534 (1996).
[1.3]Xu. C and W. Denk,” Two- photon optical beam induced current imaging through the backside of integrated circuits”, Apply. Phys. Lett.,Vol74, p2578, 1997
[1.4]F.J. Kao, M.K. Huang and Y.S. Wang, “Two-photon optical beam induced vurrent imaging of indium gallium nitride blue light-emitting diodes”, Optics Letters, Vol.24, No.20, pp1407-1409, 1999
[1.5]G. D. Reid and K. Wynne, “Ultrafast Laser Technology and Spectroscopy, Encyclopedia of Analytical Chemistry”, pp13644-13670, 2000
[1.6]W. Denk, J. H. Stricker and W. W. Webb, ” Two-photon laser scanning fluorescence microscopy”, Science, Vol.248, pp. 73-76, 1990
[1.7]Catherine A. Royer, Biophysical Journal, Vol.68, pp. 1191-1195, 1995.
[1.8]Ignacy Gryczynski, Henryk Malak, Joseph R. Lalowicz, Biospectroscopy, Vol.3, pp. 97-101, 1996.
[1.9]OPO Basic Ring Manual, Coherent.

[2.1]T. Wilson and C.J.R. Sheppard, “Theory and Practice of Scanning Optical Microscopy”, Academic Press, London, 1984
[2.2]P.D. Pester and T. Wilson, “Photoluminescence and Optical Beam Induced Current Imaging of Defects”, SPIE, vol.1028 Scanning Imaging, 1988
[2.3]P.D. Pester and T. Wilson, “Time-dependent theory of optical-beam-induced current imaging of defects in semiconductors”, J. Appl. Phys., 64(3), pp1131-1135, 1988T.
[2.4]Wilson and E.M. McCabe, “Distribution of charge carriersgenerated in semiconductor by a focused convergent light beam”, J. Appl. Phys., pp2638-264259 (8), 1986
[2.5]Shinichi Takasu, “Application of OBIC/OBIRCH/OBHIC(Semiconductor Failure Analysis)”, Application & Research Center, JEOL Ltd
[2.6]T. Wilson and C.J.R. Sheppard, “Theory and Practice of Scanning Optical Microscopy”, Academic Press, London, 1984
[2.7]T. Wilson and E.M. McCabe, “Distribution of charge carriersgenerated in semiconductor by a focused convergent light beam”, J. Appl. Phys., pp2638-264259(8), 1986
[2.8]T. Wilson and E.M. McCabe, “Theory of optical beam induced current images of defects in semiconductors”, J. Appl. Phys. 61(1), pp191-195, 1987
[2.9]Adel S. Sedra and Kenneth C. Smith, Microelectronic Circuits, Fourth edition
[2.10]黃茂闊, “雙光子共焦顯微鏡與顯微光譜之應用:氮化鎵銦發光二極體的光致電流影像和顯微光譜”, 國立中山大學物理研究所碩士論文(2000)
[2.11]施勝志, ”超快雷射在顯微影像上的應用:高頻光致電流與二倍頻顯微技術”, 國立中山大學物理研究所碩士論文,2002
[2.12]F.J. Kao, J.C. Chen, S.C. Shih, A. Wei, S.L. Huang, T.S. Horng, and P. Török, “Optical beam induced current microscopy at DC and radio frequency”, Optics Communication 211, pp39-45, 2002
[2.13]E. Oran Brigham, The Fast Fourier transform, Prentice Hall Inc., 1974, Chap5
[2.14]HAMAMATSU, Photodiodes, 1995
[2.15]孫慶成, 光電概論, 1997
[2.16]Standford research system (SRS), SR560
[2.17]Low Noise PreamplifierHEWLETT PACKARD, 8473D Specification
[2.18]HEWLETT PACKARD, 8563E Specification and Characteristics

[3.1]W. Denk, J. H. Stricker and W. W. Webb, ” Two-photon laser scanning fluorescence microscopy,” Science, Vol.248, pp. 73-76 (1990).
[3.2]J. Mertz, C. Xu and W. W. Webb, "Single-molecule detection by two-photon excited fluorescence", Opt. Lett., Vol.20, pp. 2532-2534 (1996).
[3.3]G. Weber, and F.W.J. Teale, Biochem.J., Vol.65, pp. 476-482, 1957.
[3.4]Toshifumi, Yasuhiro, J. Chem. Phys., Vol.114, pp. 2867, 2001.
[3.5]M. Lasagna, E. Gratton, D. M. Jameson, and J. E. Brunet, “Apohorseradish Peroxidase Unfolding and Refolding: Intrinsic Tryptophan Fluorescence Studies”, Biophys J, Vol.76(1) , pp. 443-450 (1999).
[3.6]A. A. Rehms and P. R. Callis, “Two-photon fluorescence excitation spectra of aromatic amino acids”, Chemical Physics Letters, Vol.208, pp. 276-282 (1993).
[3.7]G. Weber, and F.W.J. Teale, “Ultraviolet fluorescence of aromatic amino acids”, Biochem. J., Vol.65, pp. 476-482 (1957).
[3.8]H. Lodish et al., “Molecular Cell Biology” , Media Connected, New York, 2000.
[3.9]J. Davies, and B. S. Littlewood, “Elementary Biochemistry-An Introduction the Chemistry of Living Cells”, Prentice-Hall Inc, New Jersey (1979).
[3.10]C. C. Wang and G. W. Racette, Appl. Phys. Lett., Vol.6, pp. 169(1965)
[3.11]J. A. Giordamaine and R. C. Miller, “Tunable coherence parametric oscillator in LiNbO3 at optical frequencies” , Phys. Rev. Lett., Vol.14, pp. 973-976, 1965
[3.12]D. C. Edelstein, E. S. Wachman, and C. L. Tang, “Broadly tunable high repetition rate femtosecond optical parametric oscillator”, Appl. Phys. Lett. Vol.54, pp. 1728-1730, 1989
[3.13]E. S. Wachman, D. C. Edelstein, and C. L. Tang, “Continuous-wave mode-locked and dispersion-compensated femtosecond optical parametric oscillator”, Opt. Lett., Vol.15, pp. 136-138, 1990
[3.14]W. S. Pelouch, P. E. Powers, C. L. Tang, “Ti:sapphire-pumped, high-repetition-rate femtosecond optical parametric oscillator,” Opt. Lett. 17, pp. 1070-1072(1992).
[3.15]G. Mak, Q. Fu, and H. M. van Driel, in Digest of Conference on Laser and Electra-Optics (Optical Society of America, Washington, D. C., 1992) paper CWD 1, pp. 236, G. Mak, Q. Fu, and H. M. van Driel, in Digest of Ultrafast Phenomena VIII (Ecole National Supperieure Techniqueet Advance, Paris, 1992) pp. 394.
[3.16]D. E. Spence, P. N. Kean, and W. Sibbett, “60-fsec pulse generation from a self-mode-locked Ti:sapphire laser”, Opt. Lett. 17, pp. 42-44(1991).
[3.17]Bob Proctor, and Frank W. Wise, “Quartz prism sequence for reduction of cubic phase in a mode-locked Ti:Al2O3 laser”, Opt. Lett. 17, pp. 1295-1297, 1992
[3.18]OPO Basic Ring Manual, Coherent.
[3.19]林敬仁, “以光參數震盪器對牙齒切片行諧頻顯微術觀測”, 國立中山大學物理研究所碩士論文, 2003
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