本篇論文報告使用一新穎技術「切片式螢光成像顯微術」研究ICN光解。切片式螢光成像顯微術結合顯微及切片兩種技術改善習知的螢光與離子成像的速度解析度。它是利用高功率雷射聚焦鏡將光解-偵測雷射聚焦於氣相前置物，產生光解並激發光解碎片，同時利用顯微物鏡視野深度有限的特性得到碎片的螢光小球切片，最後藉助CCD相機紀錄螢光切片的成像。
本實驗使用308 nm準分子雷射光解ICN分子產生CN碎片，並使用388.34 nm的聚焦偵測雷射激發CN碎片產生螢光。雖然實驗因為硬體上的限制無法得到預期的結果，但是未來經由硬體的改善相信切片式螢光成像顯微術會成為研究氣相化學動態學的另一種有力技術。

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摘要......................................................I
目錄....................................................III
圖目......................................................V
壹、簡介............................................1
一.以成像技巧研究化學動力學的回顧.........................1
1.離子成像法..............................................1
2.螢光成像技巧............................................3
二.切片式螢光成像顯微術原理...............................4
三.使用ICN作為光解系統的動機..............................5
貳、實驗步驟..............................................7
一.實驗系統...............................................7
1.顯微光學系統............................................7
2.雷射光源................................................7
3.CCD偵測系統.............................................8
4.時序控制................................................8
5.真空系統................................................9
二.實驗步驟..............................................10
1.光解與偵測雷射90˚夾角的實驗...........................10
2.觀察光解雷射焦點的實驗.................................11
3.光解與偵測雷射0˚夾角的實驗............................11
參、結果與討論...........................................12
1.CN 螢光的偵測..........................................12
2.光解雷射焦點的校正.....................................13
3.時序的調整.............................................14
4.0°切片式螢光成像的觀察.................................17
肆、結論.................................................19
伍、未來的發展...........................................20
陸、參考文獻.............................................67

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