直接電噴灑游離探針(Direct electrospray probe, DEP)是一種大氣壓力游離法，而它擁有許多優點，如減少樣品使用量，更換樣品快速，較不會產生交叉汙染之現象，不需以微量幫浦來推動樣品溶液等優點。本研究改良了直接電噴灑游離法的介面，因此可以使得噴灑時間延長到約1分鐘，得以供化學分析所需。而藉由穩定的噴灑可以結合主成分分析法(Principal component analysis, PCA) 的統計方式，如此一來更可以簡化所得結果並得到群聚的呈現。利用改良過的直接電噴灑游離法對於分析酒精飲料是相當適合的，因為其中所含酒精及大量的離子所以可以酒體直接進行分析而不需要前處理。本研究，探討了電壓、樣品體積、金屬片厚度及角度的參數以此獲得了最佳化條件為，電壓7.0 kV，樣品體積3 μL，金屬片厚度 0.1 mm，金屬片角度 25 角度。利用此介面來對各種酒類的區別，以市售13種的酒類為樣品進行區分。接著將同種類的酒來做樣品分析，包含：紅酒、白酒、啤酒，結果顯示對於相同原料，但是製作手法不同的酒類，也可以做出判別。我們更進一步模擬三種山寨酒的製作手法，來進行探討，包含兩類：以酒精水溶液稀釋樣品或混合兩種酒類；模仿高級酒混合劣質酒以及目前較常見以香料與酒精水溶液製成的溶液添加到原料酒，而分析結果可以得到依不同比例混合而得的規律以及有其趨勢的變化。此外我們以葡萄及市售葡萄汁添加市售紅酒酵母菌來模擬發酵過程，共進行七天的採樣，對發酵過程進行即時的偵測。此方法對於發酵的品質以及過程可以達到監控的目的。最後我們將酒樣放置在開放的環境下，每小時分析一次，共五個小時進行酒類品質的偵測。雖然其變化程度很小，但是本研究還是可以做出區別。未來，利用本研究之方法，可對於劣質酒與未知酒來做快速篩選，以及可進行酒發酵的監控來確保品質等應用。

A novel ambient ionization method which is so-called direct electrospray probe (DEP) has been developed in this study. This method provides rapid and high throughput analysis due to its advantages such as no sample pretreatment, low sample consumption, easily to change sample. In the study, we improved the interface of direct electrospray probe, and extend spray time to 1 minute for chemical analysis. Because of extending spray time, the method could be combined with principal component analysis (PCA), which is one kind of statistical method. Therefore, we applied the method for determination of alcoholic beverage which contains a lot of ions and alcohol. First, the conditions such as applying voltage, sample volume and shape of probe have been modified. The probes we used in the study were made by used metal pieces. These metal pieces with different thickness and tip angle to be test. Then, we found the optimize conditions as: voltage 7.0 kV, sample volume 3 μL, thicknss of metal piece 0.1 mm, angle of metal piece 25 degree. Classification of 14 brands of wines which were purchased from supermarket was determined and analyzed by the method. Otherwise, we used the method to distinguish real wines from hedge wines we simulated. The hedge wines were prepared by adding flavor in alcohol solutions. Furthermore, we also used this method to observe fermentation process of wine production. Finally, deterioration of wine under exposure in air was also determined by the method. The results of the study demonstrated that the method we developed can be applied to distinguish hedge wines from real wines. And, it can used to monitor the fermentation process of wine production for quality control.

論文審定書………………………………………………………………………...…….I
謝誌……………………………………………………………………………………...II
中文摘要………………………………………………………………………………III
英文摘要……………………………………………………………………………….IV
目錄………………………………………………………………………………..……V
圖目錄…………………………………………………………………………………VII
表目錄…………………………………………………………………………………XI
壹 緒論…………………………………………..………………………………….1
1.1前言………………………………………………………………………….… 1
1.2大氣壓力質譜法…………………………………………………………….….2
1.3電噴灑游離法…………………………………………………………………..3
1.4以電噴灑游離法為基礎的大氣壓力游離法……………………………….….5
1.5直接電噴灑游離探針………………………………….………………………8
1.6主成分分析……………………………………………………………………11
1.7酒精飲料……………………………………………………………………….15
1.8 論文目標……………………………………………………………………….17
貳 實驗..…………………………………………………………………………18
2.1 實驗裝置……………………………………………………………………..18
2.2實驗藥品與樣品………………………………………………………………18
2.3樣品配置………………………………………………………………………20
2.4軟體參數設定…………………………………………………………………21
參 結果與討論…….…………………………………………………………..23
3.1實驗步驟……………………………………………………………………………23
3.2介面參數探討…………………………………...………………………………….26
3.3不同種類與相似相同種類酒精飲料的區分………………….……..……….……32
3.4劣質酒的區分………………………………………………………………………40
3.5模擬葡萄酒發酵並對其監測………………………………………………..…….54
3.6將酒樣進行揮發並對其做監測…………………………………..……………….60
肆 結論………………………….………………………………………………66
伍 參考文獻…………………………………………….………………………...67
陸 附錄…………………………………………….…………………………….72

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