基板支撐的脂質雙層膜(supported lipid bilayers, SLBs)是模擬生物細胞膜的模型之一。SLBs的形成受到許多因素的影響，如基板、溶液的離子強度、溫度、pH值等。本研究利用原子力顯微鏡(AFM)觀察L-α-lysophosphatidylcholine, egg PC所組成的微脂粒(liposome)於不同種類及濃度的離子溶液中在mica基板上的成膜現象，實驗結果說明二價離子加入微脂粒溶液中有助於SLB的形成。
不同脂質分子的相轉移溫度(phase transition temperature)各有不同，影響脂質分子間的排列。本研究利用AFM輔以溫度控制裝置對dioleoylphosphatidylcholine / dipalmitoylphosphatidylCholine(DOPC/ DPPC)混合雙層膜觀察其相轉移變化，發現溫度對脂質區塊的形成有很大的影響。
界面活性劑與生物膜之間的溶解行為為生物技術上重要之應用。本實驗製備 DOPC/DPPC及dioleoylphosphatidylcholine/ sphingomyelin (DOPC/SpM)脂質雙層膜與界面活性劑TX-100作用，觀察TX-100分子與膜的溶解行為。發現不同濃度的TX-100與DOPC/DPPC雙層膜有不同的溶解方式。另外，SpM區塊在低溫環境下能夠不被TX-100所溶解。

Supported lipid bilayers (SLBs) are unique model systems for biological membranes. SLBs can be formed by fusing liposomes on solid substrates, which can be characterized by a variety of surface analytical techniques, such as Atomic Force Microscopy (AFM), X-ray diffraction, Quartz Crystal Microbalance (QCM), etc. In this study we used AFM to investigate the dynamic process of the formation of SLBs from liposomes in solutions containing metal ions and phase separation between different lipids as a function of temperature. Divalent cations, Ni2+ in particular, was found to be critical to the deposition of bilayers.
Lipid rafts are plasma membrane microdomains rich in sphingolipid and cholesterol forming a liquid ordered phase surrounded by a liquid disordered phase. Lipid rafts are insoluble in cold non-ionic detergents, also called Detergent Resistant Membranes (DRMs). The interaction behaviors between detergent (Triton X-100) and mixed bilayers (DOPC/DPPC and DOPC/SpM) were studied by AFM. The way lipid bilayers were solubilized by Triton X-100 was quite different below and above its critical micelle concentration (CMC), and the SpM domains were found to be resistant to detergent extraction in the cold.

目 錄
摘 要 i
Abstract ii
目 錄 iii
圖目錄 vi
表目錄 ix
第一章 緒論 1
1-1 前言 1
1-2 磷脂質與脂雙層 3
1-3 脂筏 7
1-4 界面活性劑 9
1-5 微脂粒 11
1-6 模擬模型 13
1-6-1 黑脂膜(Black lipid membranes,BLMs) 13
1-6-2 基板支撐之脂質雙層膜(Supported lipid bilayers, SLBs) 14
1-6-3 混合雙層膜(Hybrid bilayer membranes, HBMs) 16
1-7 實驗動機 18
第二章 儀器與實驗 19
2-1 原子力顯微鏡 (Atomic Force microscope, AFM) 19
2-1-1 AFM原理 19
2-1-2 AFM之掃描模式 21
2-1-3 AFM數據分析 24
2-2 擠壓器 (Mini-Extruder) 29
2-3 粒徑分析儀 (Dynamic Light Scattering) 30
2-4 界面電位儀 31
2-5 實驗材料 33
2-6 製備微脂粒 35
2-7 SLB於mica上形成之動態過程 36
2-8 控制溫度觀察混合雙層膜之相轉變行為 37
2-9 混合雙層膜與界面活性劑之作用 38
2-9-1 DOPC/SpM與界面活性劑 38
2-9-2 DOPC/DPPC與界面活性劑 39
第三章 實驗結果與討論 40
3-1 微脂粒粒徑分析 40
3-2 脂雙層高度預測 42
3-3 離子溶液影響SLB形成 44
3-3-1 離子強度 44
3-3-2 離子濃度 46
3-3-3 離子種類 48
3-4 混合雙層膜之相轉變行為 51
3-5 界面活性劑對溶解雙層膜之行為機制 55
3-5-1 以0.12mM TX-100溶解脂雙層(<CMC) 56
3-5-2 以0.48mM TX-100溶解脂雙層(>CMC) 58
3-5-3 以10% TX-100溶解脂雙層(>>CMC) 59
3-6 TX-100與DOPC/DPPC脂雙層作用之結果討論 60
3-7 界面活性劑與抗洗滌劑膜(DRMs) 62
3-7-1 室溫下TX-100與DRMs之作用 62
3-7-2 低溫下TX-100與DRMs之作用 63
第四章 結論 66
參考文獻 68

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