中文摘要
以單細胞電泳法 (single cell gel electrophoresis ； Comet assay) 評估，Tannic acid (TA) 及其水解產物，如：Gallic acid (GA) 、Propyl gallate (PA) 及Ellagic acid (EA) 抑制Trp-P-2、PhIP (食品致突變物) 及H2O2對人類淋巴球DNA之傷害。在濃度為0.1-100 μg/ml時，並無發現TA及其水解產物對淋巴球具有細胞毒性。只有在100 μg/ml時，TA及GA造成DNA些微的損傷，而PA及EA則不會造成DNA的損傷。TA及其水解產物於0.1-10 μg/ml的濃度時，具有降低Trp-P-2、PhIP及H2O2對DNA之傷害。本實驗亦探討TA及其水解產物是否造成人類淋巴球DNA pyrimidine base及purine base的傷害，當TA及其水解產物於10 μg/ml的濃度時，TA、GA及PA具有保護purine base的效果，而EA則呈現具有保護purine及pyrimidine base的雙重效果。結果指出，TA及其水解產物可以降低Trp-P-2、PhIP及H2O2對人類淋巴球DNA之傷害，並具有保護DNA base之特性。

【關鍵字】：單細胞電泳法; Tannic acid; 人類淋巴球; H2O2

Abstract
The effect of tannic acid (TA), gallic acid (GA), propyl gallate (PA) and ellagic acid (EA) on DNA damage in human lymphocytes induced by food mutagens, 3-amino-1-methyl-5 H-pyrido (4,3-b)indole (Trp-P-2) and 2-amino-1-methyl-6-phenylimadazo (4,5-b)pyridine (PhIP), and/or H2O2 was evaluated using single-cell electrophoresis (comet assay). The toxicity of these tested compounds on lymphocytes was not found. These compounds did not cause DNA damage at lower concentration of 0.1-10 µg/ml. At a concentration of 100 µg/ml, TA and GA exhibited slight DNA damage, whereas PA and EA showed no DNA damage. TA and its related compounds decreased the DNA damage induced by Trp-P-2 or PhIP at a concentration of 0.1-10 µg/ml. Moreover, the inhibition of H2O2-induced DNA damage increased with increasing concentrations up to 10 µg/ml. DNA repair enzymes, endonuclease III (Endo III) and formamidopyrimidine-DNA glycoslase (FPG) were used to examine the levels of oxidised pyrimidines and purines in DNA damage induced by H2O2, respectively. All the compounds at 10 µg/ml can reduce the level of FPG sensitive sites. However, only EA inhibited the formation of EndoIII sensitive sites. The results indicate that these compounds can enhance lymphocyte resistance towards DNA damage induced by food mutagens or H2O2.
Keywords: Tannic acid; Human lymphocyte; Comet assay; Hydrogen peroxide

目 錄

中文摘要………………………………………………………………Ⅰ
英文摘要………………………………………………………………Ⅱ
前言…….………………………………………………………………1
材料與方法……………………………………………………………12
結果與討論……………………………………………………………19
結論……………………………………………………………………31
圖表……………………………………………………………………32
附錄……………………………………………………………………47
參考文獻………………………………………………………………51

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