含γ內酯之倍半萜類化合物是一種具有生物活性的化合物，而台灣澤蘭在過去的研究中曾經發現過此類化合物。因此以花蓮澤蘭作為研究的材料。台灣東部沿岸採集之花蓮澤蘭 (Eupatorium hualienense C. H. Ou, S. W. Chung & C.-I Peng) 為一台灣特有種植物。於，以甲醇丙酮之混合液萃取後，利用色層分析的方法分離出六個成分,分別為五個新天然化合物，命名為eupahualins A-E（1~5）及一個已知化合物eupasimplicin B（6）。這些化合物的結構鑑定為利用一維及二維核磁共振磁譜、紅外光譜、紫外光譜及質譜等方法完成。
Eupahualin A（1）在C-10上接有一個醛基，C-8接有一個a,b-unsaturated acyl的取代基。Eupahualins B、C（2、3） 在C-10
上同樣接有一個醛基，eupahualin B（2）與eupahualin A（1）之不同處在於eupahualin A（1）之C-4，C-5雙鍵為E-form，而eupahualin B（2）為Z-form。Eupahualin C（3）與eupahualin A（1）之不同處在於eupahualin A（1）的C-4'，C-5'依序為CH3與CH2OH，eupahualin C （3）則是相反。Eupahualin D（4）在C-10上接有exocyclic雙鍵，C-1及C-3上分別多了OH基及OAc基的取代。Eupahualin E（5）與eupahualin A（1）之不同處在於在Eupahualin E（5）之C-10上接有酸基。

Sesquiterpene lactones contain α,β-unsaturated-γ-lactone as a major structural feature, which in recent studies have been shown to be associ-
ated with anti-tumor, cytotoxic, anti-microbial and phytotoxic activities. Previous researchers isolated sesquiterpene lactones from Eupatorium formosanum Hay. Thus, we studied Eupatorium hualienense, a unique speces in Taiwan that grows near the eastern coast. Five new sesquiter-
pene lactones of the germacranolide type, eupahualins A-E (1-5) along with the known lactone, eupasimplicin B (6). Their structures were determined by 1D-NMR(1H-NMR,13C-NMR) and 2D-NMR(COSY、NOESY、HMQC、HMBC).
Eupahualin A (1) exhibits an aldehyde at C-10 and an a,b-unsatura
ted acyl group at C-8. Eupahualin B (2) also has an aldehyde at C-10. The Z-form of C-4, C-5 double bond in eupahualin B (2) is the main difference from the trans-form C-4, C-5 double bond in eupahualin A (1). The difference of eupahualin C (3) and eupahualin A (1) lies in structure of a ester group at C-8. The methyl at C-4' in eupahualin A (1) was changed into hydroxy methyl while the hydroxy methyl of eupahualin A (1) was changed to methyl in eupahualin C (3). Eupahualin D (4) shows exocyclic double bond at C-10, OH at C-1 and OAc at C-3. An carboxylic group (COOH) at C-10 in eupahualin E (5) is the only difference from which in eupahualin A (1).

壹 緒論 7
（一）前言 7
（二）相關研究之文獻回顧 9
（三）研究動機及目標 12
貳 材料與方法 13
（一）材料 13
（二）分離與純化 14
（三）衍生物製備 18
參 結果與討論 19
（一）結構解析 20
一 化合物1, Eupahualin A分子結構之鑑定 20
二 化合物2, Eupahualin B分子結構之鑑定 26
三 化合物3, Eupahualin C分子結構之鑑定 31
四 化合物4, Eupahualin D分子結構之鑑定 34
伍 化合物5, Eupahualin E分子結構之鑑定 39
六 化合物6 , Eupasimplicin B 分子結構之鑑定 42
（二）活性測試 44
（三）討論 45
參考文獻 46
附錄 各成份的光譜資料 48

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