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論文名稱 Title |
GN51傘軟珊瑚化學成分及癌細胞毒殺活性之研究
Chemical Constituents and Cytotoxicity of Formosan Soft Coral Xenia sp. (GN51) |
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系所名稱 Department |
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畢業學年期 Year, semester |
語文別 Language |
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學位類別 Degree |
頁數 Number of pages |
127 |
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研究生 Author |
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指導教授 Advisor |
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召集委員 Convenor |
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口試委員 Advisory Committee |
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口試日期 Date of Exam |
2003-07-14 |
繳交日期 Date of Submission |
2003-07-29 |
關鍵字 Keywords |
傘軟珊瑚 Xenia |
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統計 Statistics |
本論文已被瀏覽 5701 次,被下載 1430 次 The thesis/dissertation has been browsed 5701 times, has been downloaded 1430 times. |
中文摘要 |
綠島產 (Xenia sp.) (GN51)傘軟珊瑚之有機層萃取物經層析分離後得到12個化合物,分別為isoxeniolide-A (1), xeniolide-B (2), 4b -hydroxgermacra-1(10),5-diene (3), parahigginone (4), GN51-118 (5), GN51-119 (6), floride A (7), xenione (8), 13-epi-9-desacetyl-xenicin (9), xenitorin C (10), xenitorin A (11), xenitorin B (12)。其中GN51-118 (5)為新骨架的化合物, GN51-119 (6)為新化合物。 上述化合物經癌細胞毒殺活性測試後,xeniolide-B (2), xenione (8), 13-epi-9-desacetyl-xenicin (9)對P-388、HT-29細胞株均有顯著的抑制作用,化合物5對P-388細胞株有抑制作用。 |
Abstract |
Organisms of Formosan soft coral Xenia sp. (GN51) (collected at green island off Taiwan) were freeze-dried to give 600 g of a solid, which was extracted with CH2Cl2 and CH2Cl2/MeOH (1:1). After removal of solvent in vacuo, the residue was chromatographed over Sical gel 60 separation resulated in the isolation of two new diterpenes, GN51-118 (5) (new carbon skeleton) and GN51-119 (6), five known diterpenes isoxeniolide-A (1), xeniolide-B (2), floride A (7), xenione (8), 13-epi-9-desacetyl-xenicin (9) as well as five sesquiterpenes 4b- hydroxgermacra-1(10),5-diene (3), parahigginone (4), xenitorin C (10), xenitorin A (11)and xenitorin B (12)。 |
目次 Table of Contents |
目錄 頁次 壹、 緒論 1 一、前言 1 二、Xenia屬軟珊瑚之天然物化學研究回顧 2~23 三、研究動機及目的 24 貳、 GN51傘軟珊瑚化學成分之研究 一、材料採集與鑑定 25 二、GN51傘軟珊瑚之分類地位 25 三、GN51傘軟珊瑚之萃取與分離 26~27 四、GN51傘軟珊瑚化學成分之分離純化 28 (1) GN51-181之分離純化 28~30 (2) GN51-183之分離純化 31~33 (3) GN51-47之分離純化 34~36 (4) GN51-49之分離純化 37~39 (5) GN51-118之分離純化 40~42 (6) GN51-119之分離純化 43~45 (7) GN51-120之分離純化 46~48 (8) GN51-138之分離純化 49~51 (9) GN51-139之分離純化 52~54 (10) GN51-121之分離純化 55~57 (11) GN51-122之分離純化 58~60 (12) GN51-123之分離純化 61~63 五、GN51傘軟珊瑚化學成分之結構解析 (1) GN51-181之結構解析 64~68 (2) GN51-183之結構解析 75~78 (3) GN51-47之結構解析 85~88 (4) GN51-49之結構解析 95~99 (5) GN51-118之結構解析 108~113 (6) GN51-119之結構解析 123~128 (7) GN51-120之結構解析 135~136 (8) GN51-138之結構解析 139~142 (9) GN51-139之結構解析 149~153 (10) GN51-121之結構解析 160 (11) GN51-122之結構解析 163 (12) GN51-123之結構解析 166 参、 GN51傘軟珊瑚所分出化合物之細胞毒殺活性 169 肆、 結論 170 伍、 參考文獻 171~176 陸、 附錄 一、 儀器及材料 177~178 二、 癌細胞毒殺活性測試之分析方法 179 三、 MTT 分析原理 180 |
參考文獻 References |
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