螢光染料分子可因環境或分子聚集的效果而影響其發光性質，了解這些分子聚集效果及對其螢光之影響可以應用於其光電元件之應用。本論文中，我們研究高濃度溶液狀態下DiI_C18染料分子，藉著溶劑及溫度等參數來控制其溶解度，改變螢光分子之聚集，來探討其對螢光光譜的影響。我們分別使用水(water)、甲醇(methanol)、乙二醇(ethylene glycol)三種溶劑來控制分子聚集效果。實驗中，我們觀察到螢光光譜在不同溶劑中的光譜變化，特別是在甲醇(methanol)與水(water)的混合溶液中，隨著分子濃度的增加，分子之間的聚集效果造成吸收光譜的變化。除此之外，在高濃度溶液中，我們觀察到分子聚集之螢光光譜隨著激發光強度之光譜變化，顯示分子由單體結構聚集成聚合體結構。此外，經過約一天後，分子會排列成另一個幾何結構的聚合體。
在高濃度溶液中，染料分子會聚集成平行結構的聚合體和頭接尾結構的聚合體。平行結構的聚合體在較低之聚集能階狀態為兩分子電偶極矩反相之組合，不僅所得為較低之吸收強度，則具有較長的螢光生命期。而頭接尾結構的聚合體在較低之聚集能階狀態為兩分子電偶極矩同相之組合，所得為較高之吸收強度，則具有較短的螢光生命期。在這二種分子聚合體中，平行結構的聚合體比頭接尾結構的聚合體的光穩定性差。

Luminescent properties of the dye molecules can be influenced by the environments as well as intermolecular interactions. Suitable control the aggregation can be useful for optoelectronic device applications. In this thesis, we investigate the spectroscopic properties of dye molecule, DiI_C18, in solutions. Solvents and concentrations are used to control the degree of aggregation. Absorption and emission are used to probe the properties. We use water, methanol, and ethylene glycol as key solvents to control the aggregation effects, especially the methanol solution mixing with water.
As the concentration increased, the change of the absorption spectra are observed, which is caused by the aggregation between molecules. Two types of aggregations: head-to-tail structure and parallel structure, are proposed. Head-to-tail structure behaves red-shifted spectrum, and a corresponding shorter decay lifetime. On the contrary, parallel structure aggregation exhibits blue-shifted absorption spectrum and a longer fluorescence lifetime. Both structures are observed at different period after mixing. Only monomer exists in the methanol solution, even at high concentration. However, after missing with water, parallel structure aggregates are formed. Head-to-tail structure aggregates are formed after a much longer period, usually several hours after the mixing.
These aggregates not only have different photo-physical properties. After high light intensity illumination, parallel structures are easier to turn into non-fluorescent structures, while head-to-tail structures last a longer period. Monomer structures exhibit the longest period. This provides additional evidence for the formation of different structures in the mixing solutions.

中文摘要 I
Abstract III
致謝 IV
目錄 V
圖目錄 VIII
表目錄 XI
第一章 簡介 1
1.1、 背景 1
1.2、 動機 4
第二章 原理 5
2.1、螢光簡介 5
2-1-1 螢光的機制 5
2-1-2 螢光量子效率(luminescence quantum yield) 9
2.1.3 螢光生命期 10
2.2、螢光量測 15
2.2.1 近場光學顯微鏡(NSOM) 15
2.2.2 共焦顯微鏡(confocal microscope) 15
2.2.3 廣場顯微鏡(wide-field microscope) 16
2.3、樣品介紹 17
2.3.1 染料分子 17
2.3.2 分子聚集狀態 19
第三章 實驗系統架構 22
3.1、螢光實驗儀器架構 22
3.1.1、實驗步驟 22
3.1.2、螢光光譜實驗架構 24
3.1.3、螢光生命期實驗架構 26
第四章 實驗結果與討論 27
4.1、樣品在不同溶劑的螢光表現 27
4.2、在甲醇與水的混合溶液中，不同濃度染料分子(DiI_C18)的吸收光譜 30
4.2.1、在甲醇與水的混合溶液中，不同濃度染料分子(DiI_C18)的螢光光譜 31
4.2.2、在甲醇與水的混合溶液中，改變時間染料分子(DiI_C18)的吸收和螢光光譜 32
4.2.3、在甲醇與水的混合溶液中，改變雷射強度染料分子(DiI_C18)的螢光光譜 35
4.3、在甲醇與水的混合溶液中，染料分子(DiI_C18)的螢光生命期 40
4.3.1、在甲醇與水的混合溶液中，新鮮溶液的螢光生命期 40
4.3.2、在甲醇與水的混合溶液中，過一天後溶液的螢光生命期 43
4.4、在樣品薄膜上，染料分子(DiI_C18) 的螢光光譜 45
4.4.1、在樣品薄膜上，改變雷射強度染料分子(DiI_C18) 的螢光光譜 46
4.5、在樣品薄膜上，染料分子(DiI_C18) 的螢光生命期 49
4.5.1、在樣品薄膜上，低雷射強度的染料分子(DiI_C18) 螢光生命期 49
4.5.2、在樣品薄膜上，高雷射強度的染料分子(DiI_C18) 螢光生命期 51
第五章 結論 53
第六章 參考資料 57

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