本研究主要的目的是在探討裂片石蓴 (Ulva fasciata Delile) 在過量銅環境下藻體內銅累積情形以及對藻體碳、氮與磷的影響。不同銅濃度處理下，U. fasciata的每日比生長速率 (daily specific growth rate) 與總磷含量隨著銅濃度的增加而減少，而藻體總銅含量、細胞壁銅含量與細胞內銅含量則隨銅濃度增加而增加，由每日比生長速率與外加銅濃度之關係發現U. fasciata忍受銅濃度的上限為100 uM。以不同濃度的銅處理4日後，U. fasciata的Pi 吸收顯著地受到抑制，同時由藻體不同磷的成分變化知藻體總磷減少含量主要是因為總可溶性磷 (total soluble phosphorus, TSP) 與可溶性磷 (soluble reactive phosphorus, SRP) 含量的減少；所以，銅造成總磷含量下降原因之一可能是因銅抑制U. fasciata磷之吸收以致細胞內可利用之可溶性磷含量不足。在100 uM 銅處理之時間序列變化實驗，每日比生長速率及藻體總磷含量在第4日後顯著下降而TTC還原能力則隨時間而降低，藻體總銅含量在處理初期即增為對照組 (0 uM) 的10倍後維持不變，第3日始又增加。由試驗結果可以找出每日比生長速率以及藻體總磷含量的IC50 (concentration of 50 % inhibition)，每日比生長速率的IC50在ASW中以及藻體內分別為9.8 uM 以及0.76 mg．g-1 DW ，而藻體總磷含量之IC50則分別為37.8 uM 以及1.44 mg．g-1 DW。銅會抑制U. fasciata的生長，並且累積在藻體以及細胞壁，藻體累積的銅會影響其生理、代謝作用，抑制Pi 吸收，使得可以利用的磷減少，造成藻體的總磷含量下降。

The effects of copper on growth and phosphorus (P) utilization were investigated in the marine chlorophyte Ulva fasciata Delile. Both the daily specific growth rate and tissue P contents decreased as increasing CuSO4 concentrations, while the contents of total tissue, intracellular and cell-wall Cu increased. Based on the relationship between daily specific growth rate and external CuSO4 concentrations, the upper limit of U. fasciata is 100 uM CuSO4. After 4-day exposure to varying CuSO4 concentrations, Pi uptake was inhibited. Analysis of P fraction in U. fasciata exposed to 100 uM CuSO4 shows that the Cu-induced decline in total tissue P contents is mainly due to a decrease in both soluble reactive P (i.e. Pi). Exposure to 100 uM of Cu caused the accumulation of total tissue Cu contents to a plateau and then rose again at day 3, and tissue P contents and daily specific growth rate decreased at day 4. IC50 (concentration of 50 % inhibition) of daily specific growth rate and tissue P contents are 9.8 and 37.8 uM of Cu concentration in the medium, respectively, and 0.76 and 1.44 mg．g-1 DW for total tissue Cu contents, respectively. Overall, Cu causes Cu accumulation in intracellular space and cell wall and decrease of growth and P contents of U. fasciata partly via Pi uptake inhibition.

一、前言
二、材料與方法
﹙一﹚材料栽培及處理
試驗一 不同銅濃度處理之外表型態與生理反應試驗
試驗二 銅處理之時間序列試驗
試驗三 銅處理與Pi 吸收之關係
﹙二﹚分析方法
1. 每日比生長速率 (daily specific growth rate) 測定
2. 藻體總碳含量測定
3. 藻體總氮及總磷含量測定
4. 藻體總溶解磷、溶解可利用磷與固體磷含量測定
5. 藻體銅含量測定
6. 水樣無機磷 (DIP) 含量測定
7. TTC還原能力
8. 統計分析
三、結果
﹙一﹚銅處理下裂片石蓴外表形態、生長與內部組成之變化
1. 外表型態與生長
2. 銅含量分析
3. C、N、P、TSP與SRP含量分析
﹙二﹚100 uM銅處理裂片石蓴之時間序列變化
1. 每日比生長率與TTC還原能力
2. 銅含量分析
3. C、N、P、TSP與SRP含量分析
﹙三﹚銅處理與Pi 吸收之關係
四、討論與結論
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