PPAR (peroxisome proliferator activated receptors)為調控脂質代謝相關酵素基因之轉錄因子，與PPRE (peroxisome proliferator response element)作用可促進或抑制基因表現。本研究以建構於肝癌細胞(Huh-7)之PPRE-Luciferase 偵測不同藻類及海羊齒乙酸乙酯萃粗取物對PPAR/PPRE 之影響，並以(3-(4,5-imethylthiazol-2-yl)-2,5- diphenyltetrazolium bromide, MTT )進行細胞活性分析以區別細胞毒性之差異。大型藻類，龍鬚菜(Gracilaria coronopifolia J. Agardh)粗萃取物具有促進PPRE 效應且沒有細胞毒性。石花菜之細翼枝菜(Pterocladiella capillacea Santelices)與日本石花菜(Gelidium japonicum Okamura)及麒麟菜(Euchema sp.)粗萃取物具有促進PPRE 效應但卻有細胞毒性。安曼司石花菜(Gelidium amansii Lamouroux)粗萃物沒有PPRE 效應且沒有細胞毒性。紫菜(Porphyra sp.)粗萃取液抑制PPRE 效應且有細胞毒性。微細藻類，南寶公司提供之冷凍乾燥螺旋藻類 (spirulina sp.)粉粗萃物具促進PPRE 效應，且不具細胞毒性。由輔英科技大學周德珍老師提供之小球藻粗萃物會抑制PPRE 效應，卻不具細胞毒性。海洋齒(Echinodermata sp.) 粗萃物具有殺死蚊子幼蟲孑孓(toxicity)及防止蚊子接近(repellent)效果，但沒有PPRE 效應且沒有細胞毒性。本研究指出不同藻類及海洋齒具有不同之PPRE 效應及細胞毒性，龍鬚菜及螺旋藻具有PPRE調控之開發價值，海洋齒則有有綠色農藥之潛力。

PPAR (peroxisome proliferator activated receptors) is a transcription factor that regulates activity and transcription of enzymes of lipid metabolism and thought PPAR-PPRE (peroxisome proliferator activated receptors – peroxisome prolierator response element) interaction. Cell toxicity was also tested by (3-(4,5-dimethylthiaz ol-2-yl)-2,5-diphenyltetrazolium bromide (MTT assay)). The effects of ethyl acetate (EA) extracts of algae and Echinodermata sp. on PPRE-luciferase activity in Huh-7 cell and cell cytotoxicity were conducted. EA extracts of Gracilaria coronopifolia can induce PPRE-luciferase activity without cytotoxity. EA extracts of Pterocladiella capillacea, Gelidium japonicum, and Euchema sp. showed PPRE-luciferase activity and cytotoxicity. Gelidium amansii and Porphyra sp. did not show PPRE-Luciferase activity but had cyotoxicity. EA extracts of Spirulina sp. had PPRE-Luciferase activity but no cytotoxicity, while that of Chlorella sorokiniana inhibited PPRE-Luciferase activity but did not show cytotoxicity. EA extracts of Echinodermata sp. can kill larva and repellent of mosquito, but had no PPRE-Luciferase activity and cytotoxicity. These results demonstrated that the effects of EA extracts on PPRE-luciferase activity are different between algae. EA extract of Gracilaria and Spirulina showed PPRE regulation activity and Echinodermata sp. EA extract have the potential to become green chemical.

謝辭----------------------------------------------------------------i
中文摘要----------------------------------------------------------ii
英文摘要----------------------------------------------------------iii
目錄----------------------------------------------------------------iv
圖目錄-------------------------------------------------------------v
表目錄-------------------------------------------------------------ix
附錄目錄----------------------------------------------------------x
縮寫字對照-------------------------------------------------------xi
一、前言----------------------------------------------------------1
二、實驗進行之策略-------------------------------------------7
三、實驗流程圖-------------------------------------------------7
四、材料與方法-------------------------------------------------8
五、結果----------------------------------------------------------17
六、討論----------------------------------------------------------21
七、參考文獻----------------------------------------------------27
八、附錄----------------------------------------------------------53

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