Title page for etd-0802111-140814


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URN etd-0802111-140814
Author Yu-Wen Huang
Author's Email Address No Public.
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Department Institute of Biomedical Sciences
Year 2010
Semester 2
Degree Master
Type of Document
Language zh-TW.Big5 Chinese
Title Protein Oxidation and Inflammation induction in Hemodialysis patients
Date of Defense 2011-07-18
Page Count 88
Keyword
  • inflammatory
  • HEK293
  • ESRD
  • oxidative stress
  • Hemodialysis
  • Abstract Chronic inflammation is considered strongly influence the morbidity and mortality of patients with end-stage renal disease (ESRD) through its multiple pathogenic roles, in association with oxidative stress, accelerated aging, endothelial  dysfunction  and  atherosclerosis,  malnutrition,  dialysis-related amyloidosis, anaemia, and immune dysfunction . Hemodialysis (HD) is widely used for kidney failure patients,it is a method for removing waste products such as creatinine and urea. However, at present it is well known the course  of  hemodialysis  can  create  obvious  inflammation condition  and oxidation  pressure. The oxidation stress of HD can arise from the osmosis pressure and oxidative environment of dialysis tube.  The oxidative stresses will finally modify proteins which turn out initiate the short term and long term complications related to renal diseases of HD patients. We identified oxidated proteins in the hemodialysis tube of 16 HD patients. The protein oxidation level was determined by Oxyblot assay.  The oxidation proteins were further identified by LC/MS detection.  Many serum proteins were detected to be oxidized including albumin, apoA, immunoglobin,beta-globin, hemoglobin, etc. It has been well documented that albumin is quite vulnerable to ROS and elevated levels of carbonyl groups of albumin have been reported in plasma of dialysis patients. Inflammatory effects were further tested.  The oxidation proteins of HD patients induce pro-inflammatory factor TNF-alpha expression of HEK293T and HEK293 cells. These results indicate HD induce protein oxidation, and inflammatory response which may responsible for complications of End-stage kidney disease (ESRD).
    Keywords:Hemodialysis, oxidative stress, ESRD, HEK293, inflammatory
    Advisory Committee
  • Kuang-Hung Cheng - chair
  • Hsueh-Wei Chang - co-chair
  • Hung-Wen Huang - advisor
  • Files
  • etd-0802111-140814.pdf
  • Indicate in-campus at 1 year and off-campus access at 99 year.
    Date of Submission 2011-08-02

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