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博碩士論文 etd-0807101-171345 詳細資訊
Title page for etd-0807101-171345
論文名稱
Title
綠島海域花環肉質軟珊瑚Sarcophyton glaucum所含天然物之研究
Studies on the Metabolites of the Formosan Soft Coral Sarcophyton glaucum
系所名稱
Department
畢業學年期
Year, semester
語文別
Language
學位類別
Degree
頁數
Number of pages
70
研究生
Author
指導教授
Advisor
召集委員
Convenor
口試委員
Advisory Committee
口試日期
Date of Exam
2000-11-07
繳交日期
Date of Submission
2001-08-07
關鍵字
Keywords
花環肉質軟珊瑚
Sarcophyton glaucun
統計
Statistics
本論文已被瀏覽 5671 次,被下載 1972
The thesis/dissertation has been browsed 5671 times, has been downloaded 1972 times.
中文摘要
摘要

海洋總是蘊育的一股神秘而未知的力量,隨著科技的日新月異,對於探討這人們多年來極欲瞭解的領域,有了相當程度上的助益,這不但使人類能對地球有更深一層的瞭解,對於海洋資源的開發與保護,能作更深入、更完善、更周詳的計劃。
海洋生物種類繁多,在提倡發展海洋資源之際,海洋生物便成為各大發展主題之一,其中天然物學家對於軟珊瑚其獨特,不具任何物理防禦機制的生存方式最感興趣。天然物學家認為,在海洋如此廣大的競爭生活方式下,若不具備任何防禦外侮的機制,必然走上滅絕之路,但是軟珊瑚仍然能生存至今,遂有其獨特的防禦方式。一般推論,其防禦方式乃屬化學防禦機制,經過多方的測試,亦得到了證明,許多軟珊瑚會分泌出一些對其它生物有毒殺活性的物質,來扼止其攻擊性的侵略,這奇特的防禦方式,更是引發許多生化學家的側目,對於人類探討抗菌、抗病毒、抗腫瘤的研究方向2,指引一道新的曙光
Abstract
Abstract

24ξ-methylcholestane-3β,5α,6β,25-tetrol-25-monoacetate (1), 24ξ-methyl
cholestane-3β,5α,6β,25-tetrol (2), 24ξ-methylcholestane-1β,3β,5α,6β,25-pentol
-25-monoacetate (3), (20S)-21-decanoxyl-24ξ-methylcholestane-3β,5α,6β,24 -tetrol (4), (20S)-21,24-diacetaoxy-24ξ-methylcholestane-3β,5α,6β-triol (5) were isolated from the soft coral Sarcopyton glaucum which were collected from the Green island. The structures of 1~5 were established by spectroscopic data , compound 1~3 have been reported previously, compound 4 and 5 are new metabolites.
The cytotoxicity of compounds 1~5 against A-549, HT-29 and P388 cancer
cell lines was studied. Only compound 1 showed significant cytotoxicity against
the growth of HT-29 cells line. Compound 2~5 were found to be inactive against
the above three cancer cells lines.



目次 Table of Contents
目錄
頁次
壹、緒論
一、前言 1
貳、Sarcophyton sp.軟珊瑚化學天然物之文獻回顧 3
參、生物採集與鑑種 10
一、採集 10
二、鑑種 10
肆、萃取分離流程與癌細胞活性測試 11
一、 萃取分離流程 11
二、 癌細胞活性測試 11
伍、器材與儀器 14
一、主要使用儀器 14
二、使用藥品 15
陸、Sarcophyton glaucum 軟珊瑚之化學成分分離與純化 16
化合物 (1) 之物理資料及數據 17
化合物 (2) 之物理資料及數據 18
化合物 (3) 之物理資料及數據 19
化合物 (4) 之物理資料及數據 20
化合物 (5) 之物理資料及數據 21
柒、Sarcophyton glaucum 軟珊瑚之化學成分之結構解析 22
化合物 (1) 結構解析 22
化合物 (2) 結構解析 28
化合物 (3) 結構解析 35
化合物 (4) 結構解析 42
化合物 (5) 結構解析 57
捌、生物活性 66
玖、結論 67
拾、參考文獻 68







參考文獻 References
拾、參考文獻
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10.Kobayashi, M.;Mitsuhashi, H."Glaucastrol, a Novel C27 sterol with a
unique side chain, from the Soft Coral Sarcophyton glaucum" Steroids ,1982, 40, 665.
11. Kobayashi, M.;Hayashi, T.;Hayashi, K;Tanabe, M.;Nakagawa,
T.;Mitsuhashi, H.”Polyhydroxysterols of the Soft Soral Sarcophyton
glaucum:Isolation and synthesis of 5α-cholestane-1β, 3β, 5, 6β-
tetrol” Chem. Pharm. Bull, 1983, 31,1848.
12.Kobayashi, M.; Ishizaka, T.; Mitsuhashi, H.”Isolation of 5α, 6-dihy droglaucasterol, a New Marine C27 sterol with a 24,26-cyclized Side chain, from the Soft Coral Sarcophyton glaucum”Chem.Pharm.Bull , 1983, 31, 1803.
13.Kobayashi, M.; Mitsuhashi, H.”Isolation of (24S)-24-methyl-5α-cho lestane-3β, 5, 6β, 25ξ, 26-pentol from the Soft Soral Sarcophyton glaucum” Chem. Pharm. Bull, 1983, 31, 4127.
14.Kobayashi, M.;Takako, I.;Emiko, N.”Marine Terpenes and Terpenoids. IX.1)Structure of Six New Cembranoids, Sarcophytols F, K, O, P, Q and S, from the Soft Coral Sarcophyton glaucum” Chem. Pharm. Bull, 1989, 37, 2053.
15. Kobayashi, M.;Katsunori, O.”Marine Terpenes and Terpenoids. VII.1)Minor Cembranoids Derivatives, Structurally Related to the Potent Anti-tumor-Promoter Sarcophytol A, from the Soft Coral Sarcophyton glaucum” Chem. Pharm. Bull, 1989, 37,631.
16.Kobayashi, M.; Kanda, F.; Rao, C. V. L.; Kumar, S. M. D.; Trimurlulu
, G.;Rao, C. B.”Polyhydroxysterols of the Soft Corals of the Andaman
and Nicobar Coasts.(1). Isolation of (24S)-24-methyl- cholest-5-ene-3β
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Pharm. Bull, 1990, 38, 1724.
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a New Cytotoxic Diterpene from Sarcophyton glaucum”Tetrahedron
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