本研究設計一個封閉的微渠道迴路，並以微型壓電幫浦做為流體驅動源產生剪力流，探討λDNA分子在此迴路中的動力特性。本實驗調配具三種黏滯度(40、60、80 cP)的五種緩衝溶液(1×TE、1×TAE、1×TBE、1×TPE、1×TBS)來拉伸λDNA分子，期望能利用高黏滯係數的緩衝溶液，配合封閉的迴路與渠道彎角處的流場變化，達到良好的拉伸效果。實驗觀測利用微質點影像測速儀(MicroParticle Image Velocimetry, μPIV)量測微渠道內的速度場，並配合螢光顯微鏡(Fluorescence Microscope, FM)觀察λDNA分子在渠道內受拉伸狀態，比較不同情況下，λDNA分子所受的力及其造成的形態變化和應力鬆弛時間，進而計算應變率，統計不同參數下的拉伸率，並與一般常用的受力模型做比較。實驗結果發現，在高Wi的溶液中DNA分子拉伸的長度分佈較為平均，所得到的拉伸率也較高，而彎曲渠道所造成的流場特性也有助於DNA分子的拉伸。

In this study, closed-loop microchannel with 180° bend was designed to investigated the dynamics of DNA molecules in the microchannel, and piezoelectric diaphragm micropump was used to generated shear flow. The deformation and conformation of labeled λDNA was directly observed by fluorescence microscope, and μPIV was built to measure the velocity distribution in microchannel. This study also analyzed statistically stretch length distribution and relaxation time of DNA molecules in order to clarify the effect of Weissenberg number on the stretching of DNA molecules. The shear rate and force exerted on DNA molecules was calculated and compared with conventional stretch model. It was found that higher Wi could make the stretch length distribution more averaged, and the curved microchannel increased the shear rate.

符號說明 vi
中文摘要 ix
Abstract x
第一章 序論 1
1-1 前言 1
1-2 DNA分子結構與性質 1
1-3 微機電系統與生醫晶片 2
1-4 背景與目的 4
1-5 文獻回顧 7
第二章 實驗系統與設備 18
2-1 微質點影像測速儀系統 18
2-2 螢光顯微鏡系統 19
2-3 製程設備 20
2-4 其他實驗週邊設備 21
第三章 實驗方法及步驟 29
3-1 微流道設計與製程 29
3-2 工作流體配製 32
3-3 μPIV量測系統建立及原理 33
3-4 螢光顯微鏡量測系統建立及原理 34
3-5 實驗量測參數 34
第四章 理論分析 43
4-1 DNA流變學理論 43
4-2 流場無因次參數分析 45
第五章 誤差分析 49
第六章 結果與討論 53
6-1 μPIV流場量測 53
6-2 DNA分子螢光觀測 57
6-3 DNA分子受力分析 63
6-4 DNA分子拉伸模型 67
第七章 結論與建議 92
7-1 結論 92
7-2 建議與改進 93
參考文獻 95
附錄A 105

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