Title page for etd-0810113-130547


[Back to Results | New Search]

URN etd-0810113-130547
Author Chun-pao Tai
Author's Email Address dai192@ptpolice.gov.tw
Statistics This thesis had been viewed 5095 times. Download 381 times.
Department Biological Sciences
Year 2013
Semester 1
Degree Master
Type of Document
Language zh-TW.Big5 Chinese
Title Detection of microRNA expression in the blood of CCl4-induced liver fibrotic mice
Date of Defense 2013-08-30
Page Count 75
Keyword
  • liver fibrosis
  • miRNA
  • Drosha
  • gene-specific reverse transcriptase-PCR
  • Dicer
  • Abstract Abstract
       MiRNAs are an emerging class of highly conserved non-coding small RNAs that regulate gene expression at the post-transcriptional level. Most miRNAs are generated by RNA polymeraseⅡ as long primary transcripts (pri-miRNAs) that form a stem-loop structure. In the nucleus, pri-miRNAs are processed into 70-100 nucleotide long hairpin pre-miRNAs by the RNAse Ⅲ Drosha. These pre-miRNAs are then exported into the cytoplasm by exportin-5. They are then further processed by another RNAse Ⅲ, Dicer. The resultant approximately 22-nucleotide RNA duplexes contain the mature miRNA and the passenger miRNA strand. MiRNAs dysregulation frequently occur in liver disease. Treatment with carbon tetrachloride (CCl4) is a most common model for liver fibrosis study in rodents. Analysis of whole blood miRNAs in CCl4-induced hepatic fibrosis in mice model has not been reported, but could potentially lead to novel assays for early detection and monitoring of hepatic fibrosis. To determine which miRNAs associated with hepatic fibrosis in the peripheral blood, we used gene-specific reverse transcriptase-PCR to screen pre-miRNA profiles in whole blood and liver obtained from CCl4-treated, oil-treated and PBS-treated mice. Blood were collected from mice at different stages of hepatic fibrogenesis. We have identified specific miRNA expression profiles that correlated with hepatic fibrosis and its stage. Our results suggest that specific changes in blood pre-miRNA may be potential biomarkers during progression of hepatic fibrosis.
    Advisory Committee
  • Chao-neng Tseng - chair
  • Chien-Chih Chiu - co-chair
  • Chung-Lung Cho - advisor
  • Files
  • etd-0810113-130547.pdf
  • Indicate in-campus at 2 year and off-campus access at 2 year.
    Date of Submission 2013-09-10

    [Back to Results | New Search]


    Browse | Search All Available ETDs

    If you have more questions or technical problems, please contact eThesys