近來利用核磁共振(NMR)方法鑑定生物分子被廣泛的應用。許多二維NMR實驗更是分子內原子之間相互關係的有力證據。而一般來說影響生物分子的因素包括了濃度、酸鹼環境、溫度。將核苷酸分子AMP、ADP重水溶液分別配製成pH值分別為1、4、7、10、13的酸鹼環境，以及將AMP、ADP重水溶液(pH = 7)置於150C、250C、350C、450C、550C之下做分子內各核種(1H、13C、23Na、31P)的一維、二維NMR測量。視酸鹼、溫度對AMP、ADP的影響為何 。

Recently years NMR technique plays an important role in investigation of structures of biochemistry molecules. Especially two-dimension NMR experiments are powerful evidences of the relation of intramolecule of biochemistry. Further more we design a series of experiments about studies of chemical shift and dynamics under different pH values and at different temperature.

目錄
圖目錄
表目錄
壹、 緒論
一、 NMR的基本介紹
1. NMR的基本原理
2. 二維核磁共振光譜(2D NMR Spectrum)
二、 NMR技術的應用
1. 磷的偵測
2. 生物分子與金屬的作用
三、 研究動機
貳、 實驗方法
一、 儀器設備
二、 實驗藥品
三、 樣品配製
四、實驗參數
參、 結果與討論
一、 一維實驗
二、 二維實驗
肆、 結論
伍、 參考資料

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