子宮頸癌是全世界女性腫瘤中造成許多患者死亡的癌症之一。雖然子宮頸抹片檢查已經發展成為有效的偵測子宮頸癌癌症初期的篩選方式。但是我們對於病毒與造成人類癌症之發展機轉仍然未明。活化細胞訊息傳遞路徑JAK/STAT中的signal transducer and activator of transcription 3 (STAT3)已經被並證實與許多癌症形成有關。然而在STAT3之酪氨酸705位置磷酸化(p-tyr705-STAT3)和STAT3絲氨酸727位置磷酸化(p-ser727-STAT3)在子宮頸癌的形成機轉中的功能及影響仍未明朗。我們研究主要的目的是為了要了解活化STAT3之酪氨酸705位置磷酸化與絲氨酸727位置磷酸化分別在子宮頸癌的形成所扮演的角色。研究結果顯示臨床上當病患有較高p-tyr705-STAT3、p-ser727-STAT3與Ki67(細胞增生標記)的表現量時，這些病患預後較差。

Cervical cancer is one of the most important female deaths caused by cancer in the world. Although the Pap smear has become an effective screening method for early detection of cervical cancer.The cause of human cancer by virus and mechanisms of cancer development are still unclear.Activation of signal transducer and activator of transcription 3 (STAT3) in JAK/STAT signaling pathway has been observed during the development of cancer formation.
However,the roles play by the phosphorylated tyrosine residue 705 (p-tyr705-STAT3) and phosphorylated serine residue 727 (p-ser727-STAT3) of STAT3 in cervical carcinogenesis of human clinical specimens are not clear.
Our research aimed to understand the role of phosphorylation of signal transducer and activator of transcription 3 (STAT3) at the tyrosine residue 705 and the serine residue 727 respectively.The results indicated that patients with higher expression of both activated residues,p-tyr705 and p-ser727,and Ki67,a cell proliferation marker,showed poor prognosis significantly.

論文審定書 i
中文摘要 ii
英文摘要 iii
目錄 iv
圖次 vi
表次 vii
第一章 背景介紹 1
1.1 子宮頸癌 1
1.2 STATs 蛋白質 2
1.3 STATs 蛋白質簡介 3
1.4 STATs蛋白質的結構 4
1.5 STATs蛋白質的活化機制 4
1.6 STATs蛋白質與腫瘤形成的相關性 5
1.7 STAT3蛋白質 6
1.8 STAT3蛋白質與腫瘤發生之關係 6
1.9 Ki67蛋白質 7
第二章 實驗目的 8
第三章 實驗材料和方法 9
3.1檢體收集與保存 9
3.2 化學免疫組織染色法 9
3.2.1石蠟包埋組織切片 9
3.2.2 化學免疫組織染色(Immunohistochemistry,IHC)試劑 9
3.2.3化學免疫組織染色法步驟 10
3.2.4化學免疫組織染色結果評估 11
3.3統計分析 11
第四章 實驗結果 12
第五章 討論 14
參考文獻 15
圖表 22
附錄圖表 34

中華民國行政院衛生福利部國民健康署癌症防治網於2013年5月6日公告癌症統計資料(Http://www.hpa.gov.tw)
行政院衛生署國民健康局.中華民國100 年子宮頸癌篩檢登記報告資料(Http://www.hpa.gov.tw)
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