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博碩士論文 etd-0813117-102236 詳細資訊
Title page for etd-0813117-102236
論文名稱
Title
探討BCL6基因在膀胱尿路上皮癌細胞株中影響Cisplatin敏感性反應變化及其分子機制
Studies the effect of BCL6 gene on the sensitivity of Cisplatin in bladder urothelial carcinoma cell lines
系所名稱
Department
畢業學年期
Year, semester
語文別
Language
學位類別
Degree
頁數
Number of pages
57
研究生
Author
指導教授
Advisor
召集委員
Convenor
口試委員
Advisory Committee
口試日期
Date of Exam
2017-08-28
繳交日期
Date of Submission
2017-09-13
關鍵字
Keywords
抗藥性、細胞凋亡、CDC42、B-cell lymphoma 6 protein (BCL6)、膀胱癌、TP53、順鉑
Bladder cancer, TP53, B-cell lymphoma 6 protein (BCL6), Apoptosis, Drug resistance, Cisplatin, CDC42
統計
Statistics
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中文摘要
Cisplatin 是化療的重要用藥,對於膀胱癌,小細胞肺癌及卵巢癌均有良好的治療效果。不過有部分泌尿上皮癌病患使用Cisplatin治療一段時間後會產生耐藥性,進而影響治療效果。為此,了解泌尿上皮癌細胞對於Cisplatin耐藥性的作用機轉是相當重要的。而B-cell lymphoma 6 protein (BCL6),是一種transcription repressor,會與transcription factor交互作用,而抑制細胞核的轉錄作用,並會抑制DNA repair及TP53的活性。本篇研究中使用人類膀胱癌細胞株 (BFTC905、T24),並以遞增Cisplatin濃度的方式,篩選出具有Cisplatin耐藥性的T24 (T24R1、T24R2)。我們想藉由比較上述幾個細胞株,在BCL6 gene 及其可能參與的相關路徑下游基因之mRNA及Protein level上的差異,進而探討造成耐藥性的相關作用機制。
Abstract
Cisplatin is one of the most effective anticancer drugs used for treatment of tumors, including bladder, small cell lung and ovarian cancers. During the cisplatin treatment of urothelial cancer, some patients will become to be cisplatin resistance, and then it will influence the outcome of treatment. Therefore, it is an important topic how to characterize the cisplatin-resistant mechanism in human urinary bladder urothelial carcinoma (UBUC) cells. B-cell lymphoma 6 protein is transcription repressor and interaction with transcription factor, regulates gene expression. BCL6 is proto-oncogene and it inhibit DNA repair pathways and TP53 protein. According to the report, we find that the BCL6 overexpression in many cancer cell. Our use the array comparative genomic hybridization, explore the BCL6 overexpression in bladder cancer cell. Thus, we suspect that BCL6 have the important role in bladder cancer. To evaluate the BCL6 gene in urinary bladder urothelial carcinoma-derived cell lines would sensitize their responses to cisplatin and study the underlying molecular mechanisms.
目次 Table of Contents
論文審定書………………………………………………………………..i
致謝 ………………………………………………………………………ii
中文摘要 …………………………………………………………………iii
英文摘要 …………………………………………………………………iv
英文縮寫表 ………………………………………………...……….……v
目錄 ………………………………………………………………………vi
圖次 …………………………………………………………………...…vii
表次 ……………………………………………………………….…….viii
壹、 緒論 (Introduction) …………………………………………………1
貳、 實驗材料與方法 (Materials and methods)……………….……..10
參、 結果 (Results)……………………………………………………...28
肆、 討論 (Discussion) …………………………………………………41
伍、 參考文獻 (References) …………………………………...………42
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