本研究探討飼料油脂組成對海鱺稚魚成長、體組織脂肪細胞密度與直徑大小、肝臟脂肪代謝相關酵素活性及組織脂肪酸組成的影響。實驗飼料以魚粉及大豆蛋白作為主要蛋白質來源，油脂含量為15%，其中6%為魚油，提供足量的必需脂肪酸，另外9%分別為魚油﹙HUFA, n-3﹚﹙FO﹚、紫蘇油﹙18:3 n-3﹚﹙PE﹚、紅花油﹙18:2 n-6﹚﹙SA﹚、橄欖油﹙18:1 n-9﹚﹙OL﹚、棕櫚油﹙16:0﹚﹙PA﹚，飼養初重為80克海鱺十週。餵食含有較高飽和脂肪酸的PA組飼料之海鱺有最大末重，SA組最小。SA組全魚體粗脂質含量最高，且肝臟、腹肉及背肉粗脂肪含量亦是五組中最高的。OL組全魚體粗蛋白含量最低，PA組全魚體灰份含量顯著﹙p<0.05﹚高於其他組別。FO組全魚體粗蛋白及水分含量最高，粗脂肪及灰份最低。各組肝臟、腹肉、及背肉脂肪細胞密度並沒有隨著時間而增加的趨勢，但腹部脂肪團脂肪細胞密度有隨著時間而增加的情形。當餵食全魚油飼料時海鱺肝臟、腹肉及背肉脂肪細胞密度最低，而肝臟蘋果酸比活性隨著時間先增後減，脂肪酸合成&#37238;比活性隨著時間降低。食用紫蘇油飼料時海鱺肝臟、腹肉及背肉脂肪細胞密度較低，此時且背肉脂肪細胞直徑較大，而肝臟脂肪酸合成&#37238;有隨著時間而降低的情形。餵食紅花油或棕櫚油飼料的海鱺有最大脂肪細胞密度，且背肉脂肪細胞直徑較大。投餵橄欖油飼料的海鱺腹部脂肪團脂肪密度較高，背部脂肪細胞粒徑較小。以植物油取代部分魚油會改變魚體組織脂肪酸組成，魚體組織中HUFA含量減少，EPA/DHA提高，n-3/n-6降低，而MUFA、PUFA及SAF比例明顯增加。綜合以上結果，不同的植物油脂會影響海鱺幼魚脂肪細胞在魚體各部位之大小、密度及分佈、肝臟脂肪酸合成之路徑及體脂肪酸之組成，其中餵食紅花油及棕櫚油飼料會顯著增加脂肪細胞密度與大小，這些結果可用於調節幼魚脂肪細胞含量，將來做為成魚肥育之良好?眲W。

This research studied the dietary effect of supplemental plant oils on growth, adiposity and lipid metabolism-related enzyme activity of juvenile cobia. The isonitrogenous and isoenergetic basal diet contained 15% crude lipid, 6% fish oil and 9% supplemental oils. The supplemental oils were varied among 5 dietary treatments, including fish oil (HUFA, n-3)(FO), perilla oil (18:3 n-3)(PE), safflower oil (18:2 n-6)(SA), olive oil (18:1 n-9)(OL), and palm oil (16:0)(PA). Results of the 10-wk feeding trial show that fish fed diet containing palm oil had the highest final weight, and was significant higher than fish fed SA diet. SA group had highest crude lipid concentration. OL group had the least crude protein concentration. PA group had the highest ash concentration. FO group had the highest crude protein and moisture content, the least crude lipid and ash content. Adipocyte density in various, tissues did not vary with time, except the ventral fat depot. Tissue adipocyte density of FO group was the least. Adipocyte density of PE group was higher than FO group, and its mean adipcoyte diameter in dorsal muscle was great than the other groups. Fatty acid synthase (FAS), as measured by specific activity, decreased with times in the PE group. Adipocyte density of SA group the highest of all groups, and its mean adipocyte diameter in dorsal muscle was also the greatest. Adipocyte density in the ventral fat depot of OL group was the highest, and its mean diameter in dorsal muscle was the smallest. Adipocyte density of PA group was only slightly lower than SA group. The tissue acid composition of the cobia was influenced by the supplemental plant oils. Tissue HUFA concentration and n-3/n-6 ratio was decreased, MUFA, PUFA and SAF composition was increased when the plant oils were supplemented. The results show that the supplementation of the plant oils could affect the density , size and tissue distribution of adipocytes, fatty acid synthesis pathway in the liver and tissue fatty acid composition. Feeding the cobia diet containing supplemental safflower or palm oil significantly increased density and cell size of adipocytes in the tissue of the cobia.

中文摘要……………………………………………………………………………I
英文摘要……………………………………………………………………………III
目錄…………………………………………………………………………………V
表目錄………………………………………………………………………………VI
圖目錄………………………………………………………………………………X
附錄目錄……………………………………………………………………………XII
文獻回顧……………………………………………………………………………1
前言…………………………………………………………………………………16
材料方法……………………………………………………………………………19
結果…………………………………………………………………………………41
討論…………………………………………………………………………………98
結論…………………………………………………………………………………106
參考文獻……………………………………………………………………………107
附錄…………………………………………………………………………………127

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