本研究從台灣產軟珊瑚 Klyxum molle的有機層萃取物中，尋找出具有生物活性的海洋天然化合物。軟珊瑚 Klyxum molle的乙酸乙酯粗萃物經由反覆的管柱層析，分離純化出九個新的雙萜化合物 klymollins V－Z (1－5) 和 klyxumollins A－D (6－9) 及四個已知的eunicellin類雙萜化合物 (10－13)。新化合物的化學結構解析是由各種光譜 (IR、MS、1D及2D NMR) 的分析，並與文獻上相似化合物的光譜資料比較而確定。
本論文亦將所獲得的化合物1－13進行人類血癌細胞株 (CCRF-CEM、K562 和 Molt 4)、人類乳癌細胞株 (T47D) 與人類結腸癌細胞株 (DLD-1) 的細胞毒殺活性測試與抗發炎活性測試。化合物2對3株癌細胞 (CCRF-CEM、Molt 4和T47D) 有微弱的細胞毒殺活性，化合物4和9只對CCRF-CEM有微弱的細胞毒殺活性，而化合物11對CCRF-CEM 有較好的活性表現 (ED50 = 4.2 μg/mL)。在抗發炎活性測試方面，化合物3在25 μM的濃度下對由LPS誘發老鼠巨噬細胞所產生的促發炎細胞激素IL-6有微弱的抑制效果。

In order to discover marine bioactive compounds, we investigated the chemical constituents from the organic extracts of soft coral Klyxum molle. Repeated column chromatography on the ethyl acetate extract of soft coral Klyxum molle, resulted in the isolation of nine new eunicellin-type diterpenoids, klymollins V－Z (1－5) and klyxumollins A－D (6－9), and four known compounds (10－13). The structures of new compounds were determined on the basis of extensive spectroscopic data (IR, MS, 1D and 2D NMR) and by comparison of the spectral data with those of the related known compounds.
The cytotoxicity of compounds 1－13 was evaluated against five tumor cell lines including CCRF-CEM (human T cell lymphoblast-like cell line), K562 (human erythromyeloblastoid leukemia cell line), Molt 4 (human acute lymphoblastic leukemia cell line), T47D (human ductal breast epithelial tumor cell line) and DLD-1 (human colorectal adenocarcinoma cell line). Compound 2 exhibited weak cytotoxicity toward three cancer cells (CCRF-CEM, Molt 4 and T47D) and compounds 4 and 9 showed weak cytotoxicity against CCRF-CEM cancer cells. Compound 11 was found to exhibit stronger cytotoxicity toward CCRF-CEM cancer cells (ED50 values of 4.2 μg/mL). On the other hand, compound 3 showed weak activity against the accumulation of the pre-inflammatory cytokine IL-6 in LPS-induced macrophages, at a concentration of 25 μM.

目錄
論文審定書 i
誌謝 ii
中文摘要 iii
英文摘要 iv
化合物1-13化學結構 v
圖次 ix
表次 xiv
第一章、緒論 1
第一節、前言及研究動機 1
第二節、文獻回顧 3
第二章、生物材料與研究方法 41
第一節、Klyxum molle樣品採集及實驗流程 41
第二節、Klyxum molle 分離流程 43
第三節、實驗設備儀器及材料 45
第三章、化合物之結構證明 48
第一節、軟珊瑚Klyxum molle 所分離出之化合物的結構證明 48
(一)、Klymollin V (1) 結構之解析 48
(二)、Klymollin W (2) 結構之解析 58
(三)、Klymollin X (3) 結構之解析 68
(四)、Klymollin Y (4) 結構之解析 78
(五)、Klymollin Z (5) 結構之解析 88
(六)、Klyxumollin A (6) 結構之解析 98
(七)、Klyxumollin B (7) 結構之解析 109
(八)、Klyxumollin C (8) 結構之解析 120
(九)、Klyxumollin D (9) 結構之解析 130
(十)、Sclerophytin A (10) 結構之解析 141
(十一)、Sclerophytin B (11) 結構之解析 145
(十二)、Sclerophytin F methyl ether (12) 結構之解析 149
(十三)、(1R*, 2R*, 3R*, 6S*, 9R*, 10R*, 14R*)-3-Acetoxycladiell-7(16), 11(17)-dien-6-ol (13) 結構之解析 154
第二節、化合物物理及光譜資料 157
第四章、生物活性試驗 160
第一節、生物活性試驗方法 160
(一)、細胞毒殺活性試驗方法 160
(二)、抗發炎活性試驗方法 162
第二節、生物活性試驗結果 164
(一)、細胞毒殺活性試驗結果 164
(二)、抗發炎活性試驗結果 165
第五章、結論 167
第六章、參考文獻 170

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