針對台灣東北角鶯歌石附近海域所採集的軟珊瑚Sinularia sp.所具有的化學成分研究中，共分離出十二個天然化合物，分別為sinularioperoxides A-D（1-4）、sinulariolins A-D（5-8）、1a,3b-Di-hydroxy-24-methylencholesta-5,9-diene（9）、（3S）3,7-Dimethyl-nona-6,8-dienoic acid（10）、（2¢S）-Ethyl（5¢E）-5-(2¢,6¢-dimethylocta-5¢,7¢-dienyl)furan-3-carboxylate(11）、3'R,4'-((2R,4S)-2-Hydroxy-4-acetoxy-2,6,6-trimethyl-cyclohexylidene)but-3'-en-2'-one（12），其中十一個為新發現的天然化合物（1-11），而化合物12曾在藻類和植物中被發現。化合物（1-12）之化學結構是以各種光譜（1D NMR、2D NMR、IR、MS、UV）資料確立。

The chemical constituents of EtOH extracts of the soft coral Sinularia sp. have led to the isolation of nature compounds（1-12）,sinularioperoxides A-D（1-4）, sinulariolins A-D（5-8）, 1a,3b-Dihydroxy-24-methylencholesta-5,9-diene（9）,（3S）-3,7-Dimethyl-nona-
6,8-dienoicacid（10）, （2¢S）-Ethyl（5¢E）-5-(2¢,6¢-dimethylocta-5¢,7¢-dienyl)furan-3-carboxylate（11）, and one know metabolite, 3'R,4'-((2R,
4S)-2-hydroxy-4-acetoxy-2,6,6-trimethyl-cyclohexylidene)but-3'-en-2'-one（12）, respectively. Compounds 1-11 are new and 12 is a know metabolite, which had been isolated from the brown alga and the plant. The structures of 1-12 were elucidated by the interpretation of spectroscopic data（UV, MS, IR, 1D and 2D NMR）.

中文摘要 1
英文摘要 2
壹、緒論
一、前言 5
二、研究動機 6
三、過去相關研究概要

(一)、具有cyclic peroxide的sesquiterpene化合物文獻回顧 7
(二)、Valerenane type skeleton化合物文獻回顧 13
(三)、Eudesmane sesquiterpenes化合物文獻回顧 19
(四)、Sterols type skeleton具雙鍵D5,6、D9,11的化合物
文獻回顧 22
(五)、Furanosesquiterpenes 文獻回顧 24
貳、實驗程序與方法

一、生物樣品採集時間、地點與分類地位 26
二、萃取與分離純化步驟 27
三、實驗器材與溶劑 28

參、細胞毒殺活性測試

一、生物活性測試 30
二、實驗採用之癌細胞株 32

肆、實驗結果與討論

一、軟珊瑚Sinularia sp.化合物之結構解析

(一)、Sinularioperoxide A (1)之結構解析 33
(二)、Sinularioperoxide B (2)之結構解析 58
(三)、Sinularioperoxide C (3)之結構解析 72
(四)、Sinularioperoxide D (4)之結構解析 88
(五)、Sinulariolin A (5)之結構解析 102
(六)、Sinulariolin B (6)之結構解析 115
(七)、Sinulariolin C (7)之結構解析 131
(八)、Sinulariolin D (8)之結構解析 148
(九)、1a,3b-Dihydroxy-24-methylencholesta-5,9-diene (9) 163
(十)、（3S）-3,7-Dimethyl-nona-6,8-dienoic acid (10) 178
(十一)、（2¢S）-Ethyl（5¢E）-5-(2¢,6¢-dimethylocta-5¢,7¢-
dienyl)furan-3-carboxylate (11) 191
(十二)、3'R,4'-((2R,4S)-2-Hydroxy-4-acetoxy-2,6,6-tri-
methyl-cyclohexylidene)but-3'-en-2'-one (12) 205


伍、結論 217

陸、參考文獻 219

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