Title page for etd-0819109-130742


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URN etd-0819109-130742
Author Hui-mei Chan
Author's Email Address m955020032@student.nsysu.edu.tw
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Department Marine Biotechnology and Resources
Year 2008
Semester 2
Degree Master
Type of Document
Language zh-TW.Big5 Chinese
Title Self-splicing of Group I Intron of the Mitochondrial Genome of the Sponge, Cinachyrella australiensis
Date of Defense 2009-06-05
Page Count 146
Keyword
  • group I intron
  • cyclization
  • self-splicing
  • C. australiensis
  • homing endonuclease
  • Abstract Intragenic regions (introns) are found in all classes of organism. Transcription of such genes must undergo a splicing reaction to produce the mature, functional form of RNAs. Introns can be divided into four categories by their splicing mechanisms, namely Group I, Group II, spliceosomal, and nuclear tRNA introns. The former two are self-splicing introns. Group I introns are ubiquitous, however, most metazoan mitochondrial genomes lack introns. A novel group I intron in the mitochondrial cytochrome oxidase I gene (cox1) of Cinachyrella auctraliensis, which belongs to the IB2 subgroup, encodes a putative homing endonuclease with two amino acid motifs of the LAGLIDADG family. The homing endonuclease may perform intron translocation. Splicing in the cox1 of the sponge was demonstrated by comparing the length of DNA and RNA sequences. The intron was spliced in vivo or in vitro as revealed by RT-PCR and sequencing. Group I introns are classified as ribozymes. The pre-mRNAs fold into specific configurations that facilitate attacks of free guanosine followed by two consecutive trans-esterification steps to remove the introns. The excised cox1 intron was found to form a circle with the 5’-end linked to the 3’-end. Two other forms of lariats were also found with the 5’-end linked to the inside sequence of the intron. Mutagenesis of a key nucleotide, which participates base pairing of RNA secondary structure, in P7 region decreased the splicing activity of the intron.
    Advisory Committee
  • Zhi-Hong Wen - chair
  • Chan-Shing Lin - co-chair
  • Chi-Hsin Hsu - advisor
  • Files
  • etd-0819109-130742.pdf
  • indicate not accessible
    Date of Submission 2009-08-19

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