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博碩士論文 etd-0822100-153322 詳細資訊
Title page for etd-0822100-153322
論文名稱
Title
蚵岩螺雄化程度與MFO酵素系統活性相關之研究
The study of the enzymes of the mixed function oxidase(MFO)system and its relation to the imposex of Thais spp.
系所名稱
Department
畢業學年期
Year, semester
語文別
Language
學位類別
Degree
頁數
Number of pages
60
研究生
Author
指導教授
Advisor
召集委員
Convenor
口試委員
Advisory Committee
口試日期
Date of Exam
2000-07-14
繳交日期
Date of Submission
2000-08-22
關鍵字
Keywords
單氧酵素、雄性化
mixed function oxidase (MFO) system, imposex
統計
Statistics
本論文已被瀏覽 5663 次,被下載 3331
The thesis/dissertation has been browsed 5663 times, has been downloaded 3331 times.
中文摘要
環境中有機錫化合物引發螺類的雄化現象,在世界各地均有報導,但雄化與貝類解毒能力之關係仍屬未知,因此,本研究即針對此一問題進行探討。本研究以本省西海岸潮間帶常見之蚵岩螺為材料,在香山、台中港、布袋、七股及東港五個地點採回常見的三種蚵岩螺:Thais clavigera、Thais rufotincta及Thais sp.,觀察型態上雄化的現象並測量體內的單氧酵素活性。雄化程度以雄化百分比、相對陰莖指數(RPS)及輸精管指數(VDS)來表示,另以測定cytochrome P450 、NADPH-cytochrome c reductase、EROD(7-ethoxyresorufin-O-deethylation)及cytochrome P420來代表單氧酵素系統活性。結果發現三種蚵岩螺的雄化百分比、相對陰莖指數(RPS)及輸精管指數(VDS)分別為71-100﹪、8.8-62.5﹪及0.7-2.5;其中以T. clavigera的雄化程度最高、T. sp.次之,而以T. rufotincta最低。蚵岩螺體內的cytochrome P450及cytochrome P420的含量分別為0.043-0.097 nmol/mg protein及0.129-0.296 nmol/mg protein;此外,蚵岩螺體內的NADPH- cytochrome c reductase的活性為1.52-6.73 nmol/min/mg protein,而EROD的活性則測不到。蚵岩螺雄性化的程度與cytochrome P450及cytochrome P420的含量呈現負相關的趨勢,雄性化的程度與NADPH-cytochrome c reductase的活性呈現正相關之趨勢。雄性化的程度與cytochrome P450的含量呈現負相關趨勢之結果,支持雄化可能是由於cytochrome P450活性受到抑制導致螺體內的荷爾蒙代謝失調引起之假說。
Abstract

Induction imposex of gastropod snails by organotins has been reported worldwide. However, the relationship between imposex and detoxification capability is not well studied in molluscs. In the present study, the relationship between the mixed function oxidase (MFO) system and the degree of imposex in drills Thais spp. was investigated. Thais clavigera, T. rufotincta and T. sp. were collected from the west coast of Taiwan. The degree of imposex was determined by percent imposex, relative penis size (RPS) and vas defenes sequence (VDS). Enzymes of cytochrome P450, NADPH-cytochrome c reductase, EROD(7-ethoxyresorufin-O-deethylation) and cytochrome P420 (an inactive form of cytochrome P450) in the MFO system were examined. In the three Thais species, the degrees of imposex expressed as percent imposex, RPS and VDS were 71-100%, 8.8-62.5% and 0.7-2.5, respectively. Contents of cytochrome P450 and cytochrome P420 were 0.043-0.097 nmol/mg protein and 0.129-0.296 nmol/mg protein, respectively. The activity of NADPH-cytochrome c reductase was in the range of 1.52-6.73 nmol/min/mg protein. The activity of EROD was not detected. Negative trends between the degree of imposex and the contents of cytochrome P450 and cytochrome P420 were found in all the Thais species. And, a positive trend betweed the degree of imposex and the activity of NADPH-cytochrome c reductase was also observed. Based on the results, it is suggested that the induction of imposex was possibly due to the imbalance of steroid hormone metabolism caused by the inhibition of cytochrome P450 activity.

目次 Table of Contents

目 錄

章次 頁次
中文摘要…………………………..Ⅲ
英文摘要…………………………..Ⅳ
謝辭………………………………..Ⅴ
目錄………………………………..Ⅵ
表目錄………………………….….Ⅶ
圖目錄……………………………..Ⅷ
附錄………………………………..Ⅸ
一、 緒論……………………..1
二、 材料與方法………………7
三、 結果…………………….12
四、 討論…………………….18
參考文獻……………………………26
個人履歷……………………………52


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