Title page for etd-0822112-152637


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URN etd-0822112-152637
Author Che-Wei Hu
Author's Email Address m985010012@student.nsysu.edu.tw
Statistics This thesis had been viewed 5099 times. Download 178 times.
Department Marine Biology
Year 2011
Semester 2
Degree Master
Type of Document
Language English
Title Isolation and Characterization of a new thermotolerant pigment- producing microalga: Salt stress enhances pigment and oil biosynthesis in Coelastrella sp.F50
Date of Defense 2012-07-30
Page Count 38
Keyword
  • natural pigments
  • salt stress
  • Coelastrella
  • thermotolerance
  • microalgae
  • Abstract A new species of reddish-orange pigment-producing microalga was isolated from a shallow pond in tropical Taiwan. Morphological and molecular evidence including meridional ribs on the cell wall, pigment production, and 18S rDNA sequence analysis suggest that this microalga is a species in the genus Coelastrella. Salt stress accelerated biosynthesis of the reddish-orange pigments, and large quantity of oil accumulated as the cells stressed under nutrient deficiency. This microalga could
    sustain 45 °C for more than 8 hours indicated by the stability of its chlorophylls, which is a necessary trait for large scale outdoor cultivation using photobioreactors in tropical areas. The reddish-orange pigments could be separated into many fractions by HPLC, and signals from carotenoids were detected in a few fractions using NMR, suggesting these pigments may function as antioxidants among other roles.
    Advisory Committee
  • Chih-Sheng Lin - chair
  • Lee-Feng Chien - co-chair
  • Ching-Nen Nathan Chen - advisor
  • Files
  • etd-0822112-152637.pdf
  • Indicate in-campus at 3 year and off-campus access at 3 year.
    Date of Submission 2012-08-22

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