Title page for etd-0828111-002130


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URN etd-0828111-002130
Author Yu-Shan Chen
Author's Email Address No Public.
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Department Biological Sciences
Year 2010
Semester 2
Degree Master
Type of Document
Language zh-TW.Big5 Chinese
Title The Angiogenic Effects of β-endorphin in Endothelial Cells
Date of Defense 2011-07-07
Page Count 52
Keyword
  • angiogenesis
  • morphine
  • opioid receptor
  • endothelial cells
  • β-EP
  • Abstract Angiogenesis is a fundamental process in reproduction and wound healing. Angiogenesis is also indispensable for solid tumor growth and metastasis, and also associated with angiogenic diseases. Beta-endorphin (β-EP), derived from its precursor pro-opiomelancortin (POMC), is well known for its role in nociception and immune regulation. However, the function of morphine and β-EP during angiogenesis remains characterization. One previous study indicated that morphine inhibited the proliferation and hypoxia-induced vascular endothelial growth factor (VEGF) release of endothelial cells. Contrastingly, another report found that morphine via Ras/PI3k/MAPK/ERK signaling promotes the survival and angiogenesis in endothelial cells. Besides, endogenous opioid peptides stimulated angiogenesis in chicken allantoic membrane assay through opioid receptors. Thus, the function and mechanism of β-EP and opioid receptors in angiogenesis are controversial. This study evaluated the culture effects of β-EP and morphine on angiogenesis . It was found that β-EP stimulated the proliferation, migration, and tube formation of endothelial cells in a dose-dependent manner. Morphine at a high dose inhibited the proliferation, migration, and tube formation of endothelial cells. In the ex vivo rat aortic ring assay, β-EP enhanced, whereas morphine perturbed, the microvessel sprouting. We also confirmed the expression of MOR,DOR,KOR opioid receptor in endothelial cells. Application of naloxone, a selective opioid antagonist, and neutralizing antibodies of MOR abolished the angiogenic effect of β-EP and morphine. Thus β-EP and morphine exert the pro- and anti-angiogenic effect via MOR, respectively .Besides, β-EP can be regarded as a novel angiogenic factor. 
    Advisory Committee
  • Ching-Mei Hsu - chair
  • Feng-sheng Wang - co-chair
  • Ming-Hong Tai - advisor
  • Files
  • etd-0828111-002130.pdf
  • Indicate in-campus at 0 year and off-campus access at 3 year.
    Date of Submission 2011-08-28

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