七個新eunicellin-based 雙萜化合物 krempfielins K－Q (1－7)，伴隨著六個已知化合物，litophynin I (8)，krempfielin A (9)，krempfielin D (10)，sclerophytin F (11)，litophynol B (12) 和 litophynol I monoacetate (13)，是從軟珊瑚 Cladiella krempfi中分離出來，這株軟珊瑚是從澎湖採集而來的。這些化合物的結構是由光譜數據分析 (IR, MS, 1H NMR, 13C NMR, HSQC, HMBC, COSY, and NOESY) 並比對文獻上已知化合物之光譜數據而定的。化合物1的結構較少見，是由於從C-2與 C-6 經氧原子連接組成 tetrahydropyran ring。化合物7是目前第一次發現為 eunicellin-based 的 dimer。
化合物 1–6 和 8–13 以 10 μM 的濃度進行抗發炎活性測試。結果顯示，化合物 4 可以抑制超過 70% 在 FMLP/CB-induced human neutrophils 的 elastase 釋放表現。

Seven new eunicellin-based diterpenoids, krempfielins K－Q (1－7), along with six known compounds, litophynin I (8), krempfielin A (9), krempfielin D (10), sclerophytin F (11), litophynol B (12), and litophynol I monoacetate (13), have been isolated from the soft coral Cladiella krempfi which was collected from Penghu Island of Taiwan. Their structures were determined through extensive spectroscopic data (IR, MS, 1H NMR, 13C NMR, HSQC, HMBC, COSY, and NOESY) and by comparison of the spectral data with those of the related known compounds. The structure of compound 1 is rare due to the presence of a tetrahydropyran ring constituted from C2－C6 and oxygen atom. Compound 7 is the first discovery of eunicellin-based dimer.
The anti-inflammation activity of twelve compounds (1–6 and 8–13) at 10 μM was studied. The results showed that compound 4 inhibited more than 70% express of elastase released in FMLP/CB-induced human neutrophils .

目 錄
論文審定書 i
致謝 ii
中文摘要 iii
英文摘要 iv
化合物1-7 化學結構 v
化合物8-13 化學結構 vi
圖次 ix
表次 xiv
第一章、緒論
第一節、前言 1
第二節、研究背景及目的 2
第三節、文獻回顧 3
第二章、生物材料與研究方法
第一節、Cladiella krempfi 樣品採集時間、地點、分類地位 21
第二節、Cladiella krempfi 的分離流程 22
第三節、實驗設備儀器及材料 25
第三章、化合物之結構證明
第一節、軟珊瑚Cladiella krempfi 所分離出之化合物的結構解析 28
(一)、Krempfielin K (1) 結構之解析 28
(二)、Krempfielin L (2) 結構之解析 39
(三)、Krempfielin M (3) 結構之解析 50
(四)、Krempfielin N (4) 結構之解析 61
(五)、Krempfielin O (5) 結構之解析 72
(六)、Krempfielin P (6) 結構之解析 83
(七)、Krempfielin Q (7) 結構之解析 94
(八)、Litophynins I (8) 結構之解析 107
(九)、Krempfielins A (9) 結構之解析 111
(十)、Krempfielins D (10) 結構之解析 115
(十一)、Sclerophytin F (11) 結構之解析 119
(十二)、Litophynols B (12) 結構之解析 123
(十三)、Litophynols I monoacetate (13) 結構之解析 127
第二節、化合物物理性質及圖譜數據整理 131
第四章、生物活性試驗方法
第一節、發炎活性測試 134
第二節、發炎活性測試結果 136
第五章、結論 137
第六章、參考文獻 141

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