將台灣產踺形羽軟珊瑚Clavularia viridis經過hexane－Acetone、100% Acetone、Acetone－MeOH和100% MeOH粗萃的粗萃物，以乙酸乙酯與水進行partition，取乙酸乙酯層進行矽膠管柱層析，分離純化得七個新的steroids化合物，分別為GN70-153E (1)、GN70-153F (2)、GN70-153I (3)、GN70-272F (4)、GN70-272I (5)、GN70-272K (6)以及GN70-272L (7)；這七個化合物的結構是經由各種光譜分析和HRESI-MS而建立，並且進行了HT-29及P-388癌細胞毒殺活性測試，對HT-29癌細胞株的毒殺活性濃度(ED50值)分別為0.7、0.5、2.9、0.9、2.4、1.0和20.0 microgram/mL，而對P-388癌細胞株的毒殺活性濃度(ED50值)分別為 0.3、0.1、0.4、0.6、1.2、0.7和12.0 microgram/mL。

Seven new steroids, GN70-153E (1), GN70-153F (2), GN70-153I (3), GN70-272F (4), GN70-272I (5), GN70-272L (6), and GN70-272L (7), were isolated from the Formosan soft coral Clavularia viridis by chromatographic separation of the EtOAc layer which was obstained from crude extraction in hexane－Acetone, 100% Acetone, Acetone－MeOH and 100% MeOH then partitioned between EtOAc and H2O. Their structures were determined based on the results of several kinds of spectroscopic analyses and HRESI-MS. Compounds 1-7 exhibited cyctotoxicity against HT-29 and P-388 cell lines in vitro.

目錄
頁次
中文摘要 1
Abstract 2
壹、緒論 3
一、前言 3
二、踺形羽軟珊瑚Clavularia viridis之天然物化學研究回顧 4
三、研究動機 19
貳、踺形羽軟珊瑚Clavularia viridis化學成分之研究 20
一、生物材料採集與鑑定 20
二、踺形羽軟珊瑚Clavularia viridis之分類地位 20
三、踺形羽軟珊瑚化學成分之萃取與分離流程 20
四、踺形羽軟珊瑚化學成分之分離純化 23
1. GN70-153E (1)之分離純化 23
2. GN70-153F (2)之分離純化 26
3. GN70-153I (3)之分離純化 29
4. GN70-272F (4)之分離純化 32
5. GN70-272I (5)之分離純化 35
6. GN70-272I (5)之乙醯化 38
7. GN70-272K (6)之分離純化 41
8. GN70-272L (7)之分離純化 44
五、踺形羽軟珊瑚化學成分之結構解析 47
1. GN70-153E (1)之結構解析 47
2. GN70-153F (2)之結構解析 63
3. GN70-153I (3)之結構解析 79
4. GN70-272F (4)之結構解析 96
5. GN70-272I (5)之結構解析 119
6. GN70-272IA (5A)之結構解析 134
7. GN70-272K (6)之結構解析 139
8. GN70-272L (7)之結構解析 154
參、踺形羽軟珊瑚之生物活性 169
肆、結論 171
伍、參考文獻 173
陸、附錄 179

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