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博碩士論文 etd-0901103-151607 詳細資訊
Title page for etd-0901103-151607
論文名稱
Title
沙門氏菌PCR檢驗技術之開發與研究
Development and Research of PCR Diagnostic Technique to Detect Salmonella
系所名稱
Department
畢業學年期
Year, semester
語文別
Language
學位類別
Degree
頁數
Number of pages
60
研究生
Author
指導教授
Advisor
召集委員
Convenor
口試委員
Advisory Committee
口試日期
Date of Exam
2003-07-05
繳交日期
Date of Submission
2003-09-01
關鍵字
Keywords
沙門氏菌PCR
Salmonella PCR
統計
Statistics
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The thesis/dissertation has been browsed 5687 times, has been downloaded 6294 times.
中文摘要
中文摘要
沙門氏桿菌屬中的傷寒型、敗血型、及腸炎型菌株多達2000餘種血清型,長久以來困擾著微生物檢驗的判別,且不易與其他食物中毒細菌區別。本研究選擇以聚合酶連鎖反應法(PCR法),來進行沙門氏菌分子檢驗技術的開發。我們在沙門氏菌的基因庫中挑選出一個侵入性因子的基因invA做為標的基因,並設計一組PCR引子進行測試,結果顯示在特定primer條件控制下,其他類似菌株(如Shigella , Yersinia, Serratia, Enterobacter , Vibrio ,E. coli)僅在anneal 溫度56∼66℃時能表現顯性。高於69℃時僅有沙門氏桿菌能被偵測的出來。本研究結果有潛力開發為檢驗食品或醫院檢體診斷時偵測Salmonella 菌株的快速方法。未來可進一步配合multi primer PCR技術進一步將沙門氏桿菌傷寒型、敗血型、及腸炎型個別辨識,並提高檢測精確度,或可開發為快速檢測的生物晶片。

Abstract
ABSTRACT

There are more than 2000 serotypes in the typhoid, septic and enteritidis Salmonella strains. This has long been a perplexity for the differentiation of Salmonella and discrimination from other food poisoning bacteria. In this research, we used polymerase chain reaction method (PCR) to develop a molecular technique for the detection of Salmonella. An invasion factor invA gene had been used to design the PCR primers. The results showed that under specific PCR conditions, many related bacterias such as Shigella, Yersinia, Serratia, Enterobacter, Vibrio and E. coli could be detected only with the primer annealing temperature ranging from 56℃~66℃. However, all tested Salmonella strains could be detected above 69℃. This is potential of being developed into a rapid method for detecting Salmonella strains in food or clinical specimens. In the future, it can be further increased the accuracy of detection in coordination with multi-primer PCR technique or developed into a biochip for the detection of Salmonella.

目次 Table of Contents
【目錄】
中文摘要 2
英文摘要 3
前言 4-15
材料與方法 16-20
結果 21-23
討論 24-30
參考文獻 31-42
圖表 43-55
參考文獻 References
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