Title page for etd-0901103-151607


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URN etd-0901103-151607
Author YEOU-HUN CHIANG
Author's Email Address m8821327@student.nsysu.edu.tw
Statistics This thesis had been viewed 4644 times. Download 4568 times.
Department Biological Sciences
Year 2002
Semester 2
Degree Master
Type of Document
Language zh-TW.Big5 Chinese
Title Development and Research of PCR Diagnostic Technique to Detect Salmonella
Date of Defense 2003-07-05
Page Count 60
Keyword
  • Salmonella PCR
  • Abstract ABSTRACT
    There are more than 2000 serotypes in the typhoid, septic and enteritidis Salmonella strains. This has long been a perplexity for the differentiation of Salmonella and discrimination from other food poisoning bacteria. In this research, we used polymerase chain reaction method (PCR) to develop a molecular technique for the detection of Salmonella. An invasion factor invA gene had been used to design the PCR primers. The results showed that under specific PCR conditions, many related bacterias such as Shigella, Yersinia, Serratia, Enterobacter, Vibrio and E. coli could be detected only with the primer annealing temperature ranging from 56℃~66℃. However, all tested Salmonella strains could be detected above 69℃. This is potential of being developed into a rapid method for detecting Salmonella strains in food or clinical specimens. In the future, it can be further increased the accuracy of detection in coordination with multi-primer PCR technique or developed into a biochip for the detection of Salmonella.
    Advisory Committee
  • NONE - chair
  • NONE - co-chair
  • JONG-KANG LIU - advisor
  • Files
  • etd-0901103-151607.pdf
  • indicate access worldwide
    Date of Submission 2003-09-01

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