本實驗利用Nile Red 染色觀察，探討Chlamydomonas reinhardtii CC 400不同環境因子(溫度、鹽度、光和醋酸鹽)對於缺氮誘導油脂累積之影響。以HS medium (High Salt medium) 之NH4Cl 為100% N (9.4 mM)，在0% N 有最大量油脂，於對數生長中期(mid-log phase)之缺氮處理下亦有相同結果。低溫(15oC)下對數生長中期之缺氮處理會抑制缺氮油脂累積，提高光照強度至300 μmol photons • m-2 • s-1亦會抑制累積，黑暗顯著抑制缺氮所誘導之油脂，100 mM NaCl添加促進缺氮誘導之油脂累積，鹽度提高至200 mM NaCl 處理下則抑制累積。缺乏醋酸鹽有顯著抑制，提高醋酸鹽添加量不影響油脂累積量。總而言之，目前結果指出光和醋酸鹽為缺氮誘導油脂累積之必要因子，必須兩者皆提供下才可使缺氮誘導累積油脂達最大量。

This study was to determine the effects of several selected environmental factors (temperature, salinity, light intensity, and acetate) on the nitrogen starvation induced lipid accumulation in Chlamydomonas reinhardtii CC 400 by the Nile Red staining of lipid in the cells. Nitrogen starvation induced lipid accumulation, the extent of lipid accumulation increased as nitrogen concentrations in the medium decreased. For the 9.4 mM NH4Cl of HS medium as 100% N, the absence of NH4Cl from the medium will show the maximum induction in the lipid accumulation. This was also observed in the treatment of algal cells in mid-log phase by the absence of NH4Cl in the medium. A decrease in temperature down to 15oC depressed the nitrogen starvation induction in lipid accumulation for the algal cells from the mid-log phase, while the elevation in the light intensity up to 300 μmol photons • m-2 • s-1 also showed an inhibitory effect. However, the transfer to darkness for the nitrogen starvation also inhibited the lipid increase. The addition of 100 mM NaCl enhanced the nitrogen starvation induced lipid accumulation but the NaCl level up to 200 mM inhibited the increment. The nitrogen starvation induction of lipid increase was partly inhibited due to the absence of acetate, whereas the increase in acetate concentrations in the medium did not have effect on lipid accumulation as compared to normal acetate addition in the medium. Overall, the results of the present study show that light and acetate are essential factors for the maximum lipid accumulation in C. reinhardtii by nitrogen starvation.

中文摘要 ii
英文摘要 iii
目錄 iv
表目錄 v
圖目錄 vi
附錄目錄 vii
縮寫字對照 viii
一、前言 1
二、實驗目的與策略 15
三、實驗架構 16
四、材料與方法 17
五、結果 23
六、討論 32
七、參考文獻 35
八、表 41
九、圖 45
十、附錄 56

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