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博碩士論文 etd-0901111-005212 詳細資訊
Title page for etd-0901111-005212
論文名稱
Title
吳郭魚神經幹細胞特性的探討
The characteristics of Neural stem cell cultured from the tilapia, Oreochromis mossambicus.
系所名稱
Department
畢業學年期
Year, semester
語文別
Language
學位類別
Degree
頁數
Number of pages
51
研究生
Author
指導教授
Advisor
召集委員
Convenor
口試委員
Advisory Committee
口試日期
Date of Exam
2011-06-20
繳交日期
Date of Submission
2011-09-01
關鍵字
Keywords
纖維細胞生長因子、溫度、吳郭魚、神經幹細胞、增生、表皮生長因子
neurosphere, temperature, EGF, tilapia, bFGF, proliferation
統計
Statistics
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中文摘要
吳郭魚 (Tilapia, Oreochromis mossambicus)的腦部構造和功能,與脊椎動物一樣,具有兩性相 (sexual dimorphism)的雌雄差異。性別分化分為腦的性別分化與性腺的性別分化。腦的性別分化 (brain sexual differentiation)與神經發育相關。本研究以細胞培養系統進行,實驗以雄性吳郭魚腦的神經細胞,進行神經幹細胞球(neurosphere)的培養,是為初代培養 (primary culture),並且進行繼代培養 (subculture)。利用上述細胞培養系統,探討初代培養和繼代培養所得neurosphere的生長特性之異同,並探討神經生長相關因子,表皮生長因子 (epidermal growth factor, EGF) 、纖維細胞生長因子 (fibroblast growth factor, FGF),及溫度對其影響之異同。結果顯示,初代及繼代培養之neurosphere,均存在具神經巢蛋白免疫反應 (nestin-immunoreactive),及具分化功能的細胞。研究發現繼代培養之neurosphere直徑顯著大於初代培養。另,FGF與溫度可增加初級培養之neurosphere的細胞球徑。
Abstract
The structure and function of brain shows sexual dimorphism in the vertebrates. Sexual differentiation is divided into brain sexual differentiation and gonad sexual differentiation. Brain sexual differentiation is resulted from the neural development. In the present study, the neurosphere cloned from tilapia, Oreochromis mossambicus, was used. The characteristics of neurosphere derived from both primary- and sub- culture, were studied. The effects of epidermal growth factor (EGF), basic fibroblast growth factor (bFGF, FGF2), and temperature on the neurosphere cloned from both primary culture and subculture, were investigated. These results show that the neurospheres, cloned form both primary- and sub- culture, is consist of the nestin-immunoreactive cell. Furthermore, the cell of the neurosphere shows an ability of differentiation. And the diameter of neurosphere in the subculture is significantly larger than that of primary culture. On the other hand, both FGF and temperature have an effect to increase the diameter of neurosphere in the primary culture.
目次 Table of Contents
致謝……………………………………………………………………i
中文摘要………………………………………………………………ii
英文摘要………………………………………………………………iii
目錄……………………………………………………………………iv
圖次……………………………………………………………………v
前言……………………………………………………………………1
材料與方法……………………………………………………………3
結果……………………………………………………………………12
討論……………………………………………………………………17
參考文獻………………………………………………………………19
圖次……………………………………………………………………24
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