三株從高雄糖廠及堆肥中篩出的嗜高溫好氧菌Geobacillus thermoleovorans T4、Bacillus subtilis b5和Bacillus licheniformis b6，都可以在含有纖維素的培養液中生長。本實驗發現G. thermoleovorans T4則同時具有cellulases和xylanase酵素活性；是本種細菌首先被報導具有纖維素分解能力的菌株，其endoglucanase可作用溫度和酸鹼度範圍分別在30-100℃和pH 4.0-8.0；β-glucosidase可作用溫度和酸鹼度範圍分別在40-90℃和pH 5.0-9.0；exoglucanase可作用溫度在30-90℃；xylanase可作用溫度範圍在30-90℃。如與G. thermoleovorans K-3d的xylanase相比較；可作用溫度範圍相同，但可作用酸鹼值範圍比之更大，為pH 3.0-10.0之間，具有工業應用的價值。而B. subtilis b5和B. licheniformis b6亦具有cellulase的活性，且具有耐高溫的特質。B. subtilis b5的endoglucanase在100℃下1小時的熱穩定性仍保有60％的活性，比所有已知Bacillus屬的熱穩定性都要高，可作用酸鹼值為pH 4.0-7.5之間。B. licheniformis b6的endoglucanase其熱穩定性是在60℃下1小時仍然非常穩定，但隨著溫度上升，酵素的穩定性就越差；可作用酸鹼值為pH 4.0-9.0之間。

Three thermophilic aerobic bacterial strain, Geobacillus thermoleovorans T4, Bacillus subtilis b5 and Bacillus licheniformis b6 were isolated from the wastewater of a Taiwan Sugar Company sugar refinery in Kaohsiung. All strains were capable of growth in cellulose containing medium. We found out that G. thermoleovorans T4 possessed both of the activity of cellulses and xylanase. This was the first report ever in this bacterial spices. Its endoglucanase was active at temperatures ranging from 30℃ to 100℃, and pH ranging from 4.0 and 8.0. Its β-glucosidase activities could be measured at 40℃ - 90℃ and pH 5.0 - 9.0. Its xylanase was active from 30℃ to 90℃ and pH from 3.0 to 10.0, while the exoglucanase was active at temperatures from 30℃ to 90℃. The pH values of xylasnase was better than G. thermoleovorans K-3d. This enzyme was worth of possible in industry application. Both of B. subtilis b5 and B. licheniformis b6 contained a thermoactive endoglucanase. The endoglucanase of B. subtilis b5 was active at pH values between 4.0 and 7.5, and was thermostable at 100℃ for at least 1 hour ( retaining 60% of the original activity ). The stability of this enzyme was better than all known enzymes extracted from Bacillus spices. The endoglucanase of B. licheniformis b6 was active at pH values between 4.0 and 9.0, and thermostable at 60℃ for least 1 hour.

目錄
中文摘要………………………………………………………………Ⅰ
英文摘要………………………………………………………………Ⅱ
前言…………………………………………………………………… 1
實驗目的………………………………………………………………20
材料與方法……………………………………………………………21
結果……………………………………………………………………28
討論……………………………………………………………………37
結論與建議……………………………………………………………41
參考文獻………………………………………………………………43

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