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博碩士論文 etd-0903112-035623 詳細資訊
Title page for etd-0903112-035623
論文名稱
Title
生醫用漸逝波光纖酸鹼值感應器之研究
Evanescent wave fiber optic PH sensor in Biomedical Application
系所名稱
Department
畢業學年期
Year, semester
語文別
Language
學位類別
Degree
頁數
Number of pages
55
研究生
Author
指導教授
Advisor
召集委員
Convenor
口試委員
Advisory Committee
口試日期
Date of Exam
2012-06-22
繳交日期
Date of Submission
2012-09-03
關鍵字
Keywords
塑膠光纖、酸鹼值、SOL-GEL
plastic optical fiber, pH, the SOL-GEL
統計
Statistics
本論文已被瀏覽 5658 次,被下載 559
The thesis/dissertation has been browsed 5658 times, has been downloaded 559 times.
中文摘要
現代人越來越重視健康,追求高品質的生活,因此生醫感測為現代主要發展科技之一,一般人要了解目前健康狀況,除了目前大家熟知的血糖和血壓計外,對於能及時追蹤健康狀況的儀器明顯的過於不足,通常都是要去大醫院檢查,但這卻相當費時,輕則浪費時間,重則可能已經過了治療的黃金時間,因此即時的生醫光纖感測來做為研究方向。本篇是以漸逝波方式來做為我研究方向,利用對我們的感測物專一的染劑,在感測情況下染劑會在不同情況下會有吸收改變情形,使用溶膠凝膠法(Sol-Gel) 的方式來包覆我們專一性染劑,來製作我們的纖衣(cladding),當光在光纖中傳輸時,經由我們專一性的染劑,來
改變光在光纖中傳輸能量來製作生醫感測器。本研究使用一般商用Mitsubishi ESKA CK-40 POF 直徑為 1mm , NA = 0.5。因此耦光簡單,可使架構簡單化且便宜,相較於一般石英光纖可繞度更高且可靠度更好,對光源需求度低,不需用雷射光源,使用一般市售LED 即可, 使用Sol-Gel 技術製作出的多孔隙薄膜包覆我們專一性的染劑操作溫度低,使得製備工程易於控制,以及使用U-bend 方式來增加渐逝波的深度,藉此提高我們感測能力。
Abstract
People to feel more and more attention to health, the pursuit of high quality of life, biomedical sensing is one of the development of technology, person to understand the current state of health, currently known as blood sugar and blood pressure monitors, to track health status
of the instrument is obvious too inadequate, usually go to the hospital for examination, but it is quite time-consuming, ranging from a waste of time, so the immediate biomedical optical fiber sensor to do direction. Evanescent wave ways to do research for my use of the sensing material-specific dye, sensing agent in different situations will be absorbed to change the situation, the use of sol-gel method (Sol-Gel) to
coated with the specific dye, to make our fiber clothes (cladding), when the light in optical fibers, through the specific dye to change the energy of light in optical fibers.
目次 Table of Contents
中文摘要 III
英文摘要 IV
誌 謝 V
第一章 緒論 1
1.1前言........................................................................................1
1.2 尿液檢測.................................................................................8
1.2.1尿液試紙PH感測原理.....................................................10
1.2.2尿液酸鹼值........................................................................11
1.3尿酸鹼度(PH)臨床意義..................................................12
第二章
2.1 溶膠凝膠法技術背景.........................................................14
2.2 Sol-gel 製程原理..................................................................15
2.2.1水解反應..............................................................................15
2.2.2縮合反應..............................................................................16
2.2.3乾燥控制化學添加劑(DCCA)...........................................17
2.2.4 PH 值對溶膠-凝膠法的影響...........................................18
第三章 理論分析
3.1 漸逝波.....................................................................................20
3.2 直形光纖 .............................................................................22
3.3 U-型光纖..............................................................................24
第四章 酸鹼值感測光纖製作及測量
4.1簡介..............................................................................................27
4.2 SOL-GEL製作流程.................................................................27
4.2.1 HPTS-IP ………………………………………….…...…..27
4.2.2染劑滲出實驗.......................................................................28
4.3 塑膠光纖處理...........................................................................29
4.4 SOL-GEL HPTS-IP吸收特性…..............................................30
4.5 光學測量架構...........................................................................32
4.6 PH 感測器之結果.....................................................................33
第五章 結論與未來期望4
5.1 與其他paper相比不同處........................................................39
5.2 須改進以及探討的地方,列出以下幾點。...........................40
5.3 與市售PH計比較....................................................................41
第六章 參考文獻..............................................................................42



圖 目 錄
第一章
圖1-1 酵素電極之反應........................................................3
圖1-2 離子選擇性場效電晶體............................................4
圖1-3 漸逝波 激發型...........................................................7
圖1-4 漸逝波 激發型...........................................................8
第二章
圖2-1 SOL-GEL 反應流程...................................................14
圖2-2(a) 親電性水解反應.........................................................15
圖2-2(b) 親核性水解反應.........................................................16
圖2-3(a) 醇縮合反應.................................................................16
圖2-3(b) 脫水縮合反應.............................................................16
圖2-4 最終產物奈米多孔隙 結構........................................17
圖2-5 Formamide(二甲基甲酰胺)分子結構...........................18
圖2-6 PH對晶粒大小影響......................................................19
圖2-7 酸鹼值對反應速率圖....................................................19
第三章
圖3-1 不同折射率界面上之傳播情形....................................20
圖3-2 不同折射率界面上之傳播情形...................................21
圖3-3 光在光纖傳輸情形.........................................................23
圖3-4 光在U型光纖傳輸情形...............................................24
第四章
圖4-1 HTPS離子對.......................................................................28
圖4-2 HPTS-IP包覆在SOL-GEL..............................................28
圖4-3(a) 圖左 HPTS 圖右HPTS-IP包覆在SOL-GEL中,在DI WATER 浸泡3天。....................................................28
圖4-3(b) 圖左 浸泡3天後SOL-GEL外觀 ,圖右 OM *500 SOL-GEL表面..............................................................28
圖4-4 實驗流程圖.........................................................................30
圖4-5 U-4100 UV-Visible-NIR.......................................................30
圖4-6 實驗PH(2.5.6.7.8) 莫爾濃度 M...........................31
圖4-7 實驗 PH(2.5.6.7.8) 在sol-gel HPTS-IP 吸收..................32
圖4-8 量測系統示意圖.................................................................32
圖4-9 Straight fiber 傳輸功率.......................................................33
圖4-10 U-SHAPED (曲率半徑 5mm) 傳輸功率.........................33
圖4-11 Straight fiber 在465nm不同PH下功率.........................34
圖4-12 U-SHAPED 在465nm不同PH下功率..........................34
圖4-13 Straight fiber 在465nm不同PH下功率PH 5~8..................35
圖4-14 U-SHAPED 在465nm不同PH下功率PH 5~8....................35

表 目 錄
表1-1 目前市售尿液試紙.....................................................................10
表4-1使用藥劑 ....................................................................................36
表4-2光纖規格......................................................................................36
表4-3化學藥品......................................................................................37
表4-4儀器與光學零組件......................................................................38
表5-1市售PH 比較...............................................................................41
參考文獻 References
Sai, V., T. Kundu, and S. Mukherji, Novel U-bent fiber optic probe for
localized surface plasmon resonance based biosensor. Biosensors and
Bioelectronics, 2009. 24(9): p. 2804-2809.
2. Lee, S.T., et al., A sensitive fibre optic pH sensor using multiple sol-gel
coatings. Journal of Optics A: Pure and Applied Optics, 2001. 3: p. 355.
3. Dong, S., et al., Broad range pH sensor based on sol-gel entrapped indicators
on fibre optic. Sensors and Actuators B: Chemical, 2008. 129(1): p. 94-98.
4. Rayss, J. and G. Sudolski, Ion adsorption in the porous sol-gel silica layer in
the fibre optic pH sensor. Sensors and Actuators B: Chemical, 2002. 87(3): p.
397-405.
5. Armin, A., M. Soltanolkotabi, and P. Feizollah, On the pH and concentration
response of an evanescent field absorption sensor using a coiled-shape plastic
optical fiber. Sensors and Actuators, A: Physical, 2011. 165(2): p. 181-184.
6. Belhadj Miled, O., H. Ben Ouada, and J. Livage, pH sensor based on a
detection sol–gel layer onto optical fiber. Materials Science and Engineering:
C, 2002. 21(1): p. 183-188.
7. Wu, H.Y., Development of Fiber-Optic Based Multi-Functional Biochemical
Sensors for Blood Glucose Monitoring. 2002.
8. Matsuo, H., et al., Evanescent wave optical fiber sensor using adsorption LB
films. Synthetic metals, 2000. 115(1): p. 37-39.
9. Preejith, P., et al., Total protein measurement using a fiber-optic evanescent
wave-based biosensor. Biotechnology letters, 2003. 25(2): p. 105-110.
10. Ou, I., Co-Encapsulation of VHb fused D-amino acid oxidase and catalase in
sol-gel silica glass. 2006.
11. Hench, L.L. and G.F. Orcel, Drying control chemical additives for rapid
production of large sol-gel derived silicon, boron and sodium containing
monoliths, 1989, Google Patents.
12. 柳召永, et al., 溶胶凝胶法 N, N-二甲基甲酰胺对多孔炭孔隙结构的影响.
化工进展, 2007. 26(9): p. 1316-1318.
13. Juo, J.Y., The influences of different sol-gel matrices for the preparation of
monolithic column applied to capillary electrochromatography. 2004.
14. Dakin, J. and B. Culshaw, Optical fiber sensors: Principles and components.
Volume 1. Boston, MA, Artech House, 1988, 343 p. For individual items see
A90-36770 to A90-36775., 1988. 1.
15. Snyder, A.W. and J.D. Love, Optical waveguide theory. Vol. 190. 1983:
Springer.
43
16. Khijwania, S. and B. Gupta, Fiber optic evanescent field absorption sensor:
effect of fiber parameters and geometry of the probe. Optical and quantum
electronics, 1999. 31(8): p. 625-636.
17. Wencel, D., B. MacCraith, and C. McDonagh, High performance optical
ratiometric sol-gel-based pH sensor. Sensors and Actuators B: Chemical, 2009.
139(1): p. 208-213.
18. 洪明哲, 以溶膠-凝膠法製作光纖感測器量測pH 值 H 碩士畢業論文, 大
同大學, 2007
19. 陳正豪“光纖漸逝波奈米金粒子表面電漿增強式生物分子感測器纖漸碩
士畢業論文, 國立陽明大學, 2005
20. http://zh.wikipedia.org/wiki/%E7%94%B2%E9%85%B0%E8%83%BA
維基百科
21. 詹佳樺 溶膠-凝膠法製備聚甲基丙烯酸甲酯 / 二氧化矽混成體之研究,
國立中央大學畢業論文,2001
22. 林世仁利用放電紡絲法開發 聚乙烯醇縮丁醛及二氧化矽奈米纖維國立
成功大學畢業論文.2007
23. 物理雙月刊(廿八卷四期)2006 年8 月
24 http://www.supermt.com.tw/urinalysis.htm (福島醫學尿液分析)
25 http://www.gout.com.tw/introduce/intro_pink2.htm (泌尿道結石)
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