本研究探討氮限制之貧營養鹽南中國海海域，是否有<10 &micro;m小體型單細胞固氮藍綠藻存在，及其分布與環境因子間之關係。於2004年2月到2005年1月，在7個航次14個不同測站進行採樣並攜回樣品進一步分析。螢光顯微鏡觀察結果，確定水體中有含藻紅素，呈橘黃色螢光的<10 &micro;m單細胞藍綠藻，以（1）反轉錄聚合

This research investigated the existence of <10 μm nitrogen-fixing unicellular cyanobacteria in the South China Sea. The surveys covered the period from February 2004 to January 2005 and a total of seven cruises. The unicellular cyanobacteria that express orange-yellow from cellular phycoerythrin were observed under a fluorescence microscope. Their expressions in nitrogen-fixation were confirmed by the results from reverse transcription polymerase chain reaction (RT-PCR) and whole cell fluorescence immunolocalization of nitrogenase. The nifH gene sequences of the unicellular cyanobacteria collected from the South China Sea was with >90% identities of their nucleotides similar to the nifH gene sequences of unicellular diazotrophs from ALOHA (Hawaii) as well as Synechocystis sp. WH 8501, Cyanothece sp. ATCC 51142, Cyanothece sp. WH 8902, Cyanothece sp. WH 8904 and Synechococcus sp. RF-1. Positive reactions of fluorescence immunolocalization of nitrogenase were only observed in some, not all, of <10 μm unicellular cyanobacteria, suggesting that cell counting alone can not be used to estimate nitrogen fixation rate. There was great seasonal and spatial variation in the unicellular cyanobacteria cell density. There was, however, no significant relationship between cell density and the investigated environmental factors. Cell density was high when temperature was high or where stratification index of water column was high, such as in summer or in basin in contrast to other seasons or the shelf-slope regions.

目錄

章次 頁數
中文摘要...................................................... I
英文摘要...................................................... II
目錄.......................................................... III
表目錄........................................................ IV
圖目錄........................................................ V
附錄目錄...................................................... VII
一、前言...................................................... 1
二、文獻回顧.................................................. 4
三、材料與方法................................................ 16
四、結果...................................................... 27
五、討論...................................................... 41
六、參考文獻.................................................. 45
表............................................................ 51
圖............................................................ 66
附錄.......................................................... 92

參考文獻
（本論文英文文獻採用Deep-Sea Research I格式）
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