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博碩士論文 etd-0905107-233303 詳細資訊
Title page for etd-0905107-233303
論文名稱
Title
溫度對吳郭魚神經細胞發育的影響及其機制的探討
The influence of temperature in the neuronal development of tilapia, Oreochromis mossambicus.
系所名稱
Department
畢業學年期
Year, semester
語文別
Language
學位類別
Degree
頁數
Number of pages
76
研究生
Author
指導教授
Advisor
召集委員
Convenor
口試委員
Advisory Committee
口試日期
Date of Exam
2007-07-27
繳交日期
Date of Submission
2007-09-05
關鍵字
Keywords
莫三比克口孵吳郭魚、神經細胞、溫度、神經發育
neuronal development, Oreochromis mossambicus, temperature, neuron
統計
Statistics
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中文摘要
脊椎動物的腦部性別分化(brain sexual differentiation)造成腦部在構造及功能上具有兩性相(sexual dimorphism),而腦部性別分化源自於腦部神經的發育。神經的發育受生物體本身之基因調控及外在環境的影響,外在環境的影響可分為物理性因子(如:溫度、酸鹼值…等)及化學性因子(如:性類固醇、神經傳導物質…等)。血清素( 5-hydroxytryptamine, 5-HT ) 主要作為神經傳導物質(neurotransmitter)和神經修飾物質(neuromodulator),與神經發育有關。在吳郭魚的性別分化關鍵時期,高溫可促進吳郭魚雄化的比例,低溫促進吳郭魚雌化的比例,說明溫度在性別分化上扮演重要的角色。本研究以雄性莫三比克口孵吳郭魚(Oreochromis mossambicus)下視丘的初代神經細胞培養(Primary Neuron Culture)探討血清素系統及溫度對神經細胞發育的影響與機制。結果說明,血清素1A 受體(5-HT1A receptor)參與血清素誘發神經細胞增生的作用。高溫促進神經細胞的增生,與血清素1A 受體相關。
Abstract
The structure and functions of brain show sexual dimorphism in vertebrates. Brain sexual differentiation is resulted from the neural development. The neural development is determined not only by the genetic regulation, but also by the extrinsic environmental influences. Serotonin (5-hydroxytryptamine, 5-HT ) functions as a neurotransmitter or/and neuromodulator in the central nervous system. Serotonin plays a role in the neural development via serotonin receptors. The sexual differentiation of tilapia is influenced by water temperature. The lower temperature induces a higher proportion of female while the elevated temperature induces a higher proportion of male in tilapia. In the present study, the influence of temperature on the proliferation of the neurons was investigated. These results show that the proliferation of neurons are varied with the temperature. The elevated temperature influences the proliferation of neurons via central serotonin system. Serotonin 1A receptor is involved in the serotonin-induced proliferation of neuron.
目次 Table of Contents
致謝辭 ………-I-
中文摘要 ………-II-
英文摘要 ………-III-
目錄 ………-IV-
附圖(表)目錄 ………-V-
壹、前言(實驗目的) ………-01-
貳、實驗材料與方法 ………-09-
參、結果 ………-25-
肆、討論 ………-36-
伍、參考文獻 ………-40-
陸、附圖(表) ………-48-
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