本研究探討裂片石蓴（Ulva fasciata Delile）protein disulfide isomerase (PDI; EC 5.3.4.1)及 thioredoxin (Trx) 基因在高鹽逆境（90‰ 1 小時）下的基因表現與多元胺（polyamine）如何調控 PDI 及 Trx 基因表現。高鹽造成 H2O2 累積，表示有氧化逆境產生。游離態及蛋白質 SH 含量在高鹽處理下有降低的趨勢，顯示高鹽逆境造成氧化還原趨向氧化態。高鹽下，Trx 基因表現增高暗示裂片石蓴已啟動抗氧化功能，以清除因 ROS 所造成的傷害，PDI 基因表現及活性的增高，則暗示裂片石蓴在高鹽逆境下可能以調整蛋白質構形，而影響其活性與功能。多元胺（1 mM）處理的裂片石蓴在高鹽下，Putrescine 處理不會抑制 H2O2 累積，而 Spermidine 及 Spermine 抑制高鹽所誘導之 H2O2 累積，反應出在多元胺之 spermidine 及 Spermine 處理的 90‰ 之氧化逆境程度下降。Spermidine 及 Spermine 處理下，游離態 SH 含量上升及蛋白質 SH 含量下降，證明 Spermidine 及 Spermine 以增加游離態 SH 含量而減少氧化逆境，同時恢復為還原態，促進 PDI 基因表現量及活性的增加。30‰ 下，Spermine 處理也會增加 PDI 基因表現量及活性，Putrescine 及 Spermine 也會增加 Trx 基因表現量，暗示在正常狀況下多元胺就有參與抗氧化的調節。在植物氧化還原調控，裂片石蓴在高鹽初期會受到氧化逆境的影響，啟動 PDI 和 Trx 基因表現，多元胺處理可以調控高鹽初期 –SH 還原之 PDI 與 Trx 基因表現。

This research has investigated the gene expression of protein disulfide isomerase (PDI; EC 5.3.4.1) and thioredoxin (Trx) in the marine macroalga Ulva fasciata Delile in response to hypersaline (90‰) for 1 h. 90‰ induced H2O2 accumulation, reflecting the occurrence of oxidative stress. The contents of free and protein-bound SH were increased by 90‰. Trx transcripts increased in response to 90‰. PDI transcripts and enzyme activities increased in response to 90‰. H2O2 accumulation under 90‰ condition was increased by putrescine (Put) but decreased by spermidine (Spd) and spermine (Spm). By treatment of spermidine and spermine, the contents of free SH was increased and the contents of protein-bound SH decreased, showing that spermidine and spermine can increase free SH against oxidative stress. The gene expression and activity of PDI were further increased by Spd and Spm. Overall, the gene expression of PDI and Trx were responded to 90‰ for 1 hour and were adjusted protein–SH in polyamines treatment.

謝辭 i
中文摘要 ii
英文摘要 iii
目錄 iv
圖目錄 v
表目錄 vi
一、前言 1
二、材料與方法 8
三、結果 16
四、討論 43
參考文獻 48
附錄 57

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