高雄港廢汙水排放區底棲生物之群聚結構及四種底棲動物蛋白質表現之研究

摘要

本研究探討高雄港廢汙水排放區底棲生物之群聚結構及四種底棲動物蛋白質表現是否受廢汙水排放之影響。分別於2009年12月(冬季)及2010年7月（夏季）在中洲廢汙水處理廠汙水排放區附近3個採樣點，中山大學廢汙水處理廠汙水排放區附近2個採樣點，及其北方2公里處之對照樣點進行底拖網採樣。冬季及夏季之水溫分別為26.8、33.7oC; 鹽度分 別為32.2、31.5‰；酸鹼度分別為8.1、8.0。二季六個測站共捕獲底棲動物5門31科48種；季節間與測站間之環境參數與物種組成無顯著差異。以一維蛋白質電泳檢測三種魚類(Lei-ognathus splendens, Apogon fasciatus, Engyprosopon multisquama）及一種螃蟹（Portunus hastatoides) 之蛋白質表現，此三種魚類及一種螃蟹之蛋白質表現在季節間與測站間亦 無顯著差異。依據相關文獻及本研究結果推測高雄港內存在多元之污染物也包含本研究之六個測站8公里以上, 而造成測站間之底棲生物群聚結構及四種底棲動物蛋白質表現相似。
關鍵字: 一維蛋白質電泳、Leiognathus splendens、 Apogon fasciatus、 Engyprosopon multisquama、Portunus hastatoides

The study of benthic community structure and protein expression pattern of four benthic species in effluent areas of sewage treatment plants in Kaohsiung, Taiwan

Dwi Candra Pratiwi
Advisor: Dr. Li-Lian Liu

Institute of Marine Biology, National Sun Yat-sen University, Kaohsiung, Taiwan, R.O.C.

Abstract
In order to gain better understanding on the impacts of sewage treatment plant (STP) effluent on marine environment, the present study was undertaken to investigate the structure of benthic community and protein expression patterns of 4 benthic species in STP effluent areas in Kaohsiung, Taiwan. Six sites were selected, 3 near Jhong Jhou STP, 2 near NSYSU STP and one reference site. Samples were collected in December 2009 (winter) and July 2010 (summer) by bottom trawling. In winter, temperature was 26.8oC; salinity and pH were 32. 2‰ and 8.1. In summer, environmental data were 33.7oC, 31.5‰ and 8.0, respectively. In all, 5 phyla, 31 families and 48 different species were captured in two seasons. Proteomic approach by one-dimensional sodium dodecyl sulfate polyacrylamide gel electrophoresis (1D SDS-PAGE) was conducted on 3 fishes, Leiognathus splendens, Apogon fasciatus, Engyprosopon multisquama, and one crab Portunus hastatoides. Environment characteristics and structure of benthic community had no significant difference among the 6 sampling sites. Protein expression patterns based on the four examined species also indicated no significant difference among sites. The complexity of pollutants in Kaohsiung Harbor is probably the main reason affecting all sampling sites. In other words, contaminated area in Kaohsiung Harbor may cover a wide range, at least
III
over the present sampling distance, i.e. 8km which causes no difference in community structure and protein expression pattern among control, Jhong Jhou STP and NSYSU STP sites.
Key words: 1D SDS-PAGE, Leiognathus splendens, Apogon fasciatus, Engyprosopon multisquama, Portunus hastatoides

Contents
Abstract in Chinese…………………………………… I
Abstract in English……………………………………. II
Acknowledgment……………………………………… IV
Contents………………………………………….……. V
List of Tables……………………………......………… VI
List of Figures………………………………....………. VII
List of Appendix……………………………………….. VIII
Introduction…………………………………………….. 1
Materials and methods………………………………. 6
Results…….…………………………………………… 9
Discussion…………………………………………… 12
Conclusion………………………………………..….. 16
References…...………………………………………. 17
Tables……………………………………………...…. 22
Figures………………………………..………………. 33
Appendix……………………………………..……….. 48

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