烏龜怪方蟹在龜山島龜首淺海熱泉區域為優勢物種，但目前對於其族群的動態仍然不清楚。本研究利用穩定同位素方法與一維蛋白質電泳，探討烏龜怪方蟹在黃泉與白泉兩種不同棲地的食性及蛋白質表現之差異。研究結果顯示2009年兩棲地的食物來源不同，但2010年則食物來源相似，在不同年度之間有差異。白泉怪方蟹之δ15N值範圍較廣 (2009: 6.09─9.90 ‰; 2010: 4.72─8.94 ‰)，可能與當地較多元的食物來源有關，且浮游動物並非怪方蟹之主要食物來源。採集於相同棲地的烏龜怪方蟹，在肌肉蛋白質表現上有顯著差異，表示在小尺度的範圍內有微棲地適應的現象，且在不同棲地間有遷徙的情形，推測可能存在尚未被發現的非熱泉區棲地。人工馴養12小時的烏龜怪方蟹在蛋白質表現上，與馴養3.5天與24周的烏龜怪方蟹相似度較高，反而與原棲地的烏龜怪方蟹差異較大，顯示烏龜怪方蟹在12小時內即可適應人工給予的新環境。

Xenograpsus testudinatus is a dominant species in shallow hydrothermal vents off Kueishan Island, Taiwan. But we know little about crab populations in vent areas. In this study, stable isotope and one dimension protein expression profiles were studied to evaluate the differences of X. testudinatus from different habitats, i.e. white and yellow vents. Results indicated food sources between the two habitats were different in 2009, but similar in 2010. The ranges of δ15N value of X. testudinatus were broad which might result from abundant food in white vent. Furthermore, zooplankton might not be the major food source of X. testudinatus. Significant difference in protein expression profiles between habitats indicate that they adapt to different habitats in small scales with migration between habitats. And, crabs may have other habitats outside vent areas. After culture in laboratory for 12 hours, crab protein expression profiles were different from their original ones, indicating their acclimation to a new environment is relative fast.

謝辭 I
中文摘要 II
英文摘要 III
目錄 IV
表目錄 V
圖目錄 VI
一、前言 p.1
二、材料與方法 p.5
2.1 採樣地點 p.5
2.2 實驗室馴養 p.5
2.3 蛋白質體分析 p.6
2.3.1 蛋白質萃取與定量 p.6
2.3.2 ㄧ維蛋白質電泳 p.6
2.3.3 固定與染色 p.6
2.3.4 膠片分析 p.7
2.4 穩定同位素測定 p.7
三、結果 p.8
四、討論 p.12
五、參考文獻 p.16

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