在水產動物疾病的預防是顯著的經濟意義，因為小魚有較高的死亡率，由於高發病率的疾病，低水的質量等等。幾年前，主要治療，以保護魚類細菌性疾病，針對不同的孵化條件，化療藥物（抗生素）或接種疫苗。以前的方法可能會改變配置一個健康的腸道菌群，而接種方法可壓力為魚;兩種方法可以使訪問一些病原體。對化療藥物的使用也可導致耐藥細菌，所以他們的使用應該限制或至少控制，等等，本研究進行調查​​的影響，不同數量的益生菌對水質參數在文化石斑魚魚種（斜帶石斑魚），物種已成為其中的主要養殖魚類在台灣根據糧農組織的。益生菌的影響，枯草芽孢桿菌，對石斑魚魚種養殖和水的質量進行了評估，本研究通過添加益生菌的飼料。兩種給藥濃度的益生菌包括0.1％，1.0％和控制。在實驗過程中，魚種的發展，增長速度，成活率和水質參數，包括氨氮和亞硝酸鹽氮，菌落總數，計算和推定弧菌進行了測定。推定弧菌數量呈現了顯著抑制芽孢桿菌治療後。

Preventing diseases in aquatic animals is of significant economic importance, since small fish have high mortality rates due to high incidences of disease, low water quality among others. Several years ago, the main treatment to protect fishes against different bacterial diseases in hatchery conditions were chemotherapeutic agents (antibiotics) or vaccination. The previous methods may change the profile of a healthy gut microflora, while the vaccination method can be stressful for fish; both methods can enable the access of some pathogens. The use of chemotherapeutic agents can also lead to resistant bacteria, and so their use should be restricted or at least controlled; and so, this study was conducted to investigate the effects of different amounts of probiotics on the water quality parameters in the culture of grouper fingerling (Epinephelus Coioides); species which has become one of the main cultured fish in Taiwan according to FAO. The effects of the probiotic, Bacillus subtilis, on grouper fingerling breeding and water quality was evaluated in this study by adding the probiotic to the feed. Two concentrations of probiotic were administered including 0.1%, 1.0% and the control. During the experiment, fingerling development, growth rate, survival rate and water quality parameters including ammonia-N and nitrite-N, total bacterial count, and presumptive Vibrio count were determined. The presumptive Vibrio count presented was significantly suppressed after Bacillus treatment.

Table of Contents
Pages
Acknowledgement………………………………..… I
Abstract………………………...…………………….. II
Table of Contents……………………...……………. III
List of Tables………………………………………… IV
List of Figures……………….……………………….. V
Chapter 1 Introduction………………………………. 1
Chapter 2 Materials and methods…...…………….. 10
2.1 Experimental Design…………………………..... 10
2.2 Fish Performance……………..…………………. 11
2.3 Recirculatory System............................................. 11
2.4 Bacterial Counts…………....…………………….. 12
2.5 Water Quality Analysis…………………………… 12
2.6 Analysis of Data..…………………………..……... 13
Chapter 3 Results…………………..………………… 14
Chapter 4 Discussion...…………………………...…. 16
Tables……………....……………………………..…… 19
Figures……….................................……………..…….. 22
References………...…………………………………... 30

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