本研究以添加不同濃度花生四烯酸（ARA）飼料飼育平均體重約16g的點帶石斑稚魚，探討飼料添加ARA對石斑魚成長表現與免疫反應的影響。試驗飼料以酪蛋白為蛋白質源，含油脂9 g/100 g diet，以鱈魚肝油和花生四烯酸油調整比例，得到ARA的濃度分別為0、0.2、0.4、0.6、0.8和1.0 g/100g diet六種飼料。經十二週飼育試驗，發現ARA添加並不顯著影響石斑魚之增重，而0.6 %組及0.8 %組平均增重率高於其他各組，石斑魚肝臟ARA含量和ARA/EPA及ARA/DHA比例隨飼料ARA含量提高而增加，飼料添加ARA顯著（p<0.05）提升石斑魚的免疫活性：ARA添加顯著提升試驗魚溶菌&#37238;活性，ARA添加量越高，溶菌&#37238;活性顯著增高；ARA添加也顯著提高石斑魚頭腎之呼吸爆活性，攝取ARA 0.6 %之飼料組的試驗魚呼吸爆活性顯著高於其他組別，攝取ARA 0.6 %及0.8 %ARA之飼料組的試驗魚白血球增生顯著高於其他組別，攝取ARA 0.6 %之飼料組的試驗魚吞噬活性顯著高於其他組別，而攝取ARA 0.8 %之飼料組的試驗魚細胞激素表現量IL-1β和TNF-α顯著高於其他組別。本研究結果顯示飼料ARA含量對點帶石斑幼魚成長無顯著影響，但會影響魚體免疫反應，當添加量為0.6-0.8 %魚體多項免疫反應顯著高於其他添加量。

This research studied the dietary effect of arachidonic acid (ARA) on growth performance and immune responses in juvenile grouper (Epinephelus coioides). The test diets used casein as protein source, and contained 9 g/100g lipid. Cod liver oil and arachidonic acid oil were used to adjust the ARA levels to 0, 0.2, 0.4, 0.6, 0.6, 0.8, and 1.0 g/100 g diet in the 5 test diets, respectively. Result of 12-week feeding trial show that the ARA supplementation had no significantly effect on the weight gain of the grouper, although 0.6 % and 0.8% groups showed higher weight gains than other groups. Liver fatty acid composition of the groupers was affected by dietary ARA. Liver ARA concentration, ARA/EPA and ARA/DHA increased with increasing dietary ARA levels. The immune responses was affected significantly by the dietary treatments. Dietary supplementation of ARA significantly enhanced plasma lysozyme activity. Lysozyme activity was increased with increasing dietary ARA levels. Dietary ARA significantly also enhanced head-kidney leucocytes respiratory burst activity. The respiratory burst activity of the 0.6 % ARA group was significantly higher than the other groups. Dietary ARA significantly enhanced head-kidney leucocyte proliferation and the dosages of 0.6 % and 0.8 % ARA were significantly more effective than the other groups. The blood phagocytic activity of the 0.6 % ARA was significantly higher than the other groups. The blood cytokine IL-1β and TNF-α was significantly higher in the 0.8 % ARA group than the other groups. This research shows that feeding the grouper diets cointaining ARA at an optimal level could significantly increase immune responses of the fish.

中文摘要……………………………………………………………..………………Ⅰ
英文摘要…………………………………………………………………………..…Ⅱ
目錄…………………………………………………………….………….…………IV
表目錄…………………………………………………….……………………..……V
圖目錄………………………………………………………………………….….. VII
附錄目錄…...…………………………………………………………...…….……VIII
文獻回顧………………………………………………………………………..……..1
前言…………………………………………………………………………………..17
材料與方法…………………………………………………………………………..18
結果…………………………………………………………………………………..40
討論…………………………………………………………………………………..53
參考文獻……………………………………………………………………………..61
附錄…………………………………………………………………………………..84

吳豐成，2002。瑪拉巴石斑稚魚之必需脂肪酸營養及其對免疫反應之影響。國立中山大學海洋生物研究所博士論文。
呂明毅，1998。石斑魚的繁養殖 (三)。漁業週訊，627: 3。
徐崇哲，1997。養殖石斑類虹彩病毒之分離、特性及病原性相關之研究。國立台灣海洋大學水產養殖研究所碩士論文。
蘇偉銘，1999。病原性弧菌其細胞外產物與石斑魚血清成份之交互作用。國立台灣海洋大學水產養殖研究所碩士論文。
陳威志，2006。以亞麻仁油和玉米油取代魚油對海鱺幼魚成長、脂肪酸代謝及免疫反應的影響。國立中山大學海洋生物研究所碩士論文。
林佩貞，2006。飼料添加植物油脂對海鱺稚魚成長、脂肪狀態、代謝酵素活性、及脂肪酸組成的影響。國立中山大學海洋生物研究所碩士論文。
王涵，2005。核&#33527;酸對石斑稚魚成長與免疫之影響。國立台灣海洋大學食品科學系碩士論文。
荻野珍吉，1986。魚類之營養與飼料。國立編譯館。
AOAC (Association of Official Analytic Chemists). 1984. Official Methods of Analysis. 13th edition. Washington, D.C., USA.
Aoki, T. 1992. Chemotherapy and drug resistance in fish farms in Japan. In: Shariff, M., Subasighe, R. P., Arthur, J. R. (Eds.), Disease in Asian Aquaculture 1. Fish Health Section, Asian Fisheries Society, Manila, Philippines, 519-529.
Ayorinde, F. O., J. Jrcliffon, O. A. Afolabl and R. I. Shepard. 1988. Rapid transesterification and mass spectrometric approach to seed oil anaysis. Journal of American Oil Chemistry Society 65: 942–948.
Bagga, D., L. Wang, R. Farias-Eisner, J. A. Glaspy and S. T. Reddy. 2003. Differential effects of prostaglandin derived from omega-6 and omega-3 polyunsaturated fatty acids on COX-2 expression and IL-6 secretion. Proceedings of the National Academy of Sciences of the United states of America 100:1751-1756.
Bailie, M. B., T. J. Standiford, L. L. Laichalk, M. J. Coffey, R. Strieter and M. Peters-Golden. 1996. Leukotriene-deficient mice manifest enhanced lethality from Klebseilla pneumonia in association with decreased alveolar macrophage phagocytic and bactericidal activities. Journal of Immunology 157:5221-5224.
Bechous, S., M. Dubois, G. Nemoz, M. Lagarde, and A. F. Prigent. 1998. Docosahexaenoic acid lowers phosphatidate level in human activated lymphocytes despite phospholipidase D activation. Journal of Lipid Research 39: 873-883.
Bell, M. V., C. M. F. Simpson and J. R. Sargent. 1983. N-3 and n-6 polyunsaturated fatty acids in the phosphoglycerides of salt-secreting epithelia from two marine fish species. Lipids 18:720-726.
Bell, M. V. and D. R. Tocher. 1989. Molecular species composition of the major phospholipids in brain and retina from rainbow trout (Salmo gairdneri). Occurrence of high levels of di-(n-3) polyunsaturated fatty acid species. Biochemical Journal 264: 909-915.
Bell, M.V. and J. R. Dick. 1990. Molecular species composition of phosphatidylinositol from the brain, retina, liver and muscle of cod (gadus morhua). Lipids 25: 691-694.
Bell, J. G., A. H. McVicar, M. T. Park and J. R. Sargent. 1991. High dietary linoleic acid affects fatty acid compositions of individual phospholipids from tissues of Atlantic salmon (Salmo salar): association with stress susceptibility and cardiac lesion. Lipids 121:1163-1172.
Bell, J. G., J. R. Dick, A. H. McVicar, J. R. Sargent and K. D. Thompson. 1993. Dietary sunflower, linseed and fish oils affect phospholipid fatty acid composition, development of cardiac lesions, phospholipase activity and eicosanoid production in Atlantic salmon (Salmo salar). Prostaglandins, Leukotrienes and Essential Fatty Acids 49: 665-673.
Bell, J. G., J. R. Dick, J. R. Sargent and A. H. McVicar. 1992. Dietary linoleic acid affects phospholipid fatty acid composition in heart and eicosanoid production by cardiomyocytes from Atlantic salmon (Salmo salar). Comparative Biochemistry and Physiology 103:337-342.
Bell, J. G., D. R. Tocher, B. M. Farndale, D. I. Cox, R. W. McKinney and J. R. Sargent. 1997a. The effect of dietary lipid on polyunsaturated fatty acid metabolism in Atlantic salmon (Salmo salar) undergoing parr-smolt transformation. Lipids 32: 515-525.
Bell, J. G., B. M. Farndale, M. P. Bruce, J. M. Navas and M. Carrillo. 1997b. Effects of broodstock dietary lipid on fatty acid compositions of eggs from sea bass (Dicentrarchus labrax). Aquaculture 149:107- 119.
Bell, J. G., J. McEvoy, D. R. Tocher, F. McGhee, P. J. Campbell and J. R. Sargent. 2001. Replacement of fish oil with rapeseed oil in diets of Atlantic salmon (Salmo salar) affects tissue lipid compositions and hepatocyte fatty acid metabolism. The Journal of Nutrition 131:1535-1543.
Bell, J. G., R.J. Henderson, D. R. Tocher, F. McGhee, J. R. Dick, A. Porter, R. P. Smullen and J. R. Sargent. 2002. Substituting fish oil with crude palm oil in the diet of Atlantic salmon (Salmo salar) affects muscle fatty acid compositions and hepatic fatty acid metabolism. Journal of Nutrition 132:222-230.
Bell, J. R. and J. R. Sargent. 2003. Araconic acid in aquaculture feeds: current status and future opportunities. Aquaculture 218:491-499.
Bell, M. V., R. S. Batty, J. R. Dick, K. Fretwell, J. C. Navarro and J. R. Sargent. 1995. Dietary deficiency of docosahexaenoic acid impairs vision at low light intensities in juvenile herring (Clupea harengus L.). Lipids 30:373-476.
Bell, M. V., R. J. Henderson, B. J. S. Pirie and J. R. Sargent. 1985a. Effects of dietary polyunsaturated fatty acid deficiencies on mortality, growth and gill structure in the turbot (Scophthalmus maximus Linnaeus). Journal of Fish Biology 26:181-191.
Bell, M.V., R. J. Henderson and J. R. Sargent. 1985b. Changes in the fatty acid composition of phospholipids from turbot (Scophthalmus maximus) in relation to dietary polyunsaturated fatty acid deficiencies. Comparative Biochemistry and Physiology 81: 193-198.
Bell, J. G., I. Ashton, C. J. Secombes, B. R. Weitzel, J. R. Dick, and J. R. Sargent. 1996a. Dietary lipid affects phospholipid fatty acid compositions, eicosanoid production and immune function in Atlantic salmon (Salmo salar). Prostaglandins, Leukotrienes and Essential Fatty Acids 54:173-182.
Bell, M. V., J. R. Dick, M. Thrush and J. C Navarro. 1996b. Decreased 20:4n-6/20:5n-3 ratio in sperm from cultured sea bass, Dicentrarchus labrax, broodstock compared with wild fish. Aquaculture 144:189-199.
Belton, O. and D. J. Fitzgerald. 2003. Cyclooxygenase isoforms and atherosclerosis. Expert Reviews in Molecular Medicine 5:1-18.
Bessonart, M., M. S. Izquierdo, M. Salhi,C. M. Hernandez-Cruz, M. M. Gonzalez and H. Fernandez-Palacios. 1999. Effect of dietary arachidonic acid levels on growth and fatty acid composition of gilthead sea bream (Sparus aurata L.) larvae. Aquaculture 179:265-275.
Bitman, J., L. Wood, N. R. Melta, P. Hamosh, and M. Hamosh. 1984. Composition of phospholipid composition of breast milk from mothers of term and pretern infants during lactation. American Journal Clinical Nutrition 40:1103-1119.
Blazer, V. S. 1991. Piscine macrophage function and nutritional influences: a review. Journal of Aquatic Animal Health 3: 77-86.
Borda, E. S., A. Tenenbaum, M. E. Sales, L. Rumi and L. Sterin-Borda. 1998. Role of arachidonic acid metabolites in the action of a beta-adrenergic agonist on Human monocyte phagocytosis. Essential of Fatty Acids 58: 85-90.
Braun-Nesje, R., K. Bertheussen, G. Kaplan and R. Seljelid. 1981. Salmonid macrophages: separation, in vitro culture and characterization. Journal of Fish Diseases 4: 141-151.
Brenner, R. R., H. Garda, I. N. de Gomez Dumm, and H. Pezzano. 1981. Early effects of EFA deficiency on the structure and enzymatic activity of rat liver microsomes. Progress in Lipid Research 20:315-321
Bruce, M., F. Oyen, G. Bell, J. F. Asturiano, B. Farndale, S. Zanuy, J. Ramos, N. Bromage. 1999. Development of broodstock diets for the European sea bass (Dicentrarchus labrax) with special emphasis on the importance of n-3 and n-6 HUFA to reproductive performance. Aquaculture 177: 85-98.
Calder, P. C. 1995. Fatty acids, dietary lipids and lymphocyte functions. Biochemical Society Transactions 23: 302-309.
Calder, P. C., J. A. Bond and E. A. Newsholme. 1989. Fatty acid inhibition of concanavalin A-stimulated lymphocyte proliferation. Biochemical Society Transactions 17: 1042-1043.
Calder, P. C. 2001. N-3 polyunsaturated fatty acids, inflammation and immunity: pouring oil on troubled waters or another fishy tale? Nutrition Research 21:309-341.
Calder, P. C. 2006. Polyunsaturated fatty acids and inflammation. Prostaglandins, Leukotrienes and Essential Fatty Acids 75:197-202.
Caspi, R. R. and R. R. Avtalion. 1984. Evidence for the existence of an IL-2-like lymphocyte growth promoting factor in a bony fish, Cyprinus carpio. Developmental Comparative Immunology 8: 51-60.
Castell, J. D., J. G. Bell, D. R. Tocher and J. R. Sargent. 1994. Effects of purified diets containing different combinations of arachidonic and docosahexaenoic acid on survival, growth and fatty acid composition of juvenile turbot (Scophthalmus maximus). Aquaculture 128:315-333.
Castell, J. D., D. J. Lee and R. O. Sinnhuber. 1972a. Essential fatty acids in the diet of rainbow trout (Salmo gairdneri): lipid metabolism and fatty acid composition. Journal of Nutrition 102:93-100.
Castell, J. D., R .O. Sinnhuber, J. H. Wales and D. J. Lee. 1972b. Essential fatty acids in the diet of rainbow trout (Salmo gairdneri): growth, feed conversion and some gross deficiency symptoms. Journal of Nutrition 102:77-86.
Cinader, B., M. T. Clandinin, T. Hosokawa and N. M. Robblee. 1983. Dietary fat alters the fatty acid composition of lymphocyte membranes and the rate at which suppressor capacity is lost. Immunology Letters 6:331-337.
Copeman, L. A., C. C. Parrish, J. A. Brown and M. Harel. 2002. Effects of docosahexaenoic, eicosapentaenoic, and arachidonic acids on the early growth, survival, lipid composition and pigmentation of yellowtail flounder ( Limanda ferruginea ): a live food enrichment experiment. Aquaculture 210:285-304.
Daly, J. G., A. R. Moore and G. Olivier. 1995. A colorimetric assay for the quantification of brook trout (Salvelinus fontinalis) lymphocyte proliferation. Fish and Shellfish Immunology 5: 265-273.
Davidson, J., A. Kerr, K. Guy and D. Rotondo.1998. Prostaglandin and fatty acid modulation of Escherichia coli O157 phagocytosis by human monocytic cells. Immunology 94:228-234.
De Deckere, E. A., C. J. Verplanke, C. G. Blonk and W. G. van Nielen. 1988. Effects of type and amount of dietary fat on rabbit and rat lymphocyte proliferation in vitro. Journal of Nutrition 118:11-18.
De Kinkelin, P., and M. Dorson. 1973. Interferon production in rainbow trout (Salmo gairdneri, Richardson) experimentally infected with Egtved virus. Journal of General Virology 19: 125-127.
Delaporte M., P. Soudant, J. Moal, E. Giudicelli, C. Lambert, C. S&#233;guineau, and J.-F. Samain. 2006. Impact of 20:4n-6 Supplementation on the fatty acid composition and hemocyte parameters of the pacific oyster Crassostrea gigas. Lipids 41:567-576.
DeLuca, D., M. Wilson and G. Warr. 1983. Lymphocyte heterogeneity in the trout, Salmo gairdneri, defined with monoclonal antibodies to IgM. European Journal of Immunology 13: 546-551.
Erickson, K. L., C. J. McNeill, , M. E. Gershwin and J. B. Ossman. 1980. The influence of dietary fat concentration and saturation on immune ontogeny in mice. Journal of Nutrition 110:1555-1572.
Est&#233;vez, A., M. Ishikawa and A. Kanazawa. 1997. Effects of arachidonic acid on pigmentation and fatty acid composition of Japanese flounder, Paralichthys olivaceus (Temminck and Schelegel). Aquacultur Reserch 28:279-289.
Est&#233;vez, A., L. A. McEvoy, J. G. Bell and J. R. Sargent. 1999. Growth, survival, lipid composition and pigmentation of turbot (Scophthalmus maximus) larvae fed live-prey enriched in arachidonic and eicosapentaenoic acids. Aquaculture 180, 321–343.
Fujii, M., H. Nakayama and Y. Yone. 1976. Effect of n-3 fatty acids on growth, feed efficiency and fatty acid composition of red sea bream (Chyrysophrys major). Report of Fisherise Research Laboratory, Kyushu University 3:65-86.
Fogh K, T. Herlin and K. Kragballe. 1989. Eicosanoids in acute and chronic psoriatic lesions: leukotriene B4, but not 12-hydroxy-eicosatetraenoic acid, is present in biologically active amounts in acute guttate lesions. Journal of Investigative Dermatology 92:837-41.
Folch, J., M. Lees and G. M. Solane. 1957. A simple method for the isolation and purification of total lipids from animal tissues. Journal of Biological Chemistry 191: 497-509.
Fountoulaki, E., M. N. Alexis, I. Nengas and B. Venou. 2003. Effects of dietary arachidonic acid (20:4n-6), on growth, body composition, and tissue fatty acid profile of gilthead seabream fingerlings (Sparus aurata L.). Aquaculture 225: 309-323
Furuita, H., T. Takeuchi, M. Toyota, T. Watanabe. 1996. EPA and DHA requirements in early juvenile red seabream using HUFA enriched Artemia nauplii. Fish Science 62: 246-251.
Furuita, H., T. Yamamoto, T. Shima, N. Suzuki and T. Takeuchi. 2003. Effect of arachidonic acid levels in broodstock diet on larval and egg quality of Japanese flounder paralichthys olivaceus. Aquaculture 220: 725-735.
Ganga, R., J. G. Bell., D. Montero., L. Rpbaina., M. J. Caballero. and M. S. Izquierdo. 2005. Effect of dietary lipids on plasma fatty acid profiles and prostaglandin and leptin production in gilthead seabream (Sparus aurata). Comparative Biochemistry and Physiology 142 :410-418.
Gewirtz, A. T., L. S. Collier-Hyams and A.N. Young. 2002. Lipoxin a4 analogs attenuate induction of intestinal epithelial proinflammatory gene expression and reduce the severity of dextran sodium sulfate-induced colitis. Journal of Immunology 168:5260-5267.
Graham, S., and C. J. Secombes. 1988. The production of a macrophage-activating factor from rainbow trout Salmo gairdneri leucocytes. Immunology 65: 293-297.
Gregory, W. W. 1994. The immunoglobulin genes of fish. Develomenpmental and Comparative Immunology. 19:1-12.
Good, J. E., J. G. Bell, K. D. Thompson and P. W. Williams. 2001. Assessment of immune response in Atlantic salmon (Salmo salar) receiving alternative oil diets. Pages 49-62 in An abstract and poster presentation at the 5th Nordic Fish Immunology Symposium June 2001. Institute of Pharmacy, University of Oslo, Oslo, Norway.
Harbige, L.S. 2003. Fatty acids, the immune response, and autoimmunity: A question of n-6 essentiality and the balance between n-6 and n-3. Lipids 38:323-341.
Hardie, W. D., T. D. Le Cras , K. Jiang, J. W. Tichelaar, M. Azhar, and T. R. Korfhagen. 2004. Conditional expression of transforming growth factor- in adult mouse lung causes pulmonary fibrosis. American Journal of Physiology-Lung Cellular and Molecular Physiology 286:741-749.
Henderson, R. J., M. V. Bell and J. R. Sargent. 1985. The conversion of polyunsaturated fatty acids to prostaglandins by tissue homogenates of the turbot, Scophthalmus maximus, L. Journal of Experimental Marine Biology and Ecology 85:93-99.
Howard, R. W. and D. W. Stanley. 1999. The tie that binds: eicosanoids in invertebrate biology. Annals of the Entomological Society of America 92:880-890.
Hurtado M. A., M. Reza, A. M. Ibarra, M. Wille, P. Sorgeloos, P. Soudant and E. Palacios. 2009. Arachidonic acid (20:4n&#8722;6) effect on reproduction, immunology, and prostaglandin E2 levels in Crassostrea corteziensis (Hertlein, 1951). Aquaculture 294:300-305.
Izquierdo, M. S. 1996. Essential fatty acid requirements of cultured marine fish larvae. Aquaculture Nutrition 2: 183-191.
Iwama, G., and T. Nakanishi. 1996. The fish immune system. Organ, Pathogen, and Environment. Academic Press, San Diego CA, 1-380.
Izquierdo, M. S., T. Watanabe, T. Takeuchi, T. Arakawa and C. Kitajima. 1989. Requirement of larval red seabream Pagrus major for essential fatty acids. Nippon Suisan Gakkaishi 55:859-867.
Jokinen, E. I., H. M. Salo, S. E. Markkula, A. K. Immonen and T. M. Aaltonen. 2001. Ultraviolet B irradiation modulates the immune system of fish (Rutilus rutilus, Cyprinidae). Photochemistry and Photobiology 73:505-512.
Kanazawa, A. 1985. Nutritional factors in fish reproduction. Pages 115-125 in: Reproduction and Culture of Milkfish. (Lee, C.S. & Liao, I.C., editors). The Oceanic Institute, Waimanalo, Hawaii.
Kelley, D. S., G. J. Nelson, C. M. Serrato, P. C. Schmidt and L. B. Branch. 1988. Effects of type of dietary fat on indices of immune status of rabbits. Journal of Nutrition 118:1376-1384.
Kelley, D. S., P. C. Taylor, G. J. Nelson and B. E. Mackey. 1998. Arachidonic acid supplementation enhances synthesis of eicosanoids without suppressing immune functions in young healthy men. Lipids 33:125-130
Kelley, D. S., P. C. Taylor, G. J. Nelson, P. C. Schmidt, B. E. Mackey and D. Kyle. 1997. Effects of dietary arachidonic acid on human immune response. Lipids 32:449-456.
Kelly, J. P. and C. W. Parker. 1979. Effects of arachidonic acid and other unsaturated fatty acids on mitogenesis in human lymphocytes. Journal of Immunology 122:1556-1562.
Kinsella, J. E. and B. Lokesh. 1990. Dietary lipids, eicosanoids and the immune system. Critical Care Medicine 18:94-113.
Koven, W. M., G. W. Kissil and A. Tandler. 1989. Lipid and n-3 requirements of Sparus aurata larvae during starvation and feeding. Aquaculture 79:185-191.
Koven, W. M., A. Tandler, G. W. Kissil and D. Sklam. 1992. The importance of n-3 highly unsaturated fatty acids for growth in larval Sparus aurata and their effect on survival, lipid composition and size distribution. Aquaculture 104:91-104.
Koven W., Y. Barr, S. Lutzky, I. Ben-Atia., R. Weiss, M. Harel, P. Behrens and A. Tandler. 2001a. The effect of dietary arachidonic acid (20:4n-6) on growth, survival and resistance to handling stress in gilthead seabream (Sparus aurata) larvae. Aquaculture 193:107-122.
Koven, W., R. Van Anholt, S. Lutzky, I. Ben-Atia, K. Gamsiz, R. Weiss and A. Tandler. 2001b. The importance of arachidonic acid, as a modulator of stress resistance through the hypothalamus –pituitary–interrenal axis, in different aged gilthead seabream larvae. Fish and Shellfish Larviculture Symposium European Aquaculture Society 30:292-293.
Koven, W. M., R. Van Anholt, S. Lutsky, I. Ben Atia, O. Nixon, B. Ron, A. Tandler. 2003. The effect of dietary arachidonic acid on growth, survival, and cortisol levels in different-age gilthead seabream larvae (Sparus auratus) exposed to handling or daily salinity change. Aquaculture 228:307-320.
Lands, W. E. M., A. Morris and B. Libelt. 1990. Quantitative effects of dietary polyunsaturated fats on the composition of fatty acids in rat tissues. Lipids 25:505-516.
Lee, S.-M. 2001. Review of the lipid and essential fatty acid requirements of rockfish (Sebastes schlegeli). Aquaculture 32:8-17.
Lemaire-Gony, S., P. Lemaire and A. L. Pulsford. 1995. Effects of cadmium and benzo (a) pyrene on the immune system, gill ATPase and EROD activity of European sea bass Dicentrarchus labrax. Aquatic Toxicology 31: 297-313.
Lennartz, M. R. 1999. Phospholipases and Phagocytosis: the role of phospholipid-derived second messengers in phagocytosis. International Journal of Biochemistry and Cell Biology 31:415-430.
Levy, B. D., C. B. Clish, B. Schmidt, K. Gronert and C.N. Serhan. 2001. Lipid mediator class switching during acute inflammation: signals in resolution. Nature Immunology 2:612-619.
Li, Y., J. X. Kang and A. Leaf. 1997. Differential effects of various eicosanoids on the production or prevention of arrhythmias in cultured neonatal rat cardiac myocytes. Prostaglandins 54:511-530.
Lie, O., E. Lied and G. Lambertsen. 1986. Liver retention of fat and of fatty acids in cod (Gadus morhua) fed different oils. Aquaculture 59:187-196
Lin, Y. H. and S.Y. Shiau 2003. Dietary lipid requirement of grouper, Epinephelus malabaricus, and effects on immune responses. Aquaculture 225:243-250.
Lloret, S., and J. J. Moreno.1996. Role of kinases and g-Proteins on arachidonate release induced by zymosan in mouse peritoneal macrophages. International Journal of Biochemistry and Cell Biology 28:465-472.
Locniskar, M., K. M. Nauss and P. M. Newberne. 1983. The effect of quality and quantity of dietary fat on the immune system. Journal of Nutrition 113:951-961.
Lovell, R.T. (editor) 1998. Dietary requirements. Pages 13-70 in: Nutrition and Feeding of Fish. 2nd edition, Kluwer Academic, London.
Lund, I., S. J. Steenfeldt and B. W. Hansen. 2007. Effect of dietary arachidonic acid, eicosapentaenoic acid and docosahexaenoic acid on survival, growth and pigmentation in larvae of common sole (Solea solea L.). Aquaculture 272:532-544.
Lund, I., S. J. Steenfeldt, G. Banta and B.W.Hansen. 2008. The influence of dietary concentrations of arachidonic acid and eicosapentaenoic acid at various stages of larval ontogeny on eye migration, pigmentation and prostaglandin content of common sole larvae (Solea solea L.). Aquaculture 276:143-153.
Mackie, A. M. and A. I. Mitchell. 1985. Identification of gustatory feeding stimulants for fish--applications in aquaculture. Pages 177-189 in: Nutrition and Feeding in Fish. (Cowey, C.B., Mackie, A.M., & Bell, J.B., editors). Academic Press, London.
Madhavi, N., U. N. Das, P. S. Prabha, G. S. Kumar, R. Koratkar and P. S. Sagar. 1994. Suppression of human T-cell growth in vitro by cis-unsaturated fatty acids: relationship to free radicals and lipid peroxidation. Prostaglandins Leukotrienes and Essential Fatty Acids 51: 33-40.
Mancuso, P., T. J. Standiford, T. Marshall, and M. Peters-Golden. 1998. 5-Lipoxygenase reaction products modulate alveolae macrophage phagocytosis of Klebsiella pneumonia. Infection and Immunity 66:5140-5146.
Marszalek, J. R. and H. F. Lodish. 2005. Docosahexaenoic acid, fatty acid-interacting proteins, and neuronal function: breastmilk and fish are good for you. Annual Review of Cell and Developmental Biology 21:633-657.
Matsuyami, H., R. E. P. Mangindaan and T. Yano. 1992. Protective effect of schizophyllan and scleoglucan against Streptococcus spp. Infection in yellowtail (Seriola quinqueradiata). Aquaculture 101:197-203.
Mertin, J. and R. Hunt. 1976. Influence of polyunsaturated fatty acids on survival of skin allografts and tumor incidence in mice. Proceeding National Academic Science, USA 73:928-931.
Mourente, G., A. Rodr′&#305;guez, D. R. Tocher and J. R. Sargent. 1993. Effects of dietary docosahexaenoic acid DHA; 22:6 n-3. on lipid and fatty acid compositions and growth in gilthead sea bream (Sparus aurata L.) larvae during first feeding. Aquaculture 112:79-98.
Mustafa, T. and K. C. Srivastava. 1989. Prostaglandins (eicosanoids) and their role in ectothermic organisms. Advances in Comparative and Environmental 5:157-207.
Nakanishi, Y., H. Kodama, T. Murai and H. Izawa. 1991. Activation of rainbow trout complement by C-reactive protein. American Journal of Veterinary Research 52:397-401.
Nematipour, G. R. and D .M. Gatlin. 1993. Requirement of hybrid striped bass for dietary (n-3) highly unsaturated fatty acids. Journal of Nutrition 123:744-753.
Newberry, R. D., W. Stenson and R. G. Lorenz. 1999. Cyclooxygenase-2-dependent arachidonic acid metabolites are essential modulators of the intestinal immune response to dietary antigen. Nature Medicine 5:900-906
Nghia, T. T., M. Wille and P. Sorgeloos. 2001. Influence of the content and ratio of essential HUFA’s in the live food on larviculture success of the mud crab (Scylla paramamosain). Fish and Shellfish Larviculture Symposium European Aquaculture Society 30:430-433.
Novoa, B., A. Figueras, I. Ashton and C. J. Secombes. 1996. In vitro studies on the regulation of rainbow trout (Oncorhynchus mykiss) macrophage respiratory burst activity. Developmental and Comparative Immunology 20:207-216.
Ways, P. and D. J. Hanahan. 1964. Characterization and quantification of red cell lipids in normal man. Journal of Lipid Research 5:318-328.
Weber, P. C., S. Fischer, S. C. von, R. Lorenz and T. Strasser. 1986. The conversion of dietary eicosapentaenoic acid to prostanoids and leukotrienes in man. Progress in lipid research 25:273-6.
Webster, C. D. and R. T. Lovell. 1990. Response of striped bass larvae fed braine shrimp from different sources containing different fatty acid compositions. Aquaculture 90:49-61.
Webster, C. D., R.T. Lovell and J. A. Clawson. 1994. Ratio of 20:3(n-9) to 20:5(n-3) in phospholipids as an indicator of dietary essential fatty acid sufficiency in striped bass, Morone saxatilis, and palmetto bass, M. saxatilis × M. chrysops. Journal of Applied Aquaculture 4:75-90.
Wu, F. C., Y. Y. Ting and H. Y. Chen. 2002. Docosahexaneoic acid is superior to eicosapentaenoic acid as the essential fatty acid for growth of grouper, Epinephelus malabaricus. Journal of Nutrition 132:72-79.
Wu, F. C., Y. Y. Ting and H. Y Chen. 2003. Dietary docosahexaenoic acid is more optimal than eicosapentaenoic acid affecting the level of cellular defence responses of the juvenile grouper Epinephelus malabaricus. Fish and shellfish Immunology 14:223-238.
Obach, A., C. Quentel and F. Bandin Laurencin. 1993. Effects of alpha-tocopherol and dietary oxidized fish oil on the immune response of sea bass Dicentrarchus labra. Diseases of Aquatic Organisms 15:175-185.
Olson, L. M., S. K. Clinton, J. I. Everitt, P. V. Johnston and W. J. Visek. 1987. Lymphocyte activation, cell-mediated cytotoxicity and their relationship to dietary fat-enhanced mammary tumorigenesis in C3H/OUJ mice. Journal of Nutrition 117:955-963.
Owen, J. M., J. W. Adron, C. Middleton and C. B. Cowey. 1975. Elongation and desaturation of dietary fatty acids in turbot, Scophthalmus maximus L., and rainbow trout, Salmo gairdneri Richard. Lipids 10:528-531.
Plouffe, D. A., P. C. Hanington., J. G. Walsh., E. C. Wilson. and M. Belosevic. 2005. Comparison of select innate immune mechanisms of fish and mammals. Xenotransplantation 12: 266-277.
Plumb, J. A., R. Milroy and S. B. Kaye. 1989. Effects of the pH dependence of 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-tetrazolium bromide-formazon absorption on chemosensitivity determined by a novel tetrazolium-based assay. Cancer Research 49: 4435-4440.
Rezek, T. C., W. O. Watanabe, M. Harel and P. J. Seaton. 2009. Effects of dietary docosahexaenoic acid (22:6n&#8722;3) and arachidonic acid (20:4n&#8722;6) on the growth, survival, stress resistance and fatty acid composition in black sea bass Centropristis striata (Linnaeus 1758) larvae. Aquacuture Research 10: 1365-2109.
Rodriguez, C., J. A. Perez, M. S. Izquierdo, J. Mora, A. Lorenzo and H. Ferna′ndez-Palacios. 1993. Essential fatty acid requirements for larval gilthead sea bream (S. aurata.) Aguaculture and Fisheries Management 24: 295-304.
Rodriguez, C. 1994. Estudio de los requerimientos de a′cidos grasos esenciales de la dorada europea (Sparus aurata, L.) durante las dos primeras semanas de alimentacio′n. PhD Thesis, University of La Laguna, Spain.
Rodriguez, C., J. A.Perez, M. S. Izquierdo, J. Mora, A. Lorenzo and H. Fernandez-Palacious. 1994. Essential fatty acid requirements of larval gilthead sea bream, Sparus aurata (L.). Aquaculture and Fisheries Management 25:295-304.
Salhi, M., M. S. Izquierdo, C. M. Hern′andez-Cruz, M. Gonz′alez and H. Fern′andez-Palacios. 1994. Effect of lipid and n-3 HUFA levels in microdiets on growth survival and fatty acid composition of larval gilthead seabream (Sparus aurata) Aquaculture 124:275-282.
Sargent, J. R., R. J. Henderson and D. R. Tocher. 1989. The lipids. Academic Press, San Diego, CA.
Sargent, J. R., J. G. Bell, M. V. Bell, R. J. Henderson and D. R. Tocher. 1995. Requirement criteria for essential fatty acids. Jounal of Applied Ichthyology 11:183-198.
Sargent, J. R., J. G. Bell, L. A. McEvoy, D. R. Tocher and A. Estevez. 1998. Recent developments in the essential fatty acid nutrition of fish. Aquaculture 177:191-199.
Sargent, J. R., L. A. McEvoy, A. Estevez, J. G. Bell, M. V. Bell, R. J. Henderson and D. R. Tocher. 1999. Lipid nutrition of marine fish during early development: current status and future directions. Aquaculture 179:217-229.
Schmitz, G. and J. Ecker. 2008. The opposing effects of n-3 and n-6 fatty acids. Progress in lipid research 47:147-155.
Secombes, C. J. 1990. Isolation of salmonid macrophages and analysis of their killing activity. Pages 137-154 in: Techniques in Fish Immunology 1. (Stolen, J.S., Fletcher, T.C., Anderson, D.P., Robertson, B.S., and van Muiswinkel, W.B., editors). SOS Publications, Fair Haven, NJ.
Secombes, C. J. and T. C. Fletcher. 1992. The role of phagocytes in the protective mechanisms of fish. Annual Review of Fish Diseases 2:53-71.
Secombes, C. J. 1994. Enhancement of fish phagocyte activity. Fish and Shellfish Immunology 4:421-436.
Secombes, C. J., K. Clements, I. Ashton and A. F. Rowley. 1994. The effects of eicosanoids on rainbow trout, Oncorhynchus mykiss, leucocyte proliferation. Veterinary Immunology and Immunopathology 42:367-378.
Secombes, C. J. 1996. The nonspecific immunity system: Cellular defenses. Pages 63-105 in: The Fish Immune System. (Iwama, G.K. and Nakanishi, T., editors) Academic Press, San Diego, CA.
Secombes, C. J., J. Zou, K. Laing, and G. D. Daniel. 1999. Cytokine genes in fish. Aquaculture 172: 93-102.
Seierstad, S. L., &#216;. Haugland, S. Larsen, R. Waagb&#248; and &#216;. Evensen. 2009. Pro-inflammatory cytokine expression and respiratory burst activity following replacement of fish oil with rapeseed oil in the feed for Atlantic salmon (Salmo salar L.). Aquaculture 289:212-218
Serhan, C. N., A . Jain and S. Marleau. 2003. Reduced inflammation and tissue damage in transgenic rabbits overexpressing 15-lipoxygenase and endogenous anti-inflammatory lipid mediators. Journal of Immunology 171:6856-65.
Sharp, G. J. E., T. R. Pettitt, A. F. Rowley and C. J. Secombes. 1992. Lipoxin-induced migration of fish leukocytes. Journal of Leukocyte Biology 51:140-145.
Sizemore, R. C., N. W. Miller, M. A. Cuchens, C. J. Lobb and L. W. Chem. 1984. Phylogeny of lymphocyte heterogeneity: the cellular requirements for in vitro mitogenic responses of channel catfish leucocytes. Journal of Immunology 133: 2920-2924.
Solem, S. T., J. B. Jorgensen and B. Robertsen. 1995. Stimulation of respiration of respiratory burst and phagocytic activity in Atlantic salmon (Salmo salar L.) macrophages by lipopolysaccharide. Fish and Shellfish Immunology 5:475-491.
Song, Y. L., and Y. T. Hsieh. 1994. Immunostimulation of tiger shrimp (Penaeus monodon) hemocytes for generation of microbicidal substances: analysis of reactive oxygen species. Developmental and Comparative Immunology 18:201–209.
Stowell, S. L. and D. M. Gatlin. 1992. Effects of dietary pantethine and lipid levels on growth and body composition of channel catfish, Ictalurus punctatus. Aquaculture 108:177-188.
Subhadra, B., R. Lochmann., S. Rawles. and R. Chen. 2006. Effect od dietary lipid source on the growth, tissue composition and hematological parameters of largrmouth bass (Micropterus salmoides). Aquaculture 255:210-222.
Tafalla, C., A. Figueras and B. Novoa. 2002. Possible role of LTB4 in the antiviral activity of turbot (Scophthalmus maximus) leukocyte-derived supernatants against viral hemorrhagic septicemia virus (VHSV). Fish and shellfish Immunology 26:283-293.
Tocher, D. R. and J. R. Sargent. 1987. The effect of calcium ionophore A23187 on the metabolism of arachidonic and eicosapentaenoic acids in neutrophils from a marine teleost fish rich in n-3 polyunsaturated fatty acids. Comparative Biochemistry and Physiology 67: 733-739.
Tocher, D. R. 1993. Elongation predominates over desaturation in the metabolism of 18:3n-3 and 20:5n-3 in turbot (Scophthalmus maximus) brain astroglial cells in primary culture. Lipids 28:267-272.
Tocher, D. R., J. G. Bell, J. R. Dick, R. J. Henderson, F. McGhee, D. Mitchell and P. C. Morris. 2000. Polyunsaturated fatty acid metabolism in Atlantic salmon (Salmo salar) undergoing parr– smolt transformation and the effects of dietary linseed and rapeseed oils. Fish Physiology and Biochemistry 23:59-73.
Tocher, D. R., J. G. Bell, P. MacGlaughlin, F. McGhee and J. R. Dick. 2001. Hepatocyte fatty acid desaturation and polyunsaturated fatty acid composition of liver in salmonids: effects of dietary vegetable oil. Comparitive Biochemistry and Physiology 130:257-270.
Tocher, D. R. 2003. Metabolism and functions of lipids and fatty acids in teleost fish. Reviews in Fisferies Science. 11:107-184.
Vachier, I., P. Chanez and C. Bonnans. 2002. Endogenous anti-inflammatory mediators from arachidonate in human neutrophils. Biochemical and Biophysical Research Communications 290:219-24.
Van Anholt, R. D., W. M. Koven, S. Lutzky and S.E.W. Bonga. 2004. Dietary supplementation with arachidonic acid alters the stress response of gilthead seabream (Sparus aurata) larvae. Aquaculture 238: 369-383.
Verlengia, R., R. Gorja&#732;o, C. C. Kanunfre, S. Bordin, T. M. Lima and R. Curi. 2003. Effect of arachidonic acid on proliferation, cytokines production and pleiotropic genes expression in Jurkat cells—A comparison with oleic acid. Life Sciences 73:2939-2951
Villalta, M., A. Est&#233;vez and M. P. Bransden, , 2005. Arachidonic acid enriched live prey induces albinismin Senegal sole (Solea senegalensis) larvae. Aquaculture 245: 193-209.
Watanabe, T., 1993. Importance of docosahexanoic acid in marine larval fish. Journal of the World Aquaculture Society 24:152-161.
Xu, H., Q. Ai, K. Mai, W. Xu, J. Wang, H. Ma, W. Zhang, X. Wang and Z. Liufu. 2010. Effects of dietary arachidonic acid on growth performance, survival, immune response and tissue fatty acid composition of juvenile Japanese seabass, Lateolabrax japonicas. Aquaculture 307:75-82.
Yano, T. 1995. Fish complement. Fish Pathology 30:151-158.
Yano, T. 1996. The non-specific immune system: humoral defenses. Pages 105-157 in: Iwama, G., and Nakanishi, T. (Eds), The fish immune system. Organism, Pathogen, and Emvironment. Academic Press, San Diego, California, USA.
Yano, T. 2000. The immune system. Pages 41-61 in Chen, J. C., and Chen, S. Y. (Eds.), Progess in fish Immunlogy. Peoceedings of the Fish Immunology Symposium: Progress in Fish Immunology. Nation Taiwan Ocean university, Keelung, Taiwan.
Yone, Y. 1978. Essential fatty acid requirements of marine fish. Pages 43-59 in Japane Society Science of Fish. Dietary Lipids in Aquaculture. Koseisha-Koseik-Abu, Tokyo.